{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T04:53:56Z","timestamp":1761540836912},"reference-count":13,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2008,3,12]],"date-time":"2008-03-12T00:00:00Z","timestamp":1205280000000},"content-version":"vor","delay-in-days":12065,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[1975,3]]},"abstract":"<jats:p><jats:bold>S<jats:sc>ummary<\/jats:sc>. <\/jats:bold> Separation of human platelets from plasma by a modified gel\u2010filtration technique reveals very low levels of factor\u2010V activity of the platelet suspension. Repeated freezing and thawing increases the factor\u2010V activity in various factor\u2010V assays. This activity neutralized the inactivating effect of a rabbit\u2010antihuman factor V antibody to plasma factor V, while intact platelets had almost no such capacity. Washed and normal platelets and gel filtered platelets showed marked positive fluorescence after treatment with antifactor V serum and FITC labelled sheep anti\u2010rabbit immunoglobulin. Fluorescence was inhibited by previous incubation of the antifactor V serum and platelet lysates. Platelets of a factor V deficient patient showed the same fluorescence pattern as normal platelets indicating that they contained a factor V antigen. These platelets showed after lysis no effect in various factor V assays. From these studies it is concluded that the localization of factor V is within the platelets.<\/jats:p>","DOI":"10.1111\/j.1365-2141.1975.tb01838.x","type":"journal-article","created":{"date-parts":[[2008,3,12]],"date-time":"2008-03-12T17:57:13Z","timestamp":1205344633000},"page":"405-412","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["The Localization of Factor V within Normal Human Platelets and the Demonstration of a Platelet\u2010Factor V Antigen in Congenital Factor V Deficiency"],"prefix":"10.1111","volume":"29","author":[{"given":"K.","family":"Breederveld","sequence":"first","affiliation":[]},{"given":"J. C.","family":"Giddings","sequence":"additional","affiliation":[]},{"given":"J. W.","family":"ten Cate","sequence":"additional","affiliation":[]},{"given":"A. 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