{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T15:09:59Z","timestamp":1773673799313,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2008,12,11]],"date-time":"2008-12-11T00:00:00Z","timestamp":1228953600000},"content-version":"vor","delay-in-days":4119,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tissue Antigens"],"published-print":{"date-parts":[[1997,9]]},"abstract":"<jats:p>Endoglin (CD 105) is a homodimeric cell surface component of the TGF\u00df1 receptor complex, which is expressed at high levels on vascular endothelium and at lower levels on activated monocytes. It is also the target gene for the dominantly inherited vascular disorder hereditary hemorrhagic telangiectasia type 1. To date, each family has a distinct endoglin mutation, most of which generate premature stop codons. The purpose of the current study was to identify monoclonal antibodies capable of binding to normal and mutated forms of the protein. We generated stable transfectants of fulllength human endoglin in murine fibroblasts and engineered and expressed in bacteria several fragments of the extracellular domain. Relatively pure polypeptides were recovered with good yield from inclusion bodies and were tested by ELISA and Western blot; 11 monoclonal antibodies were shown to react specifically with the endoglin transfectants. Ten of these monoclonal antibodies reacted with the bacterial fragments, and their epitopes were assigned to 3 distinct regions of endoglin. Monoclonal antibodies P3D1, TEC4 and GRE reacted with the <jats:italic>N<\/jats:italic>\u2010terminal region of 204 amino acids encoded by exons 1 to 5. Monoclonal antibodies P4A4, 44G4, E\u20109, MAEND3 and PN\u2010E2 all bound to a region of 54 amino acids encoded mostly by exon 7. Monoclonal antibodies CLE4 and RMAC8 reacted with the <jats:italic>C<\/jats:italic>\u2010terminal region of the extracellular domain, coded for by exons 8 to 12. Knowing the localization of these epitopes will facilitate the structural and functional analysis of normal and mutated forms of endoglin.<\/jats:p>","DOI":"10.1111\/j.1399-0039.1997.tb02870.x","type":"journal-article","created":{"date-parts":[[2008,12,11]],"date-time":"2008-12-11T15:09:03Z","timestamp":1229008143000},"page":"265-276","source":"Crossref","is-referenced-by-count":52,"title":["Mapping epitopes to distinct regions of the extracellular domain of endoglin using bacterially expressed recombinant fragments"],"prefix":"10.1111","volume":"50","author":[{"given":"S.","family":"Pichuantes","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Vera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Bourdeau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Pece","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.A.","family":"Wayner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Letarte","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,12,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.4049\/jimmunol.134.2.1276","article-title":"Identification of several cell surface proteins of non\u2010T, non\u2010B acute lymphoblastic leukemia by using monoclonal antibodies","volume":"134","author":"Quackenbush EJ","year":"1985","journal-title":"J Immunol"},{"key":"e_1_2_1_3_2","first-page":"841","article-title":"Endoglin is expressed on a subpopulation of immature erythroid cells of normal human bone marrow","volume":"5","author":"Buhring H\u2010J","year":"1991","journal-title":"Leukemia"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"4456","DOI":"10.4049\/jimmunol.154.9.4456","article-title":"Differential expression of endoglin in fetal and adult hematopoietic cells in human bone marrow","volume":"154","author":"Rokhlin OW","year":"1995","journal-title":"J Immunol"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1996.d01-1698.x"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"565","DOI":"10.4049\/jimmunol.156.2.564","article-title":"Endoglin is a component of the TGF\u2010\u00df receptor complex of human pre\u2010B leukemic cells","volume":"156","author":"Zhang HW","year":"1996","journal-title":"J Immunol"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"1995","DOI":"10.1016\/S0021-9258(17)42126-0","article-title":"Endoglin forms a heteromeric complex with the signaling receptors for transforming growth factor\u2010\u00df","volume":"269","author":"Yamashita H","year":"1994","journal-title":"J Biol Chem"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830220216"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1992.tb05848.x"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.133.5.1109"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"19027","DOI":"10.1016\/S0021-9258(18)41732-2","article-title":"Endoglin is a component of the transforming growth factor\u2010\u00df receptor system in human endothelial cells","volume":"267","author":"Cheifetz S","year":"1992","journal-title":"J Biol Cliem"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"4206","DOI":"10.4049\/jimmunol.153.9.4206","article-title":"Functional regulation of platelet\/endothelial cell adhesion molecule\u20101 by TGF\u2010\u00df1 in promonocytic U\u2010937 cells","volume":"153","author":"Lastres P","year":"1994","journal-title":"J Immunol"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.141.6.1925"},{"key":"e_1_2_1_14_2","first-page":"1756","volume-title":"Leukocyte typing. 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