{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T02:28:26Z","timestamp":1768876106650,"version":"3.49.0"},"reference-count":55,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,6,23]],"date-time":"2004-06-23T00:00:00Z","timestamp":1087948800000},"content-version":"vor","delay-in-days":2396,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European Journal of Biochemistry"],"published-print":{"date-parts":[[1997,12]]},"abstract":"<jats:p>This communication demonstrates that proteins in the family of myrosinase\u2010binding proteins (MBP) present in seeds of <jats:italic>Brassica napus<\/jats:italic> possess lectin activity, binding most efficiently to p\u2010aminophenyl \u03b1 L\u2010D\u2010mannopyranoside\u2013agarose, and to some extent to N\u2010acetylglucosamine\u2013agarose. A cDNA encoding a vegetatively expressed, wound\u2010inducible counterpart to these seed MBP was isolated and characterised. Upon wounding, this MBP transcript accumulated in old and young leaves, and was systemically expressed in the young plant. Additionally, the wound\u2010induced MBP transcript increased in abundance after treating the young plants with methyl jasmonate (MeJA), jasmonic acid (JA) or abscisic acid (ABA), and to some extent in response to the ethylene precursor 1\u2010aminocyclopropane\u20101\u2010carboxylic acid. Expression induced by wounding, ABA or JA was antagonised by simultaneous feeding of the plants with salicylic acid. MBP polypeptides accumulated in MeJA\u2010treated plants. The myrosinases redistributed from the soluble fraction into the insoluble fraction of a tissue extract after induction. The most abundant MBP (94 kDa) partitioned in the insoluble fraction, while two larger MBP (103 kDa and 108 kDa) were present only in the soluble fraction of extracts obtained from the control or MeJA\u2010treated plant tissues.<\/jats:p>","DOI":"10.1111\/j.1432-1033.1997.00680.x","type":"journal-article","created":{"date-parts":[[2004,6,23]],"date-time":"2004-06-23T15:10:28Z","timestamp":1088003428000},"page":"680-688","source":"Crossref","is-referenced-by-count":56,"title":["The Myrosinase\u2010Binding Protein from <i>Brassica Napus<\/i> Seeds Possesses lectin Activity and has a Highly Similar Vegetatively Expressed Wound\u2010Inducible Counterpart"],"prefix":"10.1111","volume":"250","author":[{"given":"Jan","family":"Taipalensuu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanna","family":"Eriksson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars","family":"Rask","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,6,23]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00020173"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.111.2.525"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(73)80372-3"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(92)83609-3"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(00)94752-6"},{"key":"e_1_2_2_7_1","first-page":"18","article-title":"Physiology of lightly processed fruits and vegetables","volume":"30","author":"Brecht J. K.","year":"1995","journal-title":"Hortic. Sci."},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.19.7633"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.2740560104"},{"key":"e_1_2_2_10_1","first-page":"1","article-title":"Lectins, lectin genes, and their role in plant defense","volume":"3","author":"Chrispeels M. J.","year":"1991","journal-title":"Plant Cell"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02383553"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-2452(96)81488-6"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.108.4.1741"},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(94)00653-B"},{"key":"e_1_2_2_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-7348.1991.tb05648.x"},{"key":"e_1_2_2_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/360183a0"},{"key":"e_1_2_2_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1570-7458.1994.tb01796.x"},{"key":"e_1_2_2_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00037015"},{"key":"e_1_2_2_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00202596"},{"key":"e_1_2_2_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-9452(92)90077-Y"},{"key":"e_1_2_2_21_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.98.3.995"},{"key":"e_1_2_2_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-7348.1994.tb04158.x"},{"key":"e_1_2_2_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.2740350402"},{"key":"e_1_2_2_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-7348.1995.tb05371.x"},{"key":"e_1_2_2_25_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.112.2.853"},{"key":"e_1_2_2_26_1","first-page":"1157","article-title":"General roles of abscisic and jasmonic acids in gene activation as a result of mechanical wounding","volume":"4","author":"Hildmann T.","year":"1992","journal-title":"Plant Cell"},{"key":"e_1_2_2_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-9452(92)90112-Y"},{"key":"e_1_2_2_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.1991.tb00076.x"},{"key":"e_1_2_2_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/45.9.1343"},{"key":"e_1_2_2_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-7348.1991.tb06099.x"},{"key":"e_1_2_2_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00224191"},{"key":"e_1_2_2_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00195196"},{"key":"e_1_2_2_33_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.103.3.703"},{"key":"e_1_2_2_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1990.tb19465.x"},{"key":"e_1_2_2_35_1","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/B978-0-12-597183-6.50009-7","volume-title":"Glucosinolates: chemistry and ecology. In Herbivores, their interaction with secondary plant metabolites","author":"Louda S.","year":"1991"},{"key":"e_1_2_2_36_1","first-page":"826","article-title":"Review of analysis of glucosinolates","volume":"66","author":"McGregor D. I.","year":"1983","journal-title":"J. Assoc. Off. Anal. Chem."},{"key":"e_1_2_2_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00023913"},{"key":"e_1_2_2_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00240903"},{"key":"e_1_2_2_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00620062"},{"key":"e_1_2_2_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00398968"},{"key":"e_1_2_2_41_1","first-page":"1197","article-title":"JIPs and RIPs: the regulation of plant gene expression by jasmonates in response to environmental cues and pathogens","volume":"6","author":"Reinbothe S.","year":"1994","journal-title":"Plant Cell (Heidelb.)"},{"key":"e_1_2_2_42_1","volume-title":"Molecular cloning: a laboratory manual","author":"Sambrook J.","year":"1989"},{"key":"e_1_2_2_43_1","doi-asserted-by":"publisher","DOI":"10.4141\/cjps84-011"},{"key":"e_1_2_2_44_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pp.44.060193.003033"},{"key":"e_1_2_2_45_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/44.5.963"},{"key":"e_1_2_2_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-3912-4_9"},{"key":"e_1_2_2_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00339718"},{"key":"e_1_2_2_48_1","volume-title":"Brassica napus, PhD thesis, Swedish University of Agricultural Sciences","author":"Taipalensuu J.","year":"1996"},{"key":"e_1_2_2_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1997.00963.x"},{"key":"e_1_2_2_50_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.110.2.483"},{"key":"e_1_2_2_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1997.t01-1-00605.x"},{"key":"e_1_2_2_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01322721"},{"key":"e_1_2_2_53_1","first-page":"103","volume-title":"Toxic constituents of plant foodstuffs","author":"Tookey H. L.","year":"1980"},{"key":"e_1_2_2_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0958-1669(96)80116-9"},{"key":"e_1_2_2_55_1","doi-asserted-by":"publisher","DOI":"10.2307\/3869886"},{"key":"e_1_2_2_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00382075"}],"container-title":["European Journal of Biochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1432-1033.1997.00680.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1432-1033.1997.00680.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T12:59:59Z","timestamp":1700744399000},"score":1,"resource":{"primary":{"URL":"https:\/\/febs.onlinelibrary.wiley.com\/doi\/10.1111\/j.1432-1033.1997.00680.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,12]]},"references-count":55,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1997,12]]}},"alternative-id":["10.1111\/j.1432-1033.1997.00680.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1432-1033.1997.00680.x","archive":["Portico"],"relation":{},"ISSN":["0014-2956","1432-1033"],"issn-type":[{"value":"0014-2956","type":"print"},{"value":"1432-1033","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,12]]}}}