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More importantly, the assay measures the initial step(s) of proteolytic attack, making it specially suitable for the examination of the controlling factors that regulate proteolytic degradation and\/or the detection of \u201cspecific\u201d proteinases. The method allows the simple determination of the general parameters of enzyme action, <jats:italic>V<\/jats:italic> and <jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub>, using proteins, i.e. the physiological substrates of the proteinases. The more appropriate proteins to be used as substrates are the N\u2010amino\u2010terminally blocked ones. Many proteins fulfill this requirement. If the particular protein whose degradation has to be studied lacks this modification, three different approaches can be used to study its degradation: (a)the accumulation of N\u2010amino termini in excess over that of the intact substrate; (b)a gel filtration\/continuous method and (c) the chemical blockage of its amino groups. The particular advantages of each of these approaches are discussed.<\/jats:p>","DOI":"10.1111\/j.1432-1033.1979.tb13252.x","type":"journal-article","created":{"date-parts":[[2005,3,3]],"date-time":"2005-03-03T20:07:56Z","timestamp":1109880476000},"page":"253-260","source":"Crossref","is-referenced-by-count":34,"title":["A Fluorescamine\u2010Based Sensitive Method for the Assay of Proteinases, Capable of Detecting the Initial Cleavage Steps of a Protein"],"prefix":"10.1111","volume":"99","author":[{"given":"Rafael","family":"GARESSE","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 V.","family":"CASTELL","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen G.","family":"VALLEJO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"MARCO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,6,28]]},"reference":[{"key":"e_1_2_2_2_2","volume-title":"Cold Spring Harbor Symposium Proteases and Biological Control","author":"Reich E.","year":"1975"},{"key":"e_1_2_2_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.44.070175.004015"},{"key":"e_1_2_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.11.3825"},{"key":"e_1_2_2_5_2","volume-title":"Proteolysis and Physiological Regulation","author":"Ribbons D. 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