{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T09:27:22Z","timestamp":1770974842473,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T00:00:00Z","timestamp":1764720000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T00:00:00Z","timestamp":1764720000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC2307301"],"award-info":[{"award-number":["2023YFC2307301"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2026,3]]},"abstract":"ABSTRACT<\/jats:title>\n \n Introduction<\/jats:title>\n \n Bovine tuberculosis (bTB) has a certain incidence on farms in China, but little is known about its prevalence and the pathogenic\n Mycobacterium<\/jats:italic>\n species. With the increasing application of whole\u2010genome sequencing (WGS), it is now possible to investigate the genomic diversity, lineage distribution, and drug resistance profiles of\n \n M. bovis<\/jats:italic>\n <\/jats:styled-content>\n at high resolution.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n In this study, we sequenced 29\n \n Mycobacterium tuberculosis<\/jats:italic>\n <\/jats:styled-content>\n complex (MTBC) isolates from two farms in China and incorporated genomic data from 79 publicly available\n \n M. bovis<\/jats:italic>\n <\/jats:styled-content>\n isolates from cattle farms in China for a comprehensive genomic analysis.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The majority of the 106\n \n M. bovis<\/jats:italic>\n <\/jats:styled-content>\n isolates (63.2%, 67\/106) belong to lineage La1.4. The dataset was screened for isolates of two known clonal complexes, Eu1 and Eu3, corresponding to La1.8.1 and La1.2, respectively. In silico spoligotyping revealed nine previously identified profiles, and the most common spoligotype in this study is SB1903. All isolates have the H57D mutation in the\n pncA<\/jats:italic>\n gene associated with pyrazinamide (PZA) resistance. 16.0% (17\/106) of isolates have resistance mutations to first\u2010 or second\u2010line drugs other than PZA, with one isolate classified as pre\u2010XDR\u2010TB. The distribution of cluster strains suggests the possibility of recent transmission and outbreak in the two farms. It is noted that two\n \n M. tuberculosis<\/jats:italic>\n <\/jats:styled-content>\n isolates from tissue samples had detectable drug resistance mutations.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n The results of this study provide the basis for in\u2010depth analysis of genomic population characteristics and drug resistance of\n \n M. bovis<\/jats:italic>\n <\/jats:styled-content>\n in China. It should be noted that close contact between humans and cattle increases the risk of\n \n M. tuberculosis<\/jats:italic>\n <\/jats:styled-content>\n transmission from humans to cattle in rural areas or developing countries with low levels of human\u2010animal separation.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/zph.70026","type":"journal-article","created":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T04:49:51Z","timestamp":1764823791000},"page":"128-136","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Whole Genome Sequencing Analysis Revealed High Genetic Diversity and Drug\u2010Resistant Characteristics of\n Mycobacterium bovis<\/i>\n From Chinese Farms"],"prefix":"10.1111","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-6093-1548","authenticated-orcid":false,"given":"Xinyue","family":"Li","sequence":"first","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Center for Tuberculosis Control and Prevention Chinese Centre for Disease Control and Prevention Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2891-8449","authenticated-orcid":false,"given":"Yuhui","family":"Dong","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine China Agricultural University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruida","family":"Xing","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Center for Tuberculosis Control and Prevention Chinese Centre for Disease Control and Prevention Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Pathology Capital Medical University Affiliated Beijing Chest Hospital Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Zhao","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Center for Tuberculosis Control and Prevention Chinese Centre for Disease Control and Prevention Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaojun","family":"Pei","sequence":"additional","affiliation":[{"name":"School of Public Health Peking University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaolong","family":"Cao","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Center for Tuberculosis Control and Prevention Chinese Centre for Disease Control and Prevention Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruiqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Center for Tuberculosis Control and Prevention Chinese Centre for Disease Control and Prevention Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiangyong","family":"Zeng","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine Tibet Academy of Agriculture and Animal Husbandry Sciences Lhasa Tibet China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongjing","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Animal Husbandry and Veterinary Medicine Tibet Academy of Agriculture and Animal Husbandry Sciences Lhasa Tibet China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weixing","family":"Fan","sequence":"additional","affiliation":[{"name":"National Animal Tuberculosis Reference Laboratory, Division of Zoonoses Surveillance China Animal Health and Epidemiology Center Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Xia","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Center for Tuberculosis Control and Prevention Chinese Centre for Disease Control and Prevention Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengfen","family":"Wang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Center for Tuberculosis Control and Prevention Chinese Centre for Disease Control and Prevention Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3126-4450","authenticated-orcid":false,"given":"Xichao","family":"Ou","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Center for Tuberculosis Control and Prevention Chinese Centre for Disease Control and Prevention Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanlin","family":"Zhao","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Center for Tuberculosis Control and Prevention Chinese Centre for Disease Control and Prevention Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,3]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"539","article-title":"Population Structure and Transmission of Mycobacterium bovis in Ethiopia","volume":"5","author":"Almaw G.","year":"2021","journal-title":"Microbial Genomics"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00750-10"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens11070715"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11010177"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2008.07.003"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-015-0164-0"},{"key":"e_1_2_11_8_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0008894","article-title":"Broad Diversity of Mycobacterium tuberculosis Complex Strains Isolated From Humans and Cattle in Northern Algeria Suggests a Zoonotic Transmission Cycle","volume":"11","author":"Damene H.","year":"2020","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2704.204467"},{"key":"e_1_2_11_10_1","first-page":"e2769","article-title":"Genomic Analysis of Diversity, Biogeography, and Drug Resistance in Mycobacterium bovis","volume":"5","author":"Dong Y.","year":"2022","journal-title":"Transboundary and Emerging Diseases"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2022.1006090"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-016-3213-1"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2017.02.006"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-26248-1"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2018.03.036"},{"key":"e_1_2_11_16_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0009502","article-title":"Prevalence of Bovine Tuberculosis in Dairy Cattle in China During 2010\u20132019: A Systematic Review and Meta\u2010Analysis","volume":"6","author":"Gong Q. L.","year":"2021","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-017-4249-6"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/emph\/eoaa005"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01590-08"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-54268-x"},{"key":"e_1_2_11_21_1","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.03829-23","article-title":"Population Structure and History of Mycobacterium bovis European 3 Clonal Complex Reveal Transmission Across Ecological Corridors of Unrecognized Importance in Portugal","volume":"7","author":"Pereira A. C.","year":"2024","journal-title":"Microbiology Spectrum"},{"key":"e_1_2_11_22_1","article-title":"The Open Pan\u2010Genome Architecture and Virulence Landscape of Mycobacterium bovis","volume":"10","author":"Reis A. C.","year":"2021","journal-title":"Microbial Genomics"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2011.09.004"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2024.1345328"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2011.04.027"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2011.08.002"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-024-10909-8"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.6.2996-2997.2005"},{"key":"e_1_2_11_29_1","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.01842-23","article-title":"Association of Lineage 4.2.2 of Mycobacterium tuberculosis With the 63\u2010bp Deletion Variant of the mpt64 Gene","volume":"6","author":"Song Z.","year":"2023","journal-title":"Microbiology Spectrum"},{"key":"e_1_2_11_30_1","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.01324-23","article-title":"Insight Into the Drug\u2010Resistant Characteristics and Genetic Diversity of Multidrug\u2010Resistant Mycobacterium tuberculosis in China","volume":"5","author":"Song Z.","year":"2023","journal-title":"Microbiology Spectrum"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2011.03.012"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1412.070727"},{"key":"e_1_2_11_33_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0009145","article-title":"Identification of Drug Resistance Mutations Among Mycobacterium bovis Lineages in the Americas","volume":"2","author":"V\u00e1zquez\u2010Chac\u00f3n C. A.","year":"2021","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(21)00301-3"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-019-1891-0"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13073-016-0270-7"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad359"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2023.105887"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02389"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00843"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.12688\/openreseurope.14029.2"}],"container-title":["Zoonoses and Public Health"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/zph.70026","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/zph.70026","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/zph.70026","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T08:01:04Z","timestamp":1770969664000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/zph.70026"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,3]]},"references-count":40,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1111\/zph.70026"],"URL":"https:\/\/doi.org\/10.1111\/zph.70026","archive":["Portico"],"relation":{},"ISSN":["1863-1959","1863-2378"],"issn-type":[{"value":"1863-1959","type":"print"},{"value":"1863-2378","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,3]]},"assertion":[{"value":"2025-04-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}