{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T21:33:09Z","timestamp":1790371989913,"version":"4.1.0"},"reference-count":71,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T00:00:00Z","timestamp":1771027200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T00:00:00Z","timestamp":1771027200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100020884","name":"Agencia Nacional de Investigaci\u00f3n y Desarrollo","doi-asserted-by":"publisher","award":["11231174"],"award-info":[{"award-number":["11231174"]}],"id":[{"id":"10.13039\/501100020884","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2026,5]]},"abstract":"ABSTRACT<\/jats:title>\n \n Introduction<\/jats:title>\n \n The emergence of methicillin\u2010resistant\n Staphylococcus<\/jats:italic>\n species (MRS) poses a growing threat to both human and veterinary health.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n We performed a comparative genomic characterisation of 49 methicillin\u2010resistant\n Staphylococcus<\/jats:italic>\n isolates collected from humans, dogs, and cats in a veterinary hospital from Central Chile.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Whole\u2010genome sequencing revealed\n \n S. aureus<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n S. pseudintermedius<\/jats:italic>\n <\/jats:styled-content>\n , and\n \n S. epidermidis<\/jats:italic>\n <\/jats:styled-content>\n as the most prevalent species, with distinct patterns of antimicrobial resistance and virulence. Average Nucleotide Identity (ANI) and phylogenetic analyses highlighted species\u2010specific clustering but also revealed divergent lineages among\n \n Staphylococcus aureus<\/jats:italic>\n <\/jats:styled-content>\n and\n \n Staphylococcus epidermidis<\/jats:italic>\n <\/jats:styled-content>\n isolates. Resistance to \u03b2\u2010lactams was widespread, with the\n blaZ<\/jats:italic>\n and\n mecA<\/jats:italic>\n genes predominating, while macrolide and aminoglycoside resistance genes were variably distributed.\n \n S. aureus<\/jats:italic>\n <\/jats:styled-content>\n exhibited the highest virulence gene content, whereas\n \n S. pseudintermedius<\/jats:italic>\n <\/jats:styled-content>\n isolates carried superantigen\u2010encoding enterotoxin genes, suggesting potential zoonotic risk. MLST and SCCmec typing confirmed the circulation of community\u2010associated MRS lineages, notably SCCmec type IV.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n This study provides a first comprehensive characterisation of methicillin\u2010resistant\n Staphylococcus<\/jats:italic>\n spp. isolates from human and animal hosts at a veterinary hospital in Santiago, Chile. These findings underscore the need for continuous surveillance under the One Health approach to monitor the potential transmission of high\u2010risk clones and resistance determinants between humans and companion animals.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/zph.70042","type":"journal-article","created":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T09:20:56Z","timestamp":1771060856000},"page":"205-217","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["One Health Genomic Analysis of Methicillin\u2010Resistant\n \n Staphylococcus<\/i>\n spp.\n <\/scp>\n From Humans, Cats, and Dogs in a Veterinary Hospital in Central Chile"],"prefix":"10.1111","volume":"73","author":[{"given":"Daniel","family":"Garrido","sequence":"first","affiliation":[{"name":"Departamento de Ingenier\u00eda Qu\u00edmica y Bioprocesos Pontificia Universidad Cat\u00f3lica Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2845-4330","authenticated-orcid":false,"given":"Danny","family":"Fuentes\u2010Castillo","sequence":"additional","affiliation":[{"name":"Departamento de Patolog\u00eda y Medicina Preventiva, Facultad de Ciencias Veterinarias Universidad de Concepci\u00f3n Chill\u00e1n Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslye Camila","family":"del R\u00edo","sequence":"additional","affiliation":[{"name":"Escuela de Medicina Veterinaria, Facultad de Ciencias de la Vida Universidad Andr\u00e9s Bello Santiago Chile"},{"name":"One Health Institute, Faculty of Life Sciences Universidad Andres Bello Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Barraza\u2010Schulmeyer","sequence":"additional","affiliation":[{"name":"Departamento de Patolog\u00eda y Medicina Preventiva, Facultad de Ciencias Veterinarias Universidad de Concepci\u00f3n Chill\u00e1n Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Melis","sequence":"additional","affiliation":[{"name":"Departamento de Ingenier\u00eda Qu\u00edmica y Bioprocesos Pontificia Universidad Cat\u00f3lica Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Departamento de Laboratorios Cl\u00ednicos, Facultad de Medicina Pontificia Universidad Cat\u00f3lica Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Flores\u2010Y\u00e1\u00f1ez","sequence":"additional","affiliation":[{"name":"Escuela de Medicina Veterinaria, Facultad de Ciencias de la Vida Universidad Andr\u00e9s Bello Santiago Chile"},{"name":"One Health Institute, Faculty of Life Sciences Universidad Andres Bello Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0860-627X","authenticated-orcid":false,"given":"Pamela","family":"Thomson","sequence":"additional","affiliation":[{"name":"Escuela de Medicina Veterinaria, Facultad de Ciencias de la Vida Universidad Andr\u00e9s Bello Santiago Chile"},{"name":"One Health Institute, Faculty of Life Sciences Universidad Andres Bello Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,14]]},"reference":[{"issue":"4","key":"e_1_2_10_2_1","first-page":"308","article-title":"Genotypic and Phenotypic Characterization of Methicillin Resistance Determinants and \u03b2\u2010Lactamase in Staphylococcus Species","volume":"13","author":"Abdul Rachman A. 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