{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:32:41Z","timestamp":1759336361828},"reference-count":15,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,12,11]],"date-time":"2008-12-11T00:00:00Z","timestamp":1228953600000},"content-version":"vor","delay-in-days":4089,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tissue Antigens"],"published-print":{"date-parts":[[1997,10]]},"abstract":"<jats:p>Unrelated volunteer donors (69) recruited by the National Marrow Donor Program were HLA typed by DNA\u2010based methods for both the HLA\u2010A and \u2010B loci. Each donor had been previously typed by serology by at least two independent laboratories. Of the 69 samples, all serologic laboratories were in concordance for HLA\u2010A in 62 typed samples and for HLA\u2010B in 48 typed samples. Of the serologically concordant samples, 5 samples typed for HLA\u2010A and 7 samples typed for HLA\u2010B received DNA and serology types differing in their level of resolution. One sample typed for HLA\u2010A and 3 samples typed for HLA\u2010B by DNA methods gave different results from their serologic assignments. Of the samples exhibiting disparities among the different serologic typing laboratories, the DNA\u2010defined types of 7 samples typed for HLA\u2010A and 18 samples typed for HLA\u2010B were consistent with at least one of the serologic assignments. The DNA types for the remaining 3 HLA\u2010B typed samples did not agree with the serologic assignments and their alleles were subsequently sequenced. One of these sequences was a previously undefined allele, B*1537. Sharing of polymorphic sequences among HLA allelic products creates difficulties for consistent serologic assignments of some types complicating the process of identifying potential donors from bone marrow registries. Thus, the use of DNA\u2010based typing techniques for characterization of donor class I types should allow a more consistent definition of types and should speed the donor selection process.<\/jats:p>","DOI":"10.1111\/j.1399-0039.1997.tb02889.x","type":"journal-article","created":{"date-parts":[[2008,12,11]],"date-time":"2008-12-11T15:05:39Z","timestamp":1229007939000},"page":"366-371","source":"Crossref","is-referenced-by-count":15,"title":["Analysis of HLA\u2010A and \u2010B serologic typing of bone marrow registry donors using polymerasee chain reaction with sequence\u2010specific oligonucleotide probes and DNA sequencing"],"prefix":"10.1111","volume":"50","author":[{"given":"D.M.","family":"Sintasath","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Bel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Steiner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Ng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Alosco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.D.","family":"Hegland","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.K.","family":"Hurley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,12,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5258.67"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0198-8859(94)90270-4"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1995.tb02470.x"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1992.tb01912.x"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4612-3552-1_2"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1993.tb01992.x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1995.tb02408.x"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1996.tb02594.x"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1995.tb02482.x"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0198-8859(94)90083-3"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1996.tb02538.x"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/329506a0"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1995.tb03143.x"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0198-8859(92)90025-I"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1995.tb03124.x"}],"container-title":["Tissue Antigens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1399-0039.1997.tb02889.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1399-0039.1997.tb02889.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T09:54:56Z","timestamp":1698400496000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1399-0039.1997.tb02889.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,10]]},"references-count":15,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1997,10]]}},"alternative-id":["10.1111\/j.1399-0039.1997.tb02889.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1399-0039.1997.tb02889.x","archive":["Portico"],"relation":{},"ISSN":["0001-2815","1399-0039"],"issn-type":[{"value":"0001-2815","type":"print"},{"value":"1399-0039","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,10]]}}}