{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T19:21:36Z","timestamp":1790968896860,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,9,17]],"date-time":"2024-09-17T00:00:00Z","timestamp":1726531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004530","name":"Universiti Putra Malaysia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Extended\u2010spectrum \u03b2\u2010lactamase\u2010producing <jats:italic>Escherichia coli<\/jats:italic> (ESBL\u2010EC) are widespread multidrug\u2010resistant zoonotic bacteria that threaten animal production, food safety and antimicrobial therapy worldwide including Malaysia. Poultry has been reported as one of the pathways for human exposure to ESBL\u2010EC. There has been little research on the occurrence of ESBL\u2010EC within the Malaysian poultry food chain. Hence, the objectives of the study were to determine the occurrence of ESBL\u2010EC in chickens and to identify the potential risk factors associated with their occurrence in poultry farms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A cross\u2010sectional study was conducted on 400 samples, consisting of 240 cloacal swabs from chickens and 160 from poultry farms environments in eight districts in Selangor, Malaysia using culture and disk combination methods and multiple polymerase chain reaction assays. In the determination of possible factors associated with the presence of ESBL\u2010EC at poultry farms, a questionnaire was used to obtain the information and data.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The findings demonstrated the wide distribution of ESBL\u2010EC in all the farms with an overall occurrence of 37.2%. Farms in Gombak, Klang and Hulu Selangor had the highest occurrence rates at 62%, 50% and 50%, respectively, followed by farms in Petaling 38%, Sepang at 34%, Kuala Langat at 26% and Kuala Selangor at 24%, and the lowest was in Hulu Langat 14%. Among the study samples, chickens had the highest occurrence rate at 45.4%, followed by chicken house floors at 40% and flies at 30%, while feed and water samples at 17.5% and 12.5%, respectively. The present study indicated the high occurrence and wide dissemination of ESBL\u2010EC in chickens and poultry farms environment. The ESBL\u2010EC occurrence was associated with several factors including imprudent use of antibiotics, poor husbandry, management and biosecurity practices at the farms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our study showed the presence and spread of ESBL EC among chickens in the farms and their environment; this may lead to being spread to outside of farm environment by flies, vermins, flying birds, litter and farm wastes and possibly to humans upon contact with the contaminated environment and by poultry meat. Thus, the findings of the study can assist to serve as a piece of useful information to veterinary authority in designing evidence\u2010based mitigation strategies for the control of ESBL\u2010EC in poultry farms.<\/jats:p><\/jats:sec>","DOI":"10.1111\/zph.13179","type":"journal-article","created":{"date-parts":[[2024,9,18]],"date-time":"2024-09-18T05:19:37Z","timestamp":1726636777000},"page":"962-971","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["<scp>ESBL<\/scp> Producing <i>E. coli<\/i> in Chickens and Poultry Farms Environment in Selangor, Malaysia: A Cross\u2010Sectional Study on Their Occurrence and Associated Risk Factors With Environment and Public Health Importance"],"prefix":"10.1111","volume":"71","author":[{"given":"Abdulrasheed\u00a0Bello","family":"Aliyu","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathology and Microbiology Universiti Putra Malaysia  Serdang Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abu","family":"Jalila","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine Universiti Putra Malaysia  Serdang Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul\u00a0Aziz","family":"Saleha","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology and Microbiology Universiti Putra Malaysia  Serdang Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6574-0841","authenticated-orcid":false,"given":"Zakaria","family":"Zunita","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology and Microbiology Universiti Putra Malaysia  Serdang Selangor Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,9,17]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s43094-022-00406-8"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12889-016-3377-2"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3109\/03009734.2014.901445"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0135402"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.336"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq222"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.5398\/tasj.2022.45.1.16"},{"key":"e_1_2_10_9_1","volume-title":"Performance Standards for Antimicrobial Disk Susceptibility Tests","author":"Clinical and Laboratory Standards Institute","year":"2010"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.03.029"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01545-10"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks349"},{"key":"e_1_2_10_13_1","unstructured":"FAO.2023. \u201cFAO's Role in Addressing Antimicrobial Resistance.\u201dhttp:\/\/www.fao.org\/antimicrobial\u2010resistance\/en\/. 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