{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T05:55:01Z","timestamp":1779342901300,"version":"3.51.4"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,11,14]],"date-time":"2013-11-14T00:00:00Z","timestamp":1384387200000},"content-version":"vor","delay-in-days":5706,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[1998,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fimbriae preparation from <jats:italic>Actinobacillus actinomycetemcomitans<\/jats:italic> was found to contain an abundant low\u2010molecular\u2010weight protein (termed Flp) with an apparent molecular mass of approximately 6.5 kDa, in addition to a small amount of 54\u2010kDa protein. Immunogold electron microscopy localized the Flp protein at the bacterial fimbriae but not at the cell surface. The DNA fragment including the <jats:italic>flp<\/jats:italic> gene was cloned from A. <jats:italic>actinomycetemcomitans<\/jats:italic> 304\u2010a and its nucleotide sequence was determined. An open reading frame of the <jats:italic>flp<\/jats:italic> gene was composed of 225 bp encoding a protein of 75 amino acids. Comparison of the translated amino acid sequence with the sequence of native Flp determined by Edman degradation indicated that the N\u2010terminal part of 26 amino acids is leader peptide. The N\u2010terminal sequence of mature Flp exhibited some similarity to type\u2010IV pilin. Furthermore, the processing site of premature Flp is also similar to that of type\u2010IV prepilin, and a gene encoding a protein homologous to type\u2010IV prepilin\u2010like protein leader peptidase was found downstream of the <jats:italic>flp<\/jats:italic> gene. These findings indicate that Flp is the major component protein of <jats:italic>A. actinomycetemcomitans<\/jats:italic> fimbriae.<\/jats:p>","DOI":"10.1111\/j.1348-0421.1998.tb02280.x","type":"journal-article","created":{"date-parts":[[2013,11,14]],"date-time":"2013-11-14T16:28:30Z","timestamp":1384446510000},"page":"253-258","source":"Crossref","is-referenced-by-count":77,"title":["Molecular Characterization of Low\u2010Molecular\u2010Weight Component Protein, Flp, in <i>Actinobacillus actinomycetemcomitans<\/i> Fimbriae"],"prefix":"10.1111","volume":"42","author":[{"given":"Tetsuyoshi","family":"Inoue","sequence":"first","affiliation":[{"name":"Department of Microbiology Okayama University Dental School  Okayama Okayama 700\u20108525 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ichiro","family":"Tanimoto","sequence":"additional","affiliation":[{"name":"Department of Microbiology Okayama University Dental School  Okayama Okayama 700\u20108525 Japan"},{"name":"Periodontology and Endodontology Okayama University Dental School  Okayama Okayama 700\u20108525 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Ohta","sequence":"additional","affiliation":[{"name":"Department of Microbiology Okayama University Dental School  Okayama Okayama 700\u20108525 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keijiro","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Microbiology Okayama University Dental School  Okayama Okayama 700\u20108525 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoji","family":"Murayama","sequence":"additional","affiliation":[{"name":"Periodontology and Endodontology Okayama University Dental School  Okayama Okayama 700\u20108525 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiro","family":"Fukui","sequence":"additional","affiliation":[{"name":"Department of Microbiology Okayama University Dental School  Okayama Okayama 700\u20108525 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,11,14]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90508-9"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01949.x"},{"key":"e_1_2_2_5_1","first-page":"2730","volume-title":"Escherichia coli and Salmonella typhimurium","author":"Hultgren S.J.","year":"1996"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1990.tb04167.x"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1997.0137"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1139\/m93-071"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(83)90040-9"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1987.tb03092.x"},{"key":"e_1_2_2_12_1","first-page":"3485","article-title":"Effect of bicarbonate on the growth of Actinobacillus actinomycetemcomitans in anaerobic fructose\u2010limited chemostat culture","volume":"135","author":"Ohta H.","year":"1989","journal-title":"J. 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