{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T04:10:26Z","timestamp":1700712626028},"reference-count":12,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,3,3]],"date-time":"2005-03-03T00:00:00Z","timestamp":1109808000000},"content-version":"vor","delay-in-days":12633,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[1970,8]]},"abstract":"<jats:p>The kinetics of the puromycin interaction with different ribosomal bound peptidyl\u2010tRNAs were studied. The rates of the puromycin reaction with AA\u2010Phe\u2010tRNA and AA<jats:sub>2<\/jats:sub>\u2010Phe\u2010tRNA using three different amino acids (AA) were compared. At 4\u00b0 all the three dipeptidyl\u2010tRNAs<jats:sup>Phe<\/jats:sup> reacted considerably slower than the three tripeptidyl\u2010tRNAs<jats:sup>Phe<\/jats:sup>. Acetyl\u2010Phe\u2010tRNA reacted faster than the dipeptidyl\u2010tRNA, but slower than the tripeptidyl\u2010tRNA. Gly<jats:sub>n<\/jats:sub>\u2010Phe\u2010tRNA (<jats:italic>n<\/jats:italic>= 3, 6 and 9) reacted with puromyein much faster than Gly\u2010Phe\u2010tRNA and at a rate similar to that of Gly<jats:sub>2<\/jats:sub>\u2010Phe\u2010tRNA.<\/jats:p><jats:p>Lauroyl\u2010Phe\u2010tRNA reacted with puromyein much slower than acetyl\u2010and octanoyl\u2010Phe\u2010tRNA both at 4\u00b0 and 30\u00b0<\/jats:p>","DOI":"10.1111\/j.1432-1033.1970.tb00998.x","type":"journal-article","created":{"date-parts":[[2005,3,3]],"date-time":"2005-03-03T12:41:31Z","timestamp":1109853691000},"page":"222-225","source":"Crossref","is-referenced-by-count":13,"title":["Substrate Specificity of <i>Escherichia coli<\/i> Peptidyl\u2010Transferase"],"prefix":"10.1111","volume":"15","author":[{"given":"Amos","family":"Panet","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan","family":"de Groot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yehuda","family":"Lapidot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,3,3]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(68)80028-2"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1968.tb00450.x"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1969.tb00571.x"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.61.3.1042"},{"key":"e_1_2_1_6_2","first-page":"375","volume-title":"Procedures in Nucleic Acid Research","author":"Muench K. H.","year":"1966"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1969.tb00565.x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2787(69)90081-1"},{"key":"e_1_2_1_9_2","unstructured":"Lapidot Y. Elat D. Rappoport S. anddeGroot N. Biochem. Biophys. Res. Comm. in press."},{"key":"e_1_2_1_10_2","unstructured":"Hamburger A. D. deGroot N. andLapidot Y. Biochim. Biophys. Acta in press."},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.57.4.1050"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(66)90586-9"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.145.3639.1399"}],"container-title":["European Journal of Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1432-1033.1970.tb00998.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1432-1033.1970.tb00998.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T03:42:56Z","timestamp":1700710976000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1111\/j.1432-1033.1970.tb00998.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1970,8]]},"references-count":12,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1970,8]]}},"alternative-id":["10.1111\/j.1432-1033.1970.tb00998.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1432-1033.1970.tb00998.x","archive":["Portico"],"relation":{},"ISSN":["0014-2956","1432-1033"],"issn-type":[{"value":"0014-2956","type":"print"},{"value":"1432-1033","type":"electronic"}],"subject":[],"published":{"date-parts":[[1970,8]]}}}