{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T07:08:47Z","timestamp":1788851327956,"version":"build-2803163510"},"reference-count":51,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100006692","name":"Universit\u00e0 degli Studi di Torino","doi-asserted-by":"publisher","award":["Ricerca Locale 2023\u2010Linea B\u2010RUBS_RILO_23_01"],"award-info":[{"award-number":["Ricerca Locale 2023\u2010Linea B\u2010RUBS_RILO_23_01"]}],"id":[{"id":"10.13039\/501100006692","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Zoonoses and Public Health"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Wild boars and domestic pigs can act as intermediate hosts for two\n                      <jats:italic>Sarcocystis<\/jats:italic>\n                      species:\n                      <jats:italic>Sarcocystis miescheriana<\/jats:italic>\n                      and the zoonotic\n                      <jats:italic>Sarcocystis suihominis<\/jats:italic>\n                      . Humans become infected with\n                      <jats:italic>S. suihominis<\/jats:italic>\n                      by eating raw or undercooked meat. Herein we investigated the occurrence of\n                      <jats:italic>Sarcocystis<\/jats:italic>\n                      spp. in wild boars hunted for human consumption in Poland.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Diaphragms were collected from 241 wild boars during two hunting seasons; a multiplex\u2010PCR protocol targeting the mtDNA\n                      <jats:italic>cox1<\/jats:italic>\n                      gene of\n                      <jats:italic>S. miescheriana<\/jats:italic>\n                      and\n                      <jats:italic>S. suihominis<\/jats:italic>\n                      was applied; diaphragm samples testing positive for\n                      <jats:italic>S. suihominis<\/jats:italic>\n                      underwent sarcocysts isolation and molecular characterization.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Out of 241 wild boars, 216 (90%) revealed the presence of\n                      <jats:italic>S. miescheriana<\/jats:italic>\n                      DNA, including eight (3.3%) co\u2010infected with\n                      <jats:italic>S. suihominis<\/jats:italic>\n                      . The diaphragms of three wild boars testing positive for\n                      <jats:italic>S. suihominis<\/jats:italic>\n                      and\n                      <jats:italic>S. miescheriana<\/jats:italic>\n                      were further processed, yielding 37 sarcocysts. Two out of 37 isolated sarcocysts tested positive for\n                      <jats:italic>S. suihominis<\/jats:italic>\n                      and were further molecularly characterized, showing 98.9%\u2013100% identity on the 18S rRNA gene and 98.7% identity on the\n                      <jats:italic>cox1<\/jats:italic>\n                      mtDNA gene with\n                      <jats:italic>S. suihominis<\/jats:italic>\n                      . Thirteen of the remaining sarcocysts were molecularly characterized, leading to the identification of\n                      <jats:italic>S. miescheriana<\/jats:italic>\n                      in 12 cysts; one out of the 13 sarcocysts yielded a 1053\u2009bp\n                      <jats:italic>cox1<\/jats:italic>\n                      mtDNA sequence and a 923\u2009bp 18S rDNA sequence showing &lt;\u200989.1% and 95.6% homology, respectively, with any known\n                      <jats:italic>Sarcocystis<\/jats:italic>\n                      species.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This is the first study identifying\n                      <jats:italic>Sarcocystis<\/jats:italic>\n                      spp. circulating in wild boars intended for human consumption in Poland, revealing a non\u2010negligible rate of infection with the zoonotic\n                      <jats:italic>S. suihominis,<\/jats:italic>\n                      a high rate of infection with\n                      <jats:italic>S. miescheriana<\/jats:italic>\n                      and the presence of a putative new\n                      <jats:italic>Sarcocystis<\/jats:italic>\n                      sp. Our study highlights that the risk for the consumers of undercooked wild boar meat cannot be excluded.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/zph.70092","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T06:36:59Z","timestamp":1788849419000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Detection of\n                    <i>Sarcocystis suihominis<\/i>\n                    and\n                    <i>Sarcocystis miescheriana<\/i>\n                    in Wild Boars Hunted for Human Consumption in Poland Highlights a Potential Risk for the Consumer"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8266-581X","authenticated-orcid":false,"given":"Weronika","family":"Korpysa\u2010Dzirba","sequence":"first","affiliation":[{"name":"Department of Parasitology and Invasive Diseases National Veterinary Research 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