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This study aims to characterise novel class 1 integron (Int1) structures containing bla<\/jats:italic>VIM\u20102<\/jats:sub> and bla<\/jats:italic>IMP\u20101<\/jats:sub> in high\u2010risk CP\u2010Pa<\/jats:italic> sequence type (ST) 235 and ST654 strains from dogs and cats and illustrate the genetic relatedness between CP\u2010Pa<\/jats:italic> strains from animal and human origins.<\/jats:p><\/jats:sec>Methods and Results<\/jats:title>Of the four CP\u2010Pa<\/jats:italic> strains, whole\u2010genome sequencing and analysis revealed that three strains belonged to ST235\/O11\/exoU+<\/jats:italic>\/exoS\u2010<\/jats:italic>, with two strains harbouring bla<\/jats:italic>VIM\u20102<\/jats:sub>, and one strain harbouring bla<\/jats:italic>IMP\u20101<\/jats:sub>. The remaining strain was characterised as ST654\/O4\/exoU\u2010<\/jats:italic>\/exoS+<\/jats:italic> and harboured bla<\/jats:italic>VIM\u20102<\/jats:sub>. Core\u2010genome single nucleotide polymorphism\u2010based phylogeny illustrated genetic relationships between animal and human high\u2010risk CP\u2010Pa<\/jats:italic> ST235\/O11 and ST654\/O4 strains within the same STs. Three novel Int1 gene cassette arrays were identified in the canine and feline CP\u2010Pa<\/jats:italic> strains in this study. The novel ~6.2\u2010kb bla<\/jats:italic>VIM\u20102<\/jats:sub>\u2010containing Int1[Pae\u2010CUVET21\u2010397] and Int1[Pae\u2010CUVET23\u2010830] (intI1<\/jats:italic>\u2010aadB<\/jats:italic>\u2010bla<\/jats:italic>VIM\u20102<\/jats:sub>\u2010aadB<\/jats:italic>\u2010cmlA6<\/jats:italic>\u2010qacE<\/jats:italic>\u22061<\/jats:italic>\u2010folP<\/jats:italic>) formed a complex Int1 located in an integrative and conjugative element of two feline CP\u2010Pa<\/jats:italic> ST235 strains. The canine CP\u2010Pa<\/jats:italic> ST235 strain CUVET20\u2010956 contained a novel ~5.8\u2010kb bla<\/jats:italic>IMP\u20101<\/jats:sub>\u2010containing Int1[Pae\u2010CUVET20\u2010956] (intI1<\/jats:italic>\u2010bla<\/jats:italic>IMP\u20101<\/jats:sub>\u2010aacA4<\/jats:italic>\u2010aacA4<\/jats:italic>\u2010IS1595<\/jats:italic>\u2010qacE<\/jats:italic>\u22061<\/jats:italic>\u2010sul1<\/jats:italic>). The CP\u2010Pa<\/jats:italic> ST654 strain CUVET18\u2010860 contained In1206<\/jats:italic> encoding bla<\/jats:italic>VIM\u20102<\/jats:sub> and a novel ~5.9\u2010kb Int1[Pae\u2010CUVET18\u2010860] (\u2206intI1<\/jats:italic>\u2010bla<\/jats:italic>VEB\u20101<\/jats:sub>\u2010aadB<\/jats:italic>\u2010qnrVC1<\/jats:italic>\u2010aacA4<\/jats:italic>\u2010bla<\/jats:italic>OXA\u201010<\/jats:sub>\u2010aadA1<\/jats:italic>\u2010dfrA14<\/jats:italic>) encoding multidrug resistance (MDR). Additionally, In51<\/jats:italic>\u2010containing Tn6162<\/jats:italic> inserted in genomic island 1 was identified in all CP\u2010Pa<\/jats:italic> ST235 strains.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The novel Int1s encoding metallo\u2010\u03b2\u2010lactamases and MDR in canine and feline CP\u2010Pa<\/jats:italic> play a crucial role in the development of resistance to multiple antimicrobials and last\u2010resort carbapenems in P. aeruginosa<\/jats:italic><\/jats:styled-content> ST235 and ST654 strains of veterinary and public health importance, posing possible zoonotic and anthroponotic transmission of the high\u2010risk CP\u2010Pa<\/jats:italic> clones between companion animals and humans.<\/jats:p><\/jats:sec>","DOI":"10.1111\/zph.13224","type":"journal-article","created":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T01:15:27Z","timestamp":1746407727000},"page":"453-464","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Novel Class 1 Integron Structures in High\u2010Risk Metallo\u2010\u03b2\u2010Lactamase\u2010Producing Pseudomonas aeruginosa<\/i> ST235 and ST654 From Companion Animals"],"prefix":"10.1111","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7835-2191","authenticated-orcid":false,"given":"Chavin","family":"Leelapsawas","sequence":"first","affiliation":[{"name":"Department of Veterinary Microbiology, Faculty of Veterinary Science Chulalongkorn University Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rungtip","family":"Chuanchuen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health, Faculty 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