{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T05:10:23Z","timestamp":1775365823021,"version":"3.50.1"},"reference-count":111,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T00:00:00Z","timestamp":1759190400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T00:00:00Z","timestamp":1759190400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["ift.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Food Science"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Avocados are a valuable source of lipids with potential applications in food, nutraceutical, and cosmetic products, all requiring food\u2010grade and environmentally friendly extraction methods. Though often enjoyed fresh, avocado pulp is also processed for oil. However, conventional extraction techniques can compromise pulp lipid composition, triggering the need for alternative methods that preserve pulp lipid integrity and meet industrial sustainability standards. Ultrasound probe\u2010assisted extraction using ethanol, a green solvent, could be a sustainable alternative to extract lipids from avocado pulp. In the present study, lipid profiles from\n                      <jats:italic>Hass<\/jats:italic>\n                      Portuguese avocado pulp were characterized using C18 reversed\u2010phase HPLC\u2010MS\/MS, and their biological activities were evaluated. Folch\u2010like extraction was employed as a benchmark for comparison. Untargeted lipidomics profiling revealed over 100 lipid species across polar and neutral classes, with triacylglycerides enriched in essential polyunsaturated fatty acids. Ultrasound\u2010assisted ethanol extraction achieved \u224870% lipid purity and yielded a lipid composition comparable to that of the conventional method. Notably, green pulp extract exhibited significant bioactivity, including moderate antioxidant activity and powerful anti\u2010inflammatory effects, inhibiting COX\u20102 and reducing nitric oxide levels at low concentrations. These findings support the use of ultrasound\u2010assisted ethanol extraction as an efficient and sustainable strategy for obtaining bioactive compounds from avocado pulp, reinforcing its potential incorporation into high\u2010value or even new formulations.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Practical Applications<\/jats:title>\n                    <jats:p>\n                      This study shows an eco\u2010friendly method to extract healthy lipids from\n                      <jats:italic>Hass<\/jats:italic>\n                      avocado pulp using ultrasound\u2010assisted ethanol extraction technique. This method is greener than traditional ones and produces extracts with antioxidant and anti\u2010inflammatory benefits. These natural extracts can be used food, cosmetic, and nutraceutical or supplement industries as natural ingredients applications.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/1750-3841.70550","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T10:30:27Z","timestamp":1759228227000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Lipidomics\u2010Guided Ultrasound\u2010Assisted Ethanol Extraction of Avocado Pulp and Evaluation of Bioactive Properties"],"prefix":"10.1111","volume":"90","author":[{"given":"Bruna B.","family":"Neves","sequence":"first","affiliation":[{"name":"Mass Spectrometry Centre, LAQV\u2010REQUIMTE, Department of Chemistry University of Aveiro, Santiago University Campus  Aveiro Portugal"},{"name":"CESAM\u2014Centre for Environmental and Marine Studies, Department of Chemistry University of Aveiro, Santiago University Campus  Aveiro Portugal"}]},{"given":"Rita","family":"Pais","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Centre, LAQV\u2010REQUIMTE, Department of Chemistry University of Aveiro, Santiago University Campus  Aveiro Portugal"},{"name":"CESAM\u2014Centre for Environmental and Marine Studies, Department of Chemistry University of Aveiro, Santiago University Campus  Aveiro Portugal"}]},{"given":"Joana","family":"Batista","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Centre, LAQV\u2010REQUIMTE, Department of Chemistry University of Aveiro, Santiago University Campus  Aveiro Portugal"},{"name":"CESAM\u2014Centre for Environmental and Marine Studies, Department of Chemistry University of Aveiro, Santiago University Campus  Aveiro Portugal"}]},{"given":"Marisa","family":"Pinho","sequence":"additional","affiliation":[{"name":"CESAM\u2014Centre for Environmental and Marine Studies, Department of Chemistry University of Aveiro, Santiago University Campus  Aveiro Portugal"}]},{"given":"Tatiana","family":"Maur\u00edcio","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Centre, LAQV\u2010REQUIMTE, Department of Chemistry University of Aveiro, Santiago University Campus  Aveiro Portugal"},{"name":"CESAM\u2014Centre for Environmental and Marine Studies, Department of Chemistry University of Aveiro, Santiago University Campus  Aveiro Portugal"},{"name":"Department of Medical Sciences, Institute of Biomedicine (iBiMED) University of Aveiro, Santiago University Campus  Aveiro Portugal"}]},{"given":"Stefano","family":"Bonciarelli","sequence":"additional","affiliation":[{"name":"Molecular Discovery Ltd., Centennial Park, Borehamwood  Hertfordshire UK"}]},{"given":"Laura","family":"Goracci","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Biology and Biotechnology University of Perugia  Perugia Italy"}]},{"given":"Bruno","family":"Neves","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences, Institute of Biomedicine (iBiMED) University of Aveiro, Santiago University Campus  Aveiro Portugal"}]},{"given":"Pedro","family":"Domingues","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Centre, LAQV\u2010REQUIMTE, Department of Chemistry University of Aveiro, Santiago University Campus  Aveiro Portugal"}]},{"given":"M. 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