{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T13:45:36Z","timestamp":1756993536044},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,6,28]],"date-time":"2008-06-28T00:00:00Z","timestamp":1214611200000},"content-version":"vor","delay-in-days":11836,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[1976,2]]},"abstract":"<jats:p>Poly(A)\u2010containing RNAs from cytoplasm and nuclei of adult <jats:italic>Xenopus<\/jats:italic> liver cells are compared.<\/jats:p><jats:p>After denaturation of the RNA by dimethylsulfoxide the average molecule of nuclear poly(A)containing RNA has a sedimentation value of 28 S whereas the cytoplasmic poly(A)\u2010containing RNA sediments slightly ahead of 18 S.<\/jats:p><jats:p>To compare the complexity of cytoplasmic and nuclear poly(A)\u2010containing RNA, complementary DNA (cDNA) transcribed on either cytoplasmic or nuclear RNA is hybridized to the RNA used as a template. The hybridization kinetics suggest a higher complexity of the nuclear RNA compared to the cytoplasmic fraction. Direct evidence of a higher complexity of nuclear poly(A)\u2010containing RNA is shown by the fact that 30% of the nuclear cDNA fails to hybridize with cytoplasmic poly(A)containing RNA. An attempt to isolate a specific probe for this nucleus\u2010restricted poly(A)\u2010containing RNA reveals that more than 10<jats:sup>4<\/jats:sup> different nuclear RNA sequences adjacent to the poly(A) do not get into the cytoplasts. We conclude that a poly(A) on a nuclear RNA does not ensure the transport of the adjacent sequence to the cytoplasm.<\/jats:p>","DOI":"10.1111\/j.1432-1033.1976.tb10174.x","type":"journal-article","created":{"date-parts":[[2005,3,3]],"date-time":"2005-03-03T16:21:39Z","timestamp":1109866899000},"page":"417-423","source":"Crossref","is-referenced-by-count":42,"title":["Comparison of Cytoplasmic and Nuclear Poly(A)\u2010Containing RNA Sequences in <i>Xenopus<\/i> Liver Cells"],"prefix":"10.1111","volume":"62","author":[{"given":"Gerhart U.","family":"RYFFEL","sequence":"first","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2008,6,28]]},"reference":[{"key":"e_1_2_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(75)90128-2"},{"key":"e_1_2_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(75)90113-0"},{"key":"e_1_2_2_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.181.4106.1215"},{"key":"e_1_2_2_5_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.64.2.269"},{"key":"e_1_2_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(74)90593-2"},{"key":"e_1_2_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.1.389"},{"key":"e_1_2_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.70.4.1122"},{"key":"e_1_2_2_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.71.12.5009"},{"key":"e_1_2_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(74)90127-5"},{"key":"e_1_2_2_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.71.11.4327"},{"key":"e_1_2_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/254356a0"},{"key":"e_1_2_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(75)90104-X"},{"key":"e_1_2_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(75)90118-X"},{"key":"e_1_2_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(74)90461-8"},{"key":"e_1_2_2_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00754a027"},{"key":"e_1_2_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(75)90217-X"},{"key":"e_1_2_2_18_2","first-page":"413","volume-title":"Fundamental Techniques in Virology","author":"Scherrer K.","year":"1969"},{"key":"e_1_2_2_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00678a006"},{"key":"e_1_2_2_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00755a019"},{"key":"e_1_2_2_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/250199a0"},{"key":"e_1_2_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0045-6039(74)90005-0"},{"key":"e_1_2_2_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00682a026"},{"key":"e_1_2_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(75)90203-X"},{"key":"e_1_2_2_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.71.9.3746"},{"key":"e_1_2_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(74)90033-6"},{"key":"e_1_2_2_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00678a007"}],"container-title":["European Journal of Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1432-1033.1976.tb10174.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1432-1033.1976.tb10174.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T17:17:59Z","timestamp":1700759879000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1111\/j.1432-1033.1976.tb10174.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1976,2]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1976,2]]}},"alternative-id":["10.1111\/j.1432-1033.1976.tb10174.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1432-1033.1976.tb10174.x","archive":["Portico"],"relation":{},"ISSN":["0014-2956","1432-1033"],"issn-type":[{"value":"0014-2956","type":"print"},{"value":"1432-1033","type":"electronic"}],"subject":[],"published":{"date-parts":[[1976,2]]}}}