{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T18:24:42Z","timestamp":1758392682435},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T00:00:00Z","timestamp":1160006400000},"content-version":"vor","delay-in-days":10353,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[1978,6]]},"abstract":"<jats:p><jats:bold>Abstract\u2014<\/jats:bold> Microtubular protein was isolated from rat forebrain by biochemical purification (ammonium sulfate precipitation followed by DEAE cellulose chromatography) or by two cycles of aggregation\u2010disaggregation. The protein subunit structure was examined on two\u2010dimensional electrophoretograms: first dimension, urea isoelectric focusing gel; second dimension, sodium dodecyl sulfate exponential acrylamide slab gel. Two forms of \u03b1 tubulin were separated in the second dimension on the basis of different rates of migration (\u03b1 and \u03b1<jats:sub>2<\/jats:sub>). Each of these species was further separated into at least three forms with different isoelectric points. \u03b2 Tubulin was separated into a minor species (BI) and a major species \u03b2<jats:sub>2<\/jats:sub>). Multiple subunits were observed using protein from either purification method and in a two\u2010dimensional electrophoretogram of total supernatant proteins from rat brain. Separation and visualization of multiple forms of \u03b1 and \u03b2 tubulin is consistent with reports that provide evidence for post\u2010translational modification of these proteins.<\/jats:p>","DOI":"10.1111\/j.1471-4159.1978.tb10475.x","type":"journal-article","created":{"date-parts":[[2006,10,5]],"date-time":"2006-10-05T08:01:27Z","timestamp":1160035287000},"page":"1431-1440","source":"Crossref","is-referenced-by-count":76,"title":["CHARACTERIZATION OF MULTIPLE FORMS OF BRAIN TUBULIN SUBUNITS"],"prefix":"10.1111","volume":"30","author":[{"given":"Charles A.","family":"Marotta","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jame L.","family":"Harris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey M.","family":"Gilbert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,10,5]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00648a026"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(75)81027-1"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1975.tb02435.x"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.69.2.301"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1975.tb19196.x"},{"key":"e_1_2_2_7_1","first-page":"152","volume":"33","author":"Bryan J.","year":"1974","journal-title":"Fedn Proc. 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