{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T23:12:49Z","timestamp":1793661169159,"version":"4.5.1"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T00:00:00Z","timestamp":1736467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011073","name":"VetAgro Sup","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011073","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Leptospirosis is a widespread zoonosis caused by bacteria in the genus <jats:italic>Leptospira.<\/jats:italic> Basic epidemiological information is crucial to mitigating disease risk but is lacking for leptospirosis; notably, the hosts responsible for maintaining <jats:italic>Leptospira<\/jats:italic> remain largely unknown. Frequently observed near human habitations, hedgehogs (Erinaceus europaeus) are taken to wildlife rescue centres when found sick or injured. Thus, they may pose a risk to human and animal health if they carry pathogenic <jats:italic>Leptospira<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aims<\/jats:title><jats:p>This study aimed to describe <jats:italic>Leptospira<\/jats:italic> carriage in a hedgehog population and the potential clinical impacts of the infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Method<\/jats:title><jats:p>We investigated <jats:italic>Leptospira<\/jats:italic> carriage frequency and diversity in urine samples from 69 hedgehogs at a wildlife rescue centre, between April and June 2022. We used quantitative PCR, typing of the <jats:italic>16S rRNA<\/jats:italic> and <jats:italic>lfb1<\/jats:italic> genes, variable number tandem repeat and multispacer sequence typing to characterise <jats:italic>Leptospira<\/jats:italic> DNA. An analysis of urinary biochemical parameters was conducted to assess renal function.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We detected <jats:italic>Leptospira<\/jats:italic> DNA in 25 (35%) of the urine samples, of which 21 were successfully typed. The latter analysis revealed a limited degree of genetic diversity. <jats:italic>L. interrogan<\/jats:italic>s (<jats:italic>n<\/jats:italic>\u2009=\u200919) predominated, and the only genotype detected was related to the Australis serogroup (<jats:italic>n<\/jats:italic>\u2009=\u200917). We also noted the presence of <jats:italic>L. borgpetersenii<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2009=\u20091) and <jats:italic>L. kirschneri<\/jats:italic> (<jats:italic>n<\/jats:italic>\u2009=\u20091). There was no relationship between infection status and urinalysis parameters.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results suggest hedgehogs may act as long\u2010term shedders of <jats:italic>Leptospira<\/jats:italic> in natural ecosystems.<\/jats:p><\/jats:sec>","DOI":"10.1111\/zph.13206","type":"journal-article","created":{"date-parts":[[2025,1,11]],"date-time":"2025-01-11T07:19:24Z","timestamp":1736579964000},"page":"324-329","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Chronic Carriage of <scp><i>Leptospira interrogans<\/i><\/scp> Genotype Associated With the Australis Serogroup by Naturally\u2010Infected Hedgehogs (<scp><i>Erinaceus europaeus<\/i><\/scp>) at a Wildlife Health Centre in Northwestern France"],"prefix":"10.1111","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7119-3765","authenticated-orcid":false,"given":"Florence","family":"Ayral","sequence":"first","affiliation":[{"name":"UR 1233 VetAgro Sup, Universit\u00e9 de Lyon  Marcy L'Etoile France"},{"name":"VetAgro Sup Analysis Center VetAgro Sup, Universit\u00e9 de Lyon  Marcy L'Etoile France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Botman","sequence":"additional","affiliation":[{"name":"CHUV Oniris  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marine","family":"Le Guyader","sequence":"additional","affiliation":[{"name":"UR 1233 VetAgro Sup, Universit\u00e9 de Lyon  Marcy L'Etoile France"},{"name":"VetAgro Sup Analysis Center VetAgro Sup, Universit\u00e9 de Lyon  Marcy L'Etoile France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eve","family":"Ramery","sequence":"additional","affiliation":[{"name":"VetAgro Sup Analysis Center VetAgro Sup, Universit\u00e9 de Lyon  Marcy L'Etoile France"},{"name":"UPSP ICE VetAgro Sup, Universit\u00e9 de Lyon  Marcy L'Etoile France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Gourlay","sequence":"additional","affiliation":[{"name":"CHUV Oniris  Nantes France"},{"name":"BIOEPAR Oniris, INRAE  Nantes 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