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It favours habitats such as shrubs, deciduous and mixed forests, but can also be found in urban environments. Due to its high vector competence, it is of enormous veterinary as well as medical importance, transmitting tick\u2010borne encephalitis (TBE) virus, <jats:styled-content style=\"fixed-case\"><jats:italic>Borrelia burgdorferi<\/jats:italic><\/jats:styled-content> s. l., the causative agent of lyme borreliosis, <jats:italic>Rickettsia<\/jats:italic> spp. and <jats:styled-content style=\"fixed-case\"><jats:italic>Anaplasma phagocytophilum<\/jats:italic><\/jats:styled-content> amongst many other pathogens. In Kosovo, <jats:styled-content style=\"fixed-case\"><jats:italic>I. ricinus<\/jats:italic><\/jats:styled-content> is the predominant species and a few studies, mostly based on human and animal seroprevalences, indicate the circulation of tick\u2010borne pathogens. However, data on pathogens in <jats:styled-content style=\"fixed-case\"><jats:italic>I. ricinus<\/jats:italic><\/jats:styled-content> are scarce in Kosovo, particularly in urban settings. This study aimed to provide first insights into the circulation of tic\u2010kborne pathogens in <jats:italic>I. ricinus<\/jats:italic> from urban and peri\u2010urban areas in Kosovo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Urban and peri\u2010urban areas were sampled by flagging 150 m transects. In total, 197 ticks were morphologicaly identified as <jats:italic>I. ricinus<\/jats:italic>, and consequently DNA and RNA were isolated. All individuals were screened for the presence of tick\u2010borne pathogens by using reverse line blotting (RLB) hybridisation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>DNA of nine different pathogens from four genera including <jats:italic>Borrelia<\/jats:italic> spp., <jats:italic>Rickettsia<\/jats:italic> spp., <jats:italic>Anaplasma<\/jats:italic> spp. and <jats:italic>Babesia<\/jats:italic> spp. was detected in 60 (33.5%) specimens. The most frequently detected pathogens were <jats:italic>Rickettsia<\/jats:italic> spp. (16.2%), followed by <jats:italic>Borrelia<\/jats:italic> spp. (11.7%). Altogether, 54 single infections, 11 double infections and 1 triple infection were observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We provide first data on genotyping of <jats:styled-content style=\"fixed-case\"><jats:italic>B. burgdorferi<\/jats:italic><\/jats:styled-content> sensu lato as well as the detection of <jats:italic>Anaplasma<\/jats:italic>, <jats:italic>Babesia<\/jats:italic> and <jats:italic>Rickettsia<\/jats:italic> from <jats:styled-content style=\"fixed-case\"><jats:italic>I. ricinus<\/jats:italic><\/jats:styled-content> in this country. The data underline that particularly recreational (peri\u2010)urban areas could facilitate the spillover of zoonotic tick\u2010borne pathogens to humans in Kosovo and provide baseline data for future surveys.<\/jats:p><\/jats:sec>","DOI":"10.1111\/zph.13197","type":"journal-article","created":{"date-parts":[[2024,12,9]],"date-time":"2024-12-09T07:35:39Z","timestamp":1733729739000},"page":"174-183","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Zoonotic Tick\u2010Borne Pathogens in <i>Ixodes ricinus<\/i> Complex (Acari: Ixodidae) From Urban and Peri\u2010Urban Areas of Kosovo"],"prefix":"10.1111","volume":"72","author":[{"given":"Ina","family":"Hoxha","sequence":"first","affiliation":[{"name":"Institute of Specific Prophylaxis and Tropical Medicine, Center for Pathophysiology, Infectiology and Immunology Medical University Vienna  Vienna 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