{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T13:48:04Z","timestamp":1787060884598,"version":"build-2736575974"},"reference-count":74,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T00:00:00Z","timestamp":1772668800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T00:00:00Z","timestamp":1772668800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Rickettsioses are predominantly zoonotic infections that circulate among animal populations and can be transmitted to humans, representing emerging vector\u2010borne diseases spread through infected arthropod vectors.\n                      <jats:italic>Rickettsia asembonensis<\/jats:italic>\n                      displays a broad geographic distribution and is predominantly associated with fleas. Recent reports have identified\n                      <jats:italic>R<\/jats:italic>\n                      .\n                      <jats:italic>asembonensis<\/jats:italic>\n                      in febrile human patients in Malaysia, Peru, and Zambia, underscoring its emerging zoonotic potential. In Thailand, however, investigations of\n                      <jats:italic>Rickettsia<\/jats:italic>\n                      spp. have concentrated mainly on ticks and fleas, with comparatively little information regarding infections in pet dogs and cats.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The present study examined\n                      <jats:italic>Rickettsia<\/jats:italic>\n                      spp. in 968 pets, 472 client\u2010owned dogs, and 496 client\u2010owned cats from the Bangkok and Nakhon Pathom regions, using the\n                      <jats:italic>17\u2010kDa<\/jats:italic>\n                      gene as a molecular marker. Genetic analyses of\n                      <jats:italic>Rickettsia<\/jats:italic>\n                      species were performed using maximum\u2010likelihood phylogeny with IQ\u2010TREE, Bayesian inference, pairwise sequence similarity assessment, and nucleotide sequence type network analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 114 pets tested positive, corresponding to a prevalence of 11.8%, with 21.6% (102\/472) in dogs and 2.4% (12\/496) in cats. Of these, 48 samples were sequenced, leading to the identification of\n                      <jats:italic>R<\/jats:italic>\n                      .\n                      <jats:italic>asembonensis<\/jats:italic>\n                      and either uncultured\n                      <jats:italic>Rickettsia<\/jats:italic>\n                      spp. or the\n                      <jats:italic>Rickettsia<\/jats:italic>\n                      endosymbiont of\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Haemaphysalis bispinosa<\/jats:italic>\n                      <\/jats:styled-content>\n                      . Statistical analysis indicated significantly higher infection rates in pet dogs and cats from the Nakhon Pathom region. Phylogenetic reconstruction with maximum\u2010likelihood and Bayesian\u2010inference approaches revealed distinct clustering of\n                      <jats:italic>R<\/jats:italic>\n                      .\n                      <jats:italic>asembonensis<\/jats:italic>\n                      and uncultured\n                      <jats:italic>Rickettsia<\/jats:italic>\n                      spp. Furthermore, three nucleotide sequence types of\n                      <jats:italic>R<\/jats:italic>\n                      .\n                      <jats:italic>asembonensis<\/jats:italic>\n                      identified here were unique and not shared with isolates from other countries or host species, as demonstrated by phylogenetic and Templeton\u2013Crandall\u2013Sing network analyses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This work provides the first documentation of\n                      <jats:italic>R<\/jats:italic>\n                      .\n                      <jats:italic>asembonensis<\/jats:italic>\n                      in client\u2010owned urban dogs and cats in Thailand and highlights the need for increased awareness among veterinarians and pet owners, given its public health relevance.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/zph.70048","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T19:04:33Z","timestamp":1772996673000},"page":"262-275","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["First Molecular Detection of the Potential Zoonotic Pathogen\n                    <i>Rickettsia asembonensis<\/i>\n                    in Client\u2010Owned Dogs and Cats in 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