{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T07:18:30Z","timestamp":1778570310183,"version":"3.51.4"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,10,27]],"date-time":"2006-10-27T00:00:00Z","timestamp":1161907200000},"content-version":"vor","delay-in-days":6084,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[1990,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>P.11 outer membrane proteins of <jats:italic>Neisseria gonorrhoeae<\/jats:italic> are encoded by a family of closely related genes. Although the genes are highly conserved, major differences in sequence among them occur in two short regions, designated hypervariable regions 1 (HV<jats:sub>1<\/jats:sub>) and 2 (HV<jats:sub>2<\/jats:sub>). In this study, we determined the number and DNA sequence of the hypervariable regions in the P.11 genes of strain FA1090. The FA1090 chromosome contained at least eleven P.11 loci, having six different versions each of HV<jats:sub>1<\/jats:sub> and HV<jats:sub>2<\/jats:sub> among them. Southern blotting with HV\u2010specific oligonucleotides showed that each version was present in one to three copies, and that there were nine unique combinations of HV<jats:sub>1<\/jats:sub> and HV<jats:sub>2<\/jats:sub> in the P.M genes. Although each of the versions of HV<jats:sub>1<\/jats:sub> or HV<jats:sub>2<\/jats:sub> had a unique DNA sequence, there were some similarities among them, particularly when certain ones were compared. Restriction fragments containing only the HV regions were cloned into an expression vector to demonstrate that the epitopes recognized by a set of monoclonal antibodies specific for different FA1090 P.11 proteins were completely encoded by either HV<jats:sub>1<\/jats:sub> or HV<jats:sub>2<\/jats:sub>.<\/jats:p>","DOI":"10.1111\/j.1365-2958.1990.tb00610.x","type":"journal-article","created":{"date-parts":[[2006,10,27]],"date-time":"2006-10-27T21:27:20Z","timestamp":1161984440000},"page":"439-449","source":"Crossref","is-referenced-by-count":50,"title":["Characterization of the repertoire of hypervariable regions in the Protein II <i>(opa)<\/i> gene family of <i>Neisseria gonorrhoeae<\/i>"],"prefix":"10.1111","volume":"4","author":[{"given":"T. D.","family":"Connelly","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Shaffer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. G.","family":"Cannon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,10,27]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"2026","DOI":"10.1128\/iai.55.9.2026-2031.1987","article-title":"Antigenic and structural differences among proteins 11 expressed by a single strain of Neisseria gonorrhoeae","volume":"55","author":"Barritt D.S.","year":"1987","journal-title":"Infect Immun"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1989.tb00226.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1128\/iai.54.1.154-160.1986","article-title":"Interactions of gonococci with HeLa cells: attachment, detachment, replication, penetration, and the role of protein H","volume":"54","author":"Bessen D.","year":"1986","journal-title":"Infect Immun"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1128\/iai.45.2.453-457.1984","article-title":"Characterization of Neisseria gonorrhoeae protein 11 phase variation by use of monoclonal antibodies","volume":"45","author":"Black W.J.","year":"1984","journal-title":"Infect Immun"},{"key":"e_1_2_1_6_1","first-page":"377","volume-title":"Bacterial Outer Membranes as Model Systems","author":"Blake M.S.","year":"1987"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1988.tb00024.x"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/12.1Part1.387"},{"key":"e_1_2_1_9_1","first-page":"585","article-title":"Antigenic variation of outer membrane proteins 11 in colonial variants of Neisseria gonorrhoeae P9","volume":"128","author":"Diaz J.L.","year":"1982","journal-title":"J Clin Microbiol"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1128\/iai.27.3.882-888.1980","article-title":"Comparison of virulence markers of peritoneal and fallopian tube isolates with endocervical Neisseria gonorrhoeae isolates from women with acute salpingitis","volume":"27","author":"Draper D.L.","year":"1980","journal-title":"Infect tmmun"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"1574","DOI":"10.1128\/iai.56.6.1574-1579.1988","article-title":"Gonococci possessing only certain PII outer membrane proteins interact with human neutrophils","volume":"56","author":"Fischer S.H.","year":"1988","journal-title":"Infect Immun"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/338651a0"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1128\/jb.145.2.736-742.1981","article-title":"Structural comparison of Neisseria gonorrhoeae outer membrane proteins","volume":"145","author":"Heckels J.E.","year":"1981","journal-title":"J Bacteriol"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.6.3824"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1128\/iai.19.1.332-340.1978","article-title":"Studies on gonococcus infection. 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Cell wall protein differences among color\/opacity colony variants of Neisseria gonorrhoeae","volume":"21","author":"Swanson J.","year":"1978","journal-title":"Infect Immun"},{"key":"e_1_2_1_35_1","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1128\/iai.28.1.54-64.1980","article-title":"125I\u2010labeled peptide mapping of some heat\u2010modifiable proteins of the gonococcal outer membrane","volume":"281","author":"Swanson J.","year":"1980","journal-title":"Infect Immun"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.37.1.359-368.1982"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.157.5.1405"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.168.6.2121"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1988.tb00091.x"},{"key":"e_1_2_1_40_1","first-page":"503","article-title":"The effect of Protein II and pili on the interaction of Neisseria gonorrhoeae with human polymor\u2010phonuclear leucocytes","volume":"132","author":"Virji M.","year":"1986","journal-title":"J Gen Microbiol"},{"key":"e_1_2_1_41_1","first-page":"843","article-title":"Double\u2010stranded DNA sequencing with T7 polymerase","volume":"6","author":"Wang Y.","year":"1988","journal-title":"Bio Techniques"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1989.tb00205.x"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.5.1194"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/149.2.166"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2958.1990.tb00610.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2958.1990.tb00610.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T14:54:30Z","timestamp":1697986470000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2958.1990.tb00610.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,3]]},"references-count":43,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1990,3]]}},"alternative-id":["10.1111\/j.1365-2958.1990.tb00610.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2958.1990.tb00610.x","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,3]]}}}