{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T06:16:10Z","timestamp":1782627370136,"version":"3.54.5"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008968","name":"National Research Council Sri Lanka","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008968","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Extraintestinal pathogenic <jats:styled-content style=\"fixed-case\"><jats:italic>Escherichia coli<\/jats:italic><\/jats:styled-content> (ExPEC) is an important human and animal pathogen. In particular, the ST131 of ExPEC is a widely distributed clone, resistant to extended\u2010spectrum cephalosporins and fluoroquinolones. We investigated the occurrence of ST131 among ExPEC from humans and dogs in Sri Lanka and determined its antimicrobial resistance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 215 ExPEC isolates were collected from humans (<jats:italic>n<\/jats:italic>\u2009=\u2009179) and dogs (<jats:italic>n<\/jats:italic>\u2009=\u200936) from two different cities in Sri Lanka. Antimicrobial resistance was tested by the disk diffusion method, and the presence of resistance\u2010encoding genes (<jats:italic>bla<\/jats:italic><jats:sub>TEM<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>SHV<\/jats:sub> and <jats:italic>bla<\/jats:italic><jats:sub>CTX\u2010M<\/jats:sub>) and ST131 clades\/subclades was tested by PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The majority of the isolates were (61.8%) ST131, with 55.8% in ST131\u2010clade C. Of the clade C isolates, 44.3%, 27.8%, 15.7% and 6.7% were in clades C2, C other, C1\u2010non\u2010M27 and C1\u2010M27, respectively. Approximately 11% of the ST131 isolates were carbapenem resistant. ExPEC from dogs showed comparable resistance rates to human isolates except for resistance to amoxicillin\u2010clavulanate and amikacin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We observed a high occurrence of ST131 and its clade C, with more than 11% exhibiting resistance to carbapenems in Sri Lanka. Furthermore, ST131\u2010C1\u2010M27, with high resistance to both quinolones and extended\u2010spectrum cephalosporins, was also present. Our results emphasise the importance of the One Health approach in the monitoring of antimicrobial\u2010resistant <jats:styled-content style=\"fixed-case\"><jats:italic>E.\u2009coli<\/jats:italic><\/jats:styled-content> in different regions of Sri Lanka to gain a better understanding of their prevalence over time, contributing to effective antimicrobial stewardship.<\/jats:p><\/jats:sec>","DOI":"10.1111\/zph.13199","type":"journal-article","created":{"date-parts":[[2024,12,6]],"date-time":"2024-12-06T04:40:11Z","timestamp":1733460011000},"page":"200-206","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["High Occurrence of <scp>ST131<\/scp> Among Extraintestinal Pathogenic <scp><i>Escherichia coli<\/i><\/scp> in Humans and Dogs in Sri Lanka"],"prefix":"10.1111","volume":"72","author":[{"given":"W. A. Pamudi Maldam","family":"Dewasmika","sequence":"first","affiliation":[{"name":"Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine and Animal Science University of Peradeniya Sri Lanka  Peradeniya Sri Lanka"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9902-2426","authenticated-orcid":false,"given":"D. R. Anuruddhika","family":"Dissanayake","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine and Animal Science University of Peradeniya Sri Lanka  Peradeniya Sri Lanka"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. G. Dulanjalee Anuruddhika Kumari","family":"Neelawala","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine and Animal Science University of Peradeniya Sri Lanka  Peradeniya Sri Lanka"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. G. Nilanthi","family":"Dissanayake","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Medicine University of Peradeniya  Peradeniya Sri Lanka"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. V. Pahan","family":"Prasada","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture University of Peradeniya  Peradeniya Sri Lanka"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veithehi Rajeevan","family":"Francis","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Faculty of Healthcare Sciences Eastern University  Batticaloa Sri Lanka"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Octavia","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Biomolecular Sciences University of new South Wales  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiting","family":"Lan","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Biomolecular Sciences University of new South Wales  Sydney New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1910.130309"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879\u2010019\u20104258\u2010y"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01260"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2004.02.015"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010019\u201049467\u20105"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp194"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/1469\u20100691.12028"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02562\u201014"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560\u20107917.ES.2019.24.37.1800627"},{"key":"e_1_2_9_11_1","volume-title":"EUCAST Guidelines for Detection of Resistance Mechanisms and Specific Resistances of Clinical and\/or Epidemiological Importance","author":"European Committee on Antimicrobial Susceptibility Testing","year":"2019"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2019.0312"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1086\/653932"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2020.108783"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2006.01.014"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2211.160519"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/aac.00179\u201017"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.736464"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkz304"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00125\u201013"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkab020"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40265\u2010019\u201001180\u20103"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.10609.1"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473\u20103099(08)70041\u20100"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiv429"},{"key":"e_1_2_9_26_1","volume-title":"A Language and Environment for Statistical Computing","author":"R Core Team","year":"2013"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joad.2015.07.007"},{"key":"e_1_2_9_28_1","volume-title":"Clinical and Laboratory Standards Institute: Performance Standards for Antimicrobial Susceptibility Testing: 20th Informational Supplement","author":"Wayne P. 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