{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T17:30:10Z","timestamp":1790098210343,"version":"4.0.1"},"reference-count":49,"publisher":"Wiley","license":[{"start":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T00:00:00Z","timestamp":1728864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Antimicrobial resistance (AMR) is one of the leading public health threats globally. AMR genes can be transferred between bacteria through lateral gene transfer, and AMR organisms can spread through environments by contaminated water, agriculture and animals. Thus, widespread environmental dissemination of bacteria and lateral gene transfer facilitate AMR transmission pathways. Farm environments in dairy and calf production are known to harbour AMR bacteria that pose a risk for food contamination and to workers in direct or indirect contact with animals. <jats:italic>Escherichia coli<\/jats:italic> is present in farm environments and is known to participate in lateral gene transfer, providing a good marker of resistance genes in each environment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, <jats:italic>E. coli<\/jats:italic> from nine cohorts of calves was isolated at different time points from nine barns, nine trailers and one slaughterhouse environment in a single special\u2010fed veal calf production facility. The antimicrobial susceptibility to 15 antimicrobials, classified as highly or critically important by the World Health Organization, was characterised for <jats:italic>E. coli<\/jats:italic> isolates using Kirby\u2013Bauer disk diffusion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The highest proportion of isolates showing multidrug resistance was present in barn environments (51.7%), where calves were housed from their arrival at &lt;\u20092\u2009weeks of age until they were transported to slaughter. Additionally, 15 <jats:italic>E. coli<\/jats:italic> isolates were resistant to 11 of the 15 antimicrobials tested. Trailer and slaughterhouse environments had greater prevalence of resistance after accommodating calves, including resistance to third\u2010generation cephalosporins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These data highlight the importance of calf environments in the dissemination of resistant bacteria and gives insight into where interventions could be most effective in combatting antimicrobial\u2010resistant bacteria that could infect humans and livestock.<\/jats:p><\/jats:sec>","DOI":"10.1111\/zph.13186","type":"journal-article","created":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T04:59:18Z","timestamp":1728968358000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Characterisation of Antimicrobial Resistance in Special\u2010Fed Veal Production Environments"],"prefix":"10.1111","author":[{"given":"Alexander","family":"Dunmyre","sequence":"first","affiliation":[{"name":"Department of Veterinary Preventative Medicine The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Poonam","family":"Vinayamohan","sequence":"additional","affiliation":[{"name":"Department of Veterinary Preventative Medicine The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha\u00a0R.","family":"Locke","sequence":"additional","affiliation":[{"name":"Department of Veterinary Preventative Medicine The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3732-9147","authenticated-orcid":false,"given":"Ting\u2010Yu","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Veterinary Preventative Medicine The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Schaffner","sequence":"additional","affiliation":[{"name":"Department of Veterinary Preventative Medicine The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0855-7650","authenticated-orcid":false,"given":"Greg","family":"Habing","sequence":"additional","affiliation":[{"name":"Department of Veterinary Preventative Medicine The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,14]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866\u2010019\u20101576\u20106"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.4315\/jfp\u201022\u2010178"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v104.i03"},{"key":"e_1_2_10_5_1","unstructured":"BioRender. n.d.\u201cScientific Image and Illustration Software.\u201dhttps:\/\/www.biorender.com\/."},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.310.6973.170"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa345"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/aac.02412\u201014"},{"key":"e_1_2_10_9_1","unstructured":"Center for Genomic Epidemiology. n.d.\u201cCenter for Genomic Epidemiology.\u201dhttps:\/\/www.genomicepidemiology.org\/."},{"key":"e_1_2_10_10_1","volume-title":"Antibiotic Resistance Threats in the United States","author":"CDC","year":"2019"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.01416"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/zph.12928"},{"key":"e_1_2_10_13_1","volume-title":"CLSI M100","author":"Clinical and Laboratory Standards Institute","year":"2023"},{"key":"e_1_2_10_14_1","volume-title":"CLSI VET01S","author":"Clinical and Laboratory Standards Institute","year":"2023"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed4010022"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/1541\u20104337.12438"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2003.10.017"},{"key":"e_1_2_10_18_1","volume-title":"Extralabel use and antimicrobials","author":"FDA","year":"2022"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.622495"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1207\/S15328031US0104_02"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2015.09.001"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2017.2310"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.2307\/2533558"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2021.04.024"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/aac.47.10.3222\u20103232.2003"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2017.0226"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010food\u2010041715\u2010033251"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.4315\/jfp\u201021\u2010410"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469\u20100691.2011.03570.x"},{"key":"e_1_2_10_30_1","volume-title":"Clinical Veterinary Microbiology","author":"Markey B. K.","year":"2013"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.arba\u20100009\u20102017"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3382\/ps.0702429"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0140\u20106736(21)02724\u20100"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.082188899"},{"key":"e_1_2_10_35_1","volume-title":"National Poultry Improvement Plan Program Standards","author":"USDA","year":"2017"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2015.09.029"},{"key":"e_1_2_10_37_1","unstructured":"Underwood A.2020.\u201cGHRU (Genomic Surveillance of Antimicrobial Resistance) Retrospective 1 Bioinformatics Methods v.4.\u201dCenter for Genomic Pathogen Surveillance.https:\/\/www.protocols.io\/view\/ghru\u2010genomic\u2010surveillance\u2010of\u2010antimicrobial\u2010resista\u2010bp2l6b11kgqe\/v4."},{"key":"e_1_2_10_38_1","volume-title":"Bovine Environmental Sampling Instructions for Salmonella spp","author":"University of Wisconsin","year":"2018"},{"key":"e_1_2_10_39_1","unstructured":"USDA Food Safety and Inspection Service.2013.\u201cSafe Food Handling and Preparation | Food Safety and Inspection Service.\u201dhttps:\/\/www.fsis.usda.gov\/food\u2010safety\/safe\u2010food\u2010handling\u2010and\u2010preparation."},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dky417"},{"key":"e_1_2_10_41_1","volume-title":"Two Heads Are Better Than One: A Starter Guide to Pairing Dairy Calves","author":"Van Os J.","year":"2021"},{"key":"e_1_2_10_42_1","volume-title":"Veal Quality Assurance Certification Resource Manual","author":"Veal Quality Assurance Program","year":"2018"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2017.2406"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10081652"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.07.020"},{"key":"e_1_2_10_46_1","volume-title":"A Laboratory Manual for the Isolation, Identification and Characterization of Avian Pathogens","author":"Williams S. M.","year":"2016"},{"key":"e_1_2_10_47_1","volume-title":"Critically Important Antimicrobials for Human Medicine","author":"WHO","year":"1970"},{"key":"e_1_2_10_48_1","volume-title":"Critically Important Antimicrobials for Human Medicine 24\u201325","author":"WHO","year":"2018"},{"key":"e_1_2_10_49_1","volume-title":"Antimicrobial Resistance","author":"WHO","year":"2021"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks496"}],"container-title":["Zoonoses and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/zph.13186","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T04:59:26Z","timestamp":1728968366000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/zph.13186"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,14]]},"references-count":49,"alternative-id":["10.1111\/zph.13186"],"URL":"https:\/\/doi.org\/10.1111\/zph.13186","archive":["Portico"],"relation":{},"ISSN":["1863-1959","1863-2378"],"issn-type":[{"value":"1863-1959","type":"print"},{"value":"1863-2378","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,14]]},"assertion":[{"value":"2023-11-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"zph.13186"}}