{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,8]],"date-time":"2026-02-08T05:25:01Z","timestamp":1770528301186,"version":"3.49.0"},"reference-count":6,"publisher":"Wiley","issue":"1-4","license":[{"start":{"date-parts":[[2009,1,9]],"date-time":"2009-01-09T00:00:00Z","timestamp":1231459200000},"content-version":"vor","delay-in-days":14284,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Protein Research"],"published-print":{"date-parts":[[1969,12]]},"abstract":"<jats:p> <jats:italic>The incorporation of amino acids into TCA <\/jats:italic><jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"#fn1\"><jats:italic>*<\/jats:italic><\/jats:ext-link><jats:italic> precipitable polypeptides has been studied in a cell\u2010free system isolated from rat liver. A new procedure is described for the isolation of all amino\u2010acyl\u2010tRNA synthetases necessary for optimal amino acid incorporation. Polyribosomes were present either as membrane\u2010bound or as free polyribosomes. Differences in incorporation between bound and free polyribosomes were observed. In contrast to free polyribosomes the incorporation of (<jats:sup>14<\/jats:sup>C)leucine in bound polyribosomes was not stimulated by addition of all the other non\u2010radioactive amino acids. The plateau for incorporation of (<jats:sup>14<\/jats:sup>C)leucine into bound polyribosomes was reached after 5 min and for free polyribosomes after 60 min. From these findings it was concluded that<\/jats:italic> in vitro, <jats:italic>apart from a limited translation, further translation of bound polyribosomes was blocked.<\/jats:italic><\/jats:p>","DOI":"10.1111\/j.1399-3011.1969.tb01643.x","type":"journal-article","created":{"date-parts":[[2010,8,18]],"date-time":"2010-08-18T08:25:55Z","timestamp":1282119955000},"page":"193-197","source":"Crossref","is-referenced-by-count":9,"title":["THE INCORPORATION OF AMINO ACIDS BY FREE POLYRIBOSOMES AND ROUGH ENDOPLASMIC RETICULUM IN A CELL\u2010FREE SYSTEM"],"prefix":"10.1111","volume":"1","author":[{"given":"W. S.","family":"Bont","sequence":"first","affiliation":[]},{"given":"J.","family":"Geels","sequence":"additional","affiliation":[]},{"given":"G.","family":"Rezelman","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2009,1,9]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"177","volume":"87","author":"Bloemendal H.","year":"1964","journal-title":"Biochim. biophys. Acta"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1030177"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(67)90425-0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)97223-6"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)65898-3"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2787(67)90674-0"}],"container-title":["International Journal of Protein Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1399-3011.1969.tb01643.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1399-3011.1969.tb01643.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,30]],"date-time":"2023-08-30T10:35:09Z","timestamp":1693391709000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1399-3011.1969.tb01643.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1969,12]]},"references-count":6,"journal-issue":{"issue":"1-4","published-print":{"date-parts":[[1969,12]]}},"alternative-id":["10.1111\/j.1399-3011.1969.tb01643.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1399-3011.1969.tb01643.x","archive":["Portico"],"relation":{},"ISSN":["0020-7551"],"issn-type":[{"value":"0020-7551","type":"print"}],"subject":[],"published":{"date-parts":[[1969,12]]}}}