{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T04:15:25Z","timestamp":1778040925655,"version":"3.51.4"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T00:00:00Z","timestamp":1774310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T00:00:00Z","timestamp":1774310400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"published-print":{"date-parts":[[2026,6]]},"abstract":"ABSTRACT<\/jats:title>\n \n Introduction<\/jats:title>\n \n Fleas represent a neglected group of ectoparasites of significant public health importance, due to their active role in transmitting various infectious agents associated with emerging and re\u2010emerging diseases. In Mexico, flea borne\u2010rickettsioses represent a growing public health concern, driven by the widespread presence of arthropod vectors and a vulnerable population, compounded by limited knowledge of pathogen\u2010vector\u2010host interactions. This study aimed to identify flea species parasitising dogs and cats in the state of Nuevo Leon, Mexico and to detect and characterise\n Rickettsia<\/jats:italic>\n species present through molecular sequencing.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Flea samples were collected from dogs and cats in veterinary clinics and private residences during the period of October\u2013May 2022\u20132023, across nine municipalities in the state of Nuevo Leon. Samples were identified morphologically and the\n glt<\/jats:italic>\n A gene for\n Rickettsia<\/jats:italic>\n was amplified and sequenced.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Three flea species were identified from dogs and cats:\n Ctenocephalides felis felis<\/jats:italic>\n (Bouch\u00e9, 1835),\n Pulex simulans<\/jats:italic>\n (Baker, 1895) and\n \n Echidnophaga gallinacea<\/jats:italic>\n <\/jats:styled-content>\n (Westwood, 1875). Rickettsia DNA was detected in 29.47% of the total flea pools through amplification of the citrate synthase (\n glt<\/jats:italic>\n A) gene. Sequence analysis revealed the presence of\n Candidatus<\/jats:italic>\n Rickettsia senegalensis in\n \n C. felis<\/jats:styled-content>\n felis\n <\/jats:italic>\n collected from dogs in Nuevo Le\u00f3n, representing the first record in Mexico. Simultaneously, an unexpected finding of\n \n Rickettsia rickettsii<\/jats:italic>\n <\/jats:styled-content>\n , the causative agent of Rocky Mountain spotted fever (RMSF), was also identified in the same flea species, marking the first report of this pathogen in fleas worldwide.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Our findings provide for the first time in Mexico, the molecular detection of\n Ca.<\/jats:italic>\n \n R. senegalensis<\/jats:italic>\n <\/jats:styled-content>\n in\n Ct. felis felis<\/jats:italic>\n , increases the number of\n Rickettsia<\/jats:italic>\n species recorded in the country to 19. Additionally,\n \n R. rickettsii<\/jats:italic>\n <\/jats:styled-content>\n DNA was detected in\n Ct. felis felis.<\/jats:italic>\n These findings highlight the need for further studies on clarify the epidemiological relevance of fleas\u2010associated\n Rickettsia<\/jats:italic>\n and to better understand their role within rickettsial systems.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/zph.70057","type":"journal-article","created":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T05:25:18Z","timestamp":1774416318000},"page":"370-379","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Flea Borne\u2010\n Rickettsia<\/i>\n Surveillance in Nuevo Leon, Mexico: An Unexpected Finding"],"prefix":"10.1111","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0067-6660","authenticated-orcid":false,"given":"Gisela A.","family":"Le\u00f3n\u2010Espinosa","sequence":"first","affiliation":[{"name":"Laboratorio de Entomolog\u00eda M\u00e9dica, Facultad de Ciencias Biol\u00f3gicas Universidad Aut\u00f3noma de Nuevo Le\u00f3n San Nicol\u00e1s de los Garza Nuevo Le\u00f3n M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana E.","family":"Flores\u2010Su\u00e1rez","sequence":"additional","affiliation":[{"name":"Laboratorio de Entomolog\u00eda M\u00e9dica, Facultad de Ciencias Biol\u00f3gicas Universidad Aut\u00f3noma de Nuevo Le\u00f3n San Nicol\u00e1s de los Garza Nuevo Le\u00f3n M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz","family":"Salceda\u2010S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Laboratorio de Entomolog\u00eda, Instituto de Diagn\u00f3stico y Referencia Epidemiol\u00f3gicos, Secretar\u00eda de Salud Ciudad de M\u00e9xico M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6316-2187","authenticated-orcid":false,"given":"Sokani","family":"S\u00e1nchez\u2010Montes","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Biol\u00f3gicas y Agropecuarias, Regi\u00f3n Poza Rica\u2010Tuxpan Universidad Veracruzana Tuxpan de Rodr\u00edguez Cano Veracruz M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jes\u00fas A.","family":"D\u00e1vila\u2010Barboza","sequence":"additional","affiliation":[{"name":"Laboratorio de Entomolog\u00eda M\u00e9dica, Facultad de Ciencias Biol\u00f3gicas Universidad Aut\u00f3noma de Nuevo Le\u00f3n San Nicol\u00e1s de los Garza Nuevo Le\u00f3n M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz","family":"L\u00f3pez\u2010Monroy","sequence":"additional","affiliation":[{"name":"Laboratorio de Entomolog\u00eda M\u00e9dica, Facultad de Ciencias Biol\u00f3gicas 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