{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T10:10:07Z","timestamp":1751364607195,"version":"3.41.0"},"reference-count":55,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T00:00:00Z","timestamp":1743984000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008323","name":"Zanjan University of Medical Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008323","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Cystic echinococcosis (CE) is a serious global health concern. CE is an endemic zoonotic disease in Iran. The substantial genetic diversity of <jats:italic>Echinococcus granulosus<\/jats:italic> is closely linked to its life cycle, transmission mechanisms and pathogenesis. This study aimed to explore the phylogenetic structure of the genotypes of <jats:styled-content style=\"fixed-case\"><jats:italic>E. granulosus<\/jats:italic><\/jats:styled-content> sensu lato (s.l.) in southwestern Iran (SWI).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Seventy\u2010two hydatid cyst samples were collected from various intermediate hosts, including goats, cattle, sheep, camels, buffalo and humans, primarily from the lungs, liver, spleen and heart of livestock slaughtered in local and industrial abattoirs, as well as from patients in several hospitals (Namazi, Golestan, Arvand and Shahid Beheshti) in southwestern Iran (Bushehr, Fars, Kohgiluyeh and Boyerahmad and Khuzestan provinces).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Sequence analysis revealed three genotypes of <jats:styled-content style=\"fixed-case\"><jats:italic>E. granulosus<\/jats:italic><\/jats:styled-content> s.l. in the SWI: G<jats:sub>1<\/jats:sub> (<jats:italic>n<\/jats:italic>\u2009=\u200969, 95.8%), G<jats:sub>3<\/jats:sub>(<jats:italic>n<\/jats:italic>\u2009=\u20091, 1.4%) and genotype like G<jats:sub>6<\/jats:sub>\/G<jats:sub>7<\/jats:sub> (<jats:italic>n<\/jats:italic>\u2009=\u20092, 2.8%). Twenty\u2010one different haplotypes were identified in this study. According to the phylogenetic tree topology, the <jats:italic>cox<\/jats:italic>1 gene sequence similarities can be shown for some G<jats:sub>1<\/jats:sub> and G<jats:sub>6<\/jats:sub>\/G<jats:sub>7<\/jats:sub> isolates in some vast areas, but these genotypes generally show a heterogeneous population worldwide. High similarity was shown between G<jats:sub>3<\/jats:sub> isolates from some countries (Spain, Turkey, India and Iran) and different hosts (sheep, human and buffalo).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The presence of the G<jats:sub>6<\/jats:sub>\/G<jats:sub>7<\/jats:sub> genotype in the SWI may be due to the transmission of this genotype from other regions or the role of camel\/wild boar and other possible intermediate hosts in the expansion of this genotype in the SWI. The results of the present study can be used in CE control programs, molecular epidemiology and phylogenetic studies in Iran and other countries for future goals.<\/jats:p><\/jats:sec>","DOI":"10.1111\/zph.13220","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T05:26:02Z","timestamp":1744089962000},"page":"435-441","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phylogenetic Study of <i>cox1<\/i> Gene in <i>Echinococcus granulosus<\/i> Sensu Lato Genotypes in Southwestern Iran"],"prefix":"10.1111","volume":"72","author":[{"given":"Ali","family":"Jamshidi","sequence":"first","affiliation":[{"name":"Department of Medical Parasitology and Mycology, School of Medicine Zanjan University of Medical Sciences  Zanjan Iran"},{"name":"Behbahan Faculty of Medical Sciences  Behbahan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Amin","family":"Ghatee","sequence":"additional","affiliation":[{"name":"Professor Alborzi Clinical Microbiology Research Center Shiraz University of Medical Sciences  Shiraz Iran"},{"name":"Department of Microbiology, School of Medicine Yasuj University of Medical Sciences  Yasuj Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Haniloo","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology and Mycology, School of Medicine Zanjan University of Medical Sciences  Zanjan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asghar","family":"Fazaeli","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology and Mycology, School of Medicine Zanjan University of Medical Sciences  Zanjan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamad","family":"Sabaghan","sequence":"additional","affiliation":[{"name":"Behbahan Faculty of Medical Sciences  Behbahan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,4,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.5455\/IJE.2023.01.01"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.4142\/jvs.22218"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2024.110273"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.5455\/OVJ.2024.v14.i3.14"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071\u2010020\u201004199\u20108"},{"issue":"2","key":"e_1_2_8_7_1","first-page":"140","article-title":"Phylogeography, Genetic Diversity and Population Structure of Echinococcus granulosus Sensu Stricto Inferred by Mitochondrial DNA Markers Between Southeast of Iran and Pakistan","volume":"19","author":"Anvari D.","year":"2024","journal-title":"Iranian Journal of