{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:06:34Z","timestamp":1762160794847},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,10,20]],"date-time":"2009-10-20T00:00:00Z","timestamp":1255996800000},"content-version":"vor","delay-in-days":17271,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[1962,7,8]]},"abstract":"<jats:p>Patients who have been sensitized to leukocytes by transfusions and pregnancy are subject to \u201cpyrogenic\u201d reactions of varying severity following the administration of whole blood. Present methods for preparing buffy coat\u2010poor blood utilize differential centrifugation or sedimentation in the presence of dextran. They are time consuming and not uniformly dependable.<\/jats:p><jats:p>The procedure described removes the granulocytes from heparinized whole blood by passing it over a column of Nylon fibers in ten to 15 minutes. Only 5 per cent of the donation is retained in the column and the white blood counts of the final product are 500\u20103,100\/cu. mm. with 95 to 100 per cent lymphocytes. Cr<jats:sup>51<\/jats:sup> survival studies suggest that these preparations withstand storage satisfactorily for 14 to 21 days.<\/jats:p><jats:p>Our experience with the transfusion of 82 units of this product and the relative importance of lymphocytes and granulocytes in leukoagglutination are discussed.<\/jats:p>","DOI":"10.1111\/j.1537-2995.1962.tb00228.x","type":"journal-article","created":{"date-parts":[[2009,10,20]],"date-time":"2009-10-20T15:42:38Z","timestamp":1256053358000},"page":"221-229","source":"Crossref","is-referenced-by-count":158,"title":["A New Method for Preparing Buffy Coat\u2010Poor Blood"],"prefix":"10.1111","volume":"2","author":[{"given":"Tibor J.","family":"Greenwalt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mieczyslaw","family":"Gajewski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James L.","family":"McKenna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,10,20]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"5","volume-title":"Standard Values in Blood","author":"Albritton E. C.","year":"1952"},{"key":"e_1_2_1_3_2","unstructured":"Andre R. B.DreyfusandC. H.Salmon:Antileukocytic isoantibodies (lymphocytes and polynuclears) in polytransfused patients. Sixth Congr. Internat. Soc. Hematol. Boston 1956. New York Grune and Stratton 1958 p.900."},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1182\/blood.V16.6.1745.1745","article-title":"Familial neonatal neutropenia with maternal leukocyte antibodies","volume":"16","author":"Braun E. H.","year":"1960","journal-title":"Blood."},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1950.3.6.365"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1957.02980250053013"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1182\/blood.V17.2.139.139","article-title":"The antigenicity of normal and leukemic human leukocytes","volume":"17","author":"Brittingham T. E.","year":"1961","journal-title":"Blood."},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/31.5.373"},{"key":"e_1_2_1_9_2","first-page":"190","article-title":"Leuko\u2010agglutinins IV. Leuko\u2010agglutinins and blood transfusion","volume":"4","author":"Dausset J.","year":"1954","journal-title":"Vox Sang."},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1957.tb03700.x"},{"key":"e_1_2_1_11_2","unstructured":"Dausset J. H.QuijadaandH.Brecy:New facts in immunohematology of the leukocytes: antigenic differences of normal and leukemic leukocytes. Sixth Congr. Internat. Soc. Hematol. Boston 1956. New York Grune and Stratton 1958 p.834."},{"key":"e_1_2_1_12_2","first-page":"28","article-title":"A simple method to separate lymphocytes from the blood","volume":"56","author":"Fichtelius K.","year":"1950","journal-title":"Acta Soc. Med. Upsal."},{"key":"e_1_2_1_13_2","first-page":"71","article-title":"Adhesive properties of human leukocytes on a glass bead column","volume":"20","author":"Garvin J. E.","year":"1961","journal-title":"Fed. Proc."},{"key":"e_1_2_1_14_2","unstructured":"Greenwalt T. J.andV. E.Ayers: Unpublished observations."},{"key":"e_1_2_1_15_2","first-page":"2167","article-title":"Leuko\u2010agglutinins in transfusion practice","volume":"10","author":"Greenwalt T. J.","year":"1959","journal-title":"Amer. Pract. & Dig. of Treat."},{"key":"e_1_2_1_16_2","unstructured":"Greenwalt T. J.andM.Gajewski: Unpublished observations."},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1093\/ajcp\/33.4_ts.358","article-title":"Gum acacia for preparing suspensions of leukocytes","volume":"33","author":"Greenwalt T. J.","year":"1960","journal-title":"Am. J. Clin. Path."},{"key":"e_1_2_1_18_2","first-page":"142","article-title":"The preparation of iso\u2010osmotic phosphate buffer solutions","volume":"55","author":"Hendry E. B.","year":"1948","journal-title":"Edinb. Med. J."},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1956.tb06719.x"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-102-25237"},{"key":"e_1_2_1_21_2","first-page":"95","volume-title":"Leukocyte Agglutinins","author":"Killmann S.","year":"1960"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V15.2.236.236"},{"key":"e_1_2_1_23_2","unstructured":"Minimum Requirements: Citrated Whole Blood (Human). U.S. Department of Health Education and Welfare March 15 1955."},{"key":"e_1_2_1_24_2","first-page":"55","volume-title":"Blood Transfusion in Clinical Medicine","author":"Mollison P. L.","year":"1956"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1957.tb03698.x"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1957.00260040087010"},{"key":"e_1_2_1_27_2","unstructured":"Payne R.:Personal communication."},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V11.5.436.436"},{"key":"e_1_2_1_29_2","volume-title":"Standards for a Blood Transfusion Service","year":"1960"},{"key":"e_1_2_1_30_2","first-page":"394","article-title":"Identification of the type of blood\u2010cell responsible for the gratt\u2010versus\u2010host reaction in chicks","volume":"7","author":"Terasaki P. I.","year":"1959","journal-title":"J. Embryol. Exp. Morph."},{"key":"e_1_2_1_31_2","first-page":"130","volume-title":"Sedimentation technics in Methods in Medical Research","author":"Tullis J. L.","year":"1958"},{"key":"e_1_2_1_32_2","first-page":"64","article-title":"Leukozyten\u2010Antik\u00f6rper als Urasche von Transfusions Zwischenf\u00e4llen","volume":"9","author":"Loghem J. J.","year":"1959","journal-title":"Bibl. Haemat."},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1957.tb03701.x"},{"key":"e_1_2_1_34_2","first-page":"182","volume-title":"Leukocyte Antigens and Antibodies","author":"Walford R. L.","year":"1960"},{"key":"e_1_2_1_35_2","first-page":"231","volume-title":"Clinical Hematology","author":"Wintrobe M. M.","year":"1956"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1537-2995.1962.tb00228.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1537-2995.1962.tb00228.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T19:36:10Z","timestamp":1698867370000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1537-2995.1962.tb00228.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1962,7,8]]},"references-count":34,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1962,7,8]]}},"alternative-id":["10.1111\/j.1537-2995.1962.tb00228.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1537-2995.1962.tb00228.x","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[1962,7,8]]}}}