{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:50:33Z","timestamp":1761058233822},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,3,3]],"date-time":"2005-03-03T00:00:00Z","timestamp":1109808000000},"content-version":"vor","delay-in-days":11994,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[1972,5]]},"abstract":"<jats:p>\u03b1\u2010Lactalbumin was isolated from human milk in a yield of 1.8 mg\/ml of milk. The purification procedure involved ammonium sulphate fractionation (30% to 80% saturation) and pH\u20104.0 precipitation, followed by gel filtration with Sephadex G\u2010100. A final purification stage using DEAE\u2010cellulose was necessary in some preparations.<\/jats:p><jats:p>Peptides derived from the reduced, <jats:italic>S<\/jats:italic>\u2010aminoethylated protein by treatment with cyanogen bromide and digestion of these CNBr fragments with trypsin, chymotrypsin or thermolysin, were purified by gel filtration, ion exchange chromatography and high\u2010voltage paper electrophoresis.<\/jats:p><jats:p>From the sequences of these peptides it has proved possible to deduce unambiguously the complete primary structure of the protein. Comparison with bovine \u03b1\u2010lactalbumin shows an identity in 72% of the residues with a further 6% being chemically similar amino acids. The corresponding figures for the human \u03b1\u2010lactalbumin\/human lysozyme comparison, are 39% and 12%, respectively. The significance of some of the amino acid replacements is discussed.<\/jats:p>","DOI":"10.1111\/j.1432-1033.1972.tb01812.x","type":"journal-article","created":{"date-parts":[[2005,3,3]],"date-time":"2005-03-03T14:46:49Z","timestamp":1109861209000},"page":"65-86","source":"Crossref","is-referenced-by-count":124,"title":["The Complete Amino\u2010Acid Sequence of Human \u03b1\u2010Lactalbumin"],"prefix":"10.1111","volume":"27","author":[{"given":"John B. C.","family":"Findlay","sequence":"first","affiliation":[]},{"given":"Keith","family":"Brew","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,3,3]]},"reference":[{"key":"e_1_2_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)95873-4"},{"key":"e_1_2_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)96903-6"},{"key":"e_1_2_3_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.59.2.491"},{"key":"e_1_2_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/222671a0"},{"key":"e_1_2_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/newbio232016a0"},{"key":"e_1_2_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)63827-5"},{"key":"e_1_2_3_8_2","doi-asserted-by":"crossref","first-page":"1997","DOI":"10.1016\/S0021-9258(18)97416-8","volume":"240","author":"Canfield R. E.","year":"1965","journal-title":"J. Biol. Chem."},{"key":"e_1_2_3_9_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1967.0033"},{"key":"e_1_2_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)63826-3"},{"key":"e_1_2_3_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1964.tb14213.x"},{"key":"e_1_2_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)65721-7"},{"key":"e_1_2_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(67)11054-9"},{"key":"e_1_2_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2795(67)90078-5"},{"key":"e_1_2_3_15_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1190805f"},{"key":"e_1_2_3_16_2","first-page":"328","volume":"11","author":"Hirs C. H. W.","year":"1967","journal-title":"Methods Enzymol."},{"key":"e_1_2_3_17_2","first-page":"409","volume":"11","author":"Gray W. R.","year":"1967","journal-title":"Methods Enzymol."},{"key":"e_1_2_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/211591a0"},{"key":"e_1_2_3_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/181996b0"},{"key":"e_1_2_3_20_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1020258"},{"key":"e_1_2_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2795(70)90101-7"},{"key":"e_1_2_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)97349-7"},{"key":"e_1_2_3_23_2","unstructured":"Dayhoff M. O.&Eck R. V. eds (1968) inAtlas of Protein Sequence and Structure."},{"key":"e_1_2_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(71)90364-X"},{"key":"e_1_2_3_25_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1967.0034"},{"key":"e_1_2_3_26_2","doi-asserted-by":"crossref","first-page":"1909","DOI":"10.1016\/S0021-9258(18)62395-6","volume":"246","author":"Kronman M. J.","year":"1971","journal-title":"J. Biol. Chem."},{"key":"e_1_2_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(70)90039-2"},{"key":"e_1_2_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(69)90487-2"},{"key":"e_1_2_3_29_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00807a024"},{"key":"e_1_2_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2795(71)90158-9"},{"key":"e_1_2_3_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/219131a0"},{"key":"e_1_2_3_32_2","doi-asserted-by":"crossref","first-page":"1909","DOI":"10.1016\/S0021-9258(18)62395-6","volume":"246","author":"Kronman M. J.","year":"1971","journal-title":"J. Biol. Chem."},{"key":"e_1_2_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2795(70)90061-9"},{"key":"e_1_2_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2795(68)90284-5"},{"key":"e_1_2_3_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.62.1.163"}],"container-title":["European Journal of Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1432-1033.1972.tb01812.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1432-1033.1972.tb01812.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T12:07:39Z","timestamp":1700741259000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1111\/j.1432-1033.1972.tb01812.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1972,5]]},"references-count":34,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1972,5]]}},"alternative-id":["10.1111\/j.1432-1033.1972.tb01812.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1432-1033.1972.tb01812.x","archive":["Portico"],"relation":{},"ISSN":["0014-2956","1432-1033"],"issn-type":[{"value":"0014-2956","type":"print"},{"value":"1432-1033","type":"electronic"}],"subject":[],"published":{"date-parts":[[1972,5]]}}}