Parasitology"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1141192"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-019-06391-x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(92)90109-W"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(93)90065-7"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X18000883"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-022-05197-8"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2020.109132"},{"key":"e_1_2_8_15_1","article-title":"Meta\u2010Analysis of the Prevalence of Echinococcus in Sheep in China From 1983 to 2020","volume":"2","author":"Cong W.","year":"2022","journal-title":"Advances in Diagnosis and Therapeutic Intervention for Foodborne Parasitic Diseases"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/tmi.12647"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2015.09.029"},{"issue":"71","key":"e_1_2_8_18_1","first-page":"60","article-title":"Genetic Variation of Echinococcus granulosus Isolates From Indian Buffalo and Iranian Sheep, Cattle and Camel","volume":"19","author":"Gholami S.","year":"2009","journal-title":"Journal of Mazandaran University of Medical Sciences"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917\u2010021\u201003131\u20101"},{"issue":"84","key":"e_1_2_8_20_1","first-page":"57","article-title":"Genotype Characterization of Hydatid Cysts Isolated From Zanjan Using PCR\u2010RFLP Technique","volume":"21","author":"Haniloo A.","year":"2013","journal-title":"Journal of Advances in Medical and Biomedical Research"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vprsr.2021.100655"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.7589\/2018-09-215"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.4236\/ojvm.2012.24032"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.14469"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X17000967"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X20000553"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.59779\/jiomnepal.524"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2017.08.018"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X18000275"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2018.03.006"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2018.06.026"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182018000549"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182013001340"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2015.07.031"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2019.103941"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182002002792"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apar.2016.07.002"},{"key":"e_1_2_8_38_1","first-page":"1","article-title":"Echinococcus granulosus Sensu Lato Genotypes in Different Hosts Worldwide: A Systematic Review","volume":"67","author":"Manterola C.","year":"2021","journal-title":"Acta Parasitologica"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-021-07186-9"},{"issue":"1","key":"e_1_2_8_40_1","first-page":"59","article-title":"Establishment of a Modified In\u00a0Vitro Cultivation of Protoscoleces to Adult Echinococcus granulosus; an Important Way for New Investigations on Hydatidosis","volume":"7","author":"Mohammadzadeh T.","year":"2012","journal-title":"Iranian Journal of Parasitology"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0020\u20107519(03)00132\u20102"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1080\/15321819.2018.1547974"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2023.106951"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-019-3644-z"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2015.07.035"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apar.2016.11.002"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2013.06.008"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.17420\/ap6104.18"},{"issue":"1","key":"e_1_2_8_49_1","first-page":"1","article-title":"The Random Amplification of Polymorphic DNA Can Discriminate Species and Strains of Echinococcus","volume":"45","author":"Scott J.","year":"1994","journal-title":"Tropical Medicine and Parasitology"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/ccr3.6095"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182022000658"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200900145"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436\u2010009\u20101376\u20102"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1186\/2049-9957-3-3"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2012.05.014"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(99)90043-3"}],"container-title":["Zoonoses and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/zph.13220","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T09:28:32Z","timestamp":1751362112000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/zph.13220"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,7]]},"references-count":55,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["10.1111\/zph.13220"],"URL":"https:\/\/doi.org\/10.1111\/zph.13220","archive":["Portico"],"relation":{},"ISSN":["1863-1959","1863-2378"],"issn-type":[{"type":"print","value":"1863-1959"},{"type":"electronic","value":"1863-2378"}],"subject":[],"published":{"date-parts":[[2025,4,7]]},"assertion":[{"value":"2024-04-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}