{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T17:07:54Z","timestamp":1779124074632,"version":"3.51.4"},"reference-count":51,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Blastocystis<\/jats:italic>\n                      sp. is a cosmopolitan protist that colonises the gastrointestinal tract of mammals, including humans, dogs and cats. Although its pathogenicity remains debated,\n                      <jats:italic>Blastocystis<\/jats:italic>\n                      sp. has been linked to gastrointestinal symptoms and alterations in the gut microbiota. In Gabon, most epidemiological data on\n                      <jats:italic>Blastocystis<\/jats:italic>\n                      sp. rely on microscopy, which may underestimate true prevalence. Moreover, molecular studies remain scarce, and none have investigated the parasite's presence in domestic animals. This study aimed to estimate the prevalence and subtype (ST) distribution of\n                      <jats:italic>Blastocystis<\/jats:italic>\n                      sp. in humans, dogs and cats across three provinces of Gabon, within a One Health framework.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Between June 2021 and November 2022, a total of 182 faecal samples were collected: 127 from children in the Mulundu department (Ogoou\u00e9\u2010Lolo), 55 from both dogs and cats in Libreville (Estuaire) and Franceville (Haut\u2010Ogoou\u00e9). Samples were screened for the presence of\n                      <jats:italic>Blastocystis<\/jats:italic>\n                      sp. using conventional PCR targeting the SSU rRNA gene, positive isolates were subtyped.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Blastocystis<\/jats:italic>\n                      sp. was detected in 58.3% of human samples, with a significant association between infection and place of residence (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.02, fisher's exact test). Among animals, the parasite was found in 18.2% (8\/44) of dogs and 36.4% (4\/11) of cats. Subtyping revealed ST1 and ST3 in humans, while only ST1 was found in dogs. No subtypes were resolved in cats. These findings confirm the high circulation of\n                      <jats:italic>Blastocystis<\/jats:italic>\n                      sp. in both humans and domestic animals in Gabon and highlight the potential for zoonotic transmission.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This study provides the first molecular evidence of\n                      <jats:italic>Blastocystis<\/jats:italic>\n                      sp. infection in animals in Gabon and underscores the need for integrated surveillance strategies to better understand its epidemiology.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/zph.70044","type":"journal-article","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T04:11:23Z","timestamp":1771560683000},"page":"226-233","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Prevalence and Genetic Diversity of\n                    <i>Blastocystis<\/i>\n                    sp. in Humans, Dogs and Cats in Gabon: A One Health Perspective"],"prefix":"10.1111","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0192-5972","authenticated-orcid":false,"given":"P.","family":"Makouloutou\u2010Nzassi","sequence":"first","affiliation":[{"name":"D\u00e9partement de Biologie et Ecologie Animale Institut de Recherche en Ecologie Tropicale (IRET\/CENAREST)  Libreville Gabon"},{"name":"Unit\u00e9 de Recherche en Ecologie de la Sant\u00e9, Centre Interdisciplinaire de Recherches M\u00e9dicales de Franceville  Franceville Gabon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. M.","family":"Longo\u2010Pendy","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche en Ecologie de la Sant\u00e9, Centre Interdisciplinaire de Recherches M\u00e9dicales de Franceville  Franceville Gabon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. Ndong","family":"Atome","sequence":"additional","affiliation":[{"name":"Department of Biochimie, Faculty of Science Masuku University of Science and Technology (USTM)  Franceville Gabon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Mpassy","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche en Ecologie de la Sant\u00e9, Centre Interdisciplinaire de Recherches M\u00e9dicales de Franceville  Franceville Gabon"},{"name":"Department of Biochimie, Faculty of Science Masuku University of Science and Technology (USTM)  Franceville Gabon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Bangueboussa","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche en Ecologie de la Sant\u00e9, Centre Interdisciplinaire de Recherches M\u00e9dicales de Franceville  Franceville Gabon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. C.","family":"Atiga","sequence":"additional","affiliation":[{"name":"Unit of Evolution, Epidemiology and Parasite Resistance (UNEEREP) Franceville Interdisciplinary Center for Medical Research (CIRMF)  Franceville Gabon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. P.","family":"Okouga","sequence":"additional","affiliation":[{"name":"Unit of Evolution, Epidemiology and Parasite Resistance (UNEEREP) Franceville Interdisciplinary Center for Medical Research (CIRMF)  Franceville Gabon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. B.","family":"N'dilimabaka","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science Masuku University of Science and Technology (USTM)  Franceville Gabon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. L.","family":"Oyegue\u2010Liabagui","sequence":"additional","affiliation":[{"name":"Unit of Evolution, Epidemiology and Parasite Resistance (UNEEREP) Franceville Interdisciplinary Center for Medical Research (CIRMF)  Franceville Gabon"},{"name":"Department of Biology, Faculty of Science Masuku University of Science and Technology (USTM)  Franceville Gabon"},{"name":"Central African Regional Doctoral School in Tropical Infectiology (ECODRAC)  Franceville Gabon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. B.","family":"Lekana\u2010Douki","sequence":"additional","affiliation":[{"name":"Department of Biology, Faculty of Science Masuku University of Science and Technology (USTM)  Franceville Gabon"},{"name":"Department of Parasitology\u2010Mycology University of Health Sciences (USS)  Libreville Gabon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9551-3017","authenticated-orcid":false,"given":"L.","family":"Boundenga","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche en Ecologie de la Sant\u00e9, Centre Interdisciplinaire de Recherches M\u00e9dicales de Franceville  Franceville Gabon"},{"name":"Department of Anthropology Durham University  Durham UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,19]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2012.12.011"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.protis.2013.05.003"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep25255"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens14080773"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/vms3.70054"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2024.106876"},{"issue":"4","key":"e_1_2_13_8_1","first-page":"613","article-title":"Etude Epid\u00e9miologique des Parasitoses Intestinales Humaines au Niveau du C.H.U. d'Oran (Alg\u00e9rie)","volume":"2","author":"Benouis A.","year":"2013","journal-title":"International Journal of Innovation and Applied Studies"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.15537\/smj.2015.9.12444"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756\u20103305\u20101\u201040"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2021.104807"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0169659"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096\u2010017\u20103053\u20101"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1590\/s1678\u20109946202062051"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.4314\/ahs.v20i3.21"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009253"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2021.3149\u20103155"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102334\u201014\u2010164"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.533528"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed9020038"},{"key":"e_1_2_13_21_1","volume-title":"New Algorithms and Methods to Estimate Maximum\u2010Likelihood Phylogenies: Assessing the Performance of PhyML 3.0 | Systematic Biology","author":"Guindon S.","year":"2010"},{"issue":"41","key":"e_1_2_13_22_1","first-page":"95","article-title":"BioEdit: A User\u2010Friendly Biological Sequence Alignment Editor and Analysis Program for Windows 95\/98\/NT","volume":"41","author":"Hall T. 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