{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:45:29Z","timestamp":1762271129814},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,4,30]],"date-time":"2007-04-30T00:00:00Z","timestamp":1177891200000},"content-version":"vor","delay-in-days":13786,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Protozoology"],"published-print":{"date-parts":[[1969,8]]},"abstract":"<jats:p><jats:bold>SYNOPSIS. <\/jats:bold>  <jats:italic>Tetrahymena pyriformis<\/jats:italic> contains a potent system of lipolytic enzymes which is activated by disruption of the cells. The extent to which endogenous lipids of the homogenate are degraded depends upon the age of the culture and the strain of <jats:italic>T. pyriformis<\/jats:italic> used. In their most active form, the enzymes can hydrolyze nearly 60% of the endogenous phospholipids within one hour at room temperature. The products of this reaction are probably responsible for the frequently reported instability of <jats:italic>Tetrahymena<\/jats:italic> enzