{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:04:04Z","timestamp":1789427044342,"version":"build-2803163510"},"reference-count":90,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T00:00:00Z","timestamp":1764374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["NU21\u201005\u201000143"],"award-info":[{"award-number":["NU21\u201005\u201000143"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2026,2]]},"abstract":"ABSTRACT<\/jats:title>\n \n Introduction<\/jats:title>\n Black flies (Simuliidae) are globally distributed blood\u2010feeding arthropods and vectors of viral, bacterial, and parasitic pathogens to many animal species, including humans. We investigated the occurrence of selected vector\u2010borne pathogens in black flies in South Moravia, Czech Republic, and evaluated their possible role in the circulation of vector\u2010borne pathogens.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n A total of 11,600 black flies comprising four species of the genus\n Simulium<\/jats:italic>\n , namely\n Simulium<\/jats:italic>\n (\n Boophthora<\/jats:italic>\n )\n erythrocephalum<\/jats:italic>\n (De Geer, 1776),\n Simulium<\/jats:italic>\n (\n Wilhelmia<\/jats:italic>\n )\n lineatum<\/jats:italic>\n (Meigen, 1804),\n Simulium<\/jats:italic>\n (\n Wilhelmia<\/jats:italic>\n )\n balcanicum<\/jats:italic>\n (Enderlein, 1924), and\n Simulium<\/jats:italic>\n (\n Wilhelmia<\/jats:italic>\n )\n turgaicum<\/jats:italic>\n (Rubtsov, 1940) were pooled and screened for the following arthropod\u2010borne pathogens and parasites endemic in Central Europe: viruses (alphaviruses, bunyaviruses and flaviviruses), bacteria (\n \n Borrelia burgdorferi<\/jats:italic>\n <\/jats:styled-content>\n sensu lato,\n \n Borrelia miyamotoi<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n Anaplasma phagocytophilum<\/jats:italic>\n <\/jats:styled-content>\n ,\n Neoehrlichia mikurensis<\/jats:italic>\n ,\n Bartonella<\/jats:italic>\n spp.,\n Rickettsia<\/jats:italic>\n spp.,\n \n Francisella tularensis<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n Coxiella burnetii<\/jats:italic>\n <\/jats:styled-content>\n , and\n Brucella<\/jats:italic>\n spp.), protista (\n Babesia<\/jats:italic>\n spp.,\n Encephalitozoon<\/jats:italic>\n spp. and\n Enterocytozoon<\/jats:italic>\n spp.) and filaria (\n Dirofilaria<\/jats:italic>\n spp.,\n Setaria<\/jats:italic>\n spp., and\n Onchocerca<\/jats:italic>\n spp.).\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Almost all pools were negative for known arthropod\u2010borne pathogens and parasites. However, four new\n Bartonella<\/jats:italic>\n spp. variants were found that share similarity with other bartonellae reported from diverse arthropods and humans. The phylogenetic analysis of\n Bartonella<\/jats:italic>\n sequences from Czech black flies provides further evidence about an expanding diversity of\n Bartonella<\/jats:italic>\n lineages in arthropods globally, including hematophagous species (e.g., ticks, mosquitoes, and biting flies) and non\u2010hematophagous species (e.g., bees and ants). These bartonellae have the potential to cause pathogenic infections in humans who are exposed to arthropods carrying these bacteria.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n Summing up, this study provides for the very first time valuable data for characterising the risk to public and veterinary health from black flies and the infections they may carry in Europe. Further testing, however, should include a wider geographic, seasonal, and taxonomic range of black flies.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/zph.70031","type":"journal-article","created":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T14:24:44Z","timestamp":1764426284000},"page":"95-106","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Molecular Screening of Black Flies (Diptera: Simuliidae) for Vector\u2010Borne Zoonotic Pathogens, South Moravia, Czech Republic"],"prefix":"10.1111","volume":"73","author":[{"given":"Silvie","family":"\u0160ikutov\u00e1","sequence":"first","affiliation":[{"name":"Institute of Vertebrate Biology Czech Academy of Sciences Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krist\u00edna","family":"Mravcov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Vertebrate Biology Czech Academy of Sciences Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Mendel","sequence":"additional","affiliation":[{"name":"Institute of Vertebrate Biology Czech Academy of Sciences Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Old\u0159ich","family":"\u0160ebesta","sequence":"additional","affiliation":[{"name":"Institute of Vertebrate Biology Czech Academy of Sciences Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bohumil","family":"Sak","sequence":"additional","affiliation":[{"name":"Biology Centre, Institute of Parasitology Czech Academy of Sciences \u010cesk\u00e9 Bud\u011bjovice Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikola","family":"Holubov\u00e1","sequence":"additional","affiliation":[{"name":"Biology Centre, Institute of Parasitology Czech Academy of Sciences \u010cesk\u00e9 Bud\u011bjovice Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Kv\u00e1\u010d","sequence":"additional","affiliation":[{"name":"Biology Centre, Institute of Parasitology Czech Academy of Sciences \u010cesk\u00e9 Bud\u011bjovice Czech Republic"},{"name":"Faculty of Agriculture and Technology University of South Bohemia in \u010cesk\u00e9 Bud\u011bjovice \u010cesk\u00e9 Bud\u011bjovice Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6149-0598","authenticated-orcid":false,"given":"Clifton","family":"McKee","sequence":"additional","affiliation":[{"name":"Department of Epidemiology Johns Hopkins Bloomberg School of Public Health Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6772-8280","authenticated-orcid":false,"given":"Peter H.","family":"Adler","sequence":"additional","affiliation":[{"name":"Department of Plant and Environmental Sciences Clemson University Clemson South Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7518-476X","authenticated-orcid":false,"given":"D.","family":"Otranto","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine University of Bari Bari Italy"},{"name":"Department of Veterinary Clinical Sciences City University of Hong Kong Hong Kong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6602-2905","authenticated-orcid":false,"given":"Ivo","family":"Rudolf","sequence":"additional","affiliation":[{"name":"Institute of Vertebrate Biology Czech Academy of Sciences Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,29]]},"reference":[{"key":"e_1_2_12_2_1","first-page":"127","volume-title":"Biology of Disease Vectors","author":"Adler P. 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