{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T20:45:56Z","timestamp":1772311556357,"version":"3.50.1"},"reference-count":62,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T00:00:00Z","timestamp":1733097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005001","name":"Ahvaz Jundishapur University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p><jats:italic>Toxoplasma gondii<\/jats:italic> is a parasitic protozoan capable of infecting warm\u2010blooded animals, including humans, and is the causative agent of toxoplasmosis. Wild boars (<jats:styled-content style=\"fixed-case\"><jats:italic>Sus scrofa<\/jats:italic><\/jats:styled-content>) serve as reservoirs for zoonotic pathogens like <jats:italic>T. gondii<\/jats:italic>, which can cause disease in humans if transmitted. This study aimed to assess the seroprevalence and molecular prevalence of <jats:italic>T. gondii<\/jats:italic> in wild boars in Khuzestan Province, Iran.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Seroprevalence of <jats:italic>T. gondii<\/jats:italic> antibodies was assessed using enzyme\u2010linked immunosorbent assay (ELISA), employing a commercial kit (Calbiotech), whereas molecular detection was carried out through polymerase chain reaction (PCR) targeting the <jats:italic>B1<\/jats:italic> gene of <jats:italic>T. gondii<\/jats:italic> on tissue samples (tongue, muscle, diaphragm and heart). Thirty wild boars were sampled from the regions of Shush, Shushtar, Dezful and Abadan, and both blood and tissue samples were analysed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In this study, the seroprevalence of <jats:italic>T. gondii<\/jats:italic> antibodies was found to be 83.3% (25\/30) among wild boar serum samples using ELISA. Molecular detection through PCR identified <jats:italic>T. gondii<\/jats:italic> DNA in 46.7% (14\/30) of tissue samples, with the highest detection rates in tongue tissues (64%), followed by muscle (21%) and diaphragm (14%). No positive results were found in heart samples, and no animal had multiple tissues testing positive. All PCR\u2010positive cases corresponded with positive ELISA results, and a statistically significant difference was observed in parasite prevalence across different tissues (<jats:italic>p<\/jats:italic>\u2009=\u20090.002).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Although consumption of wild boar meat is banned in Iran, illegal hunting and consumption remain a concern. The high prevalence of <jats:italic>T. gondii<\/jats:italic> in wild boars poses a potential risk for transmission through the illegal consumption of undercooked or raw meat. This study highlights the need for public health interventions to control the illegal trade of wild boar meat and reduce the risk of toxoplasmosis transmission. Further research is recommended to investigate <jats:italic>T. gondii<\/jats:italic> distribution in other tissues, including the brain, and to better understand the parasite's epidemiology in this region.<\/jats:p><\/jats:sec>","DOI":"10.1111\/zph.13195","type":"journal-article","created":{"date-parts":[[2024,12,2]],"date-time":"2024-12-02T09:55:10Z","timestamp":1733133310000},"page":"166-173","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Seroprevalence and Molecular Detection of <i>Toxoplasma gondii<\/i> in Wild Boars (<i>Sus scrofa<\/i>) in Khuzestan Province, Iran"],"prefix":"10.1111","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2497-6158","authenticated-orcid":false,"given":"Mohammad Javad","family":"Boozhmehrani","sequence":"first","affiliation":[{"name":"Department of Medical Parasitology, Faculty of Medicine Jundishapur University of Medical Sciences  Ahvaz 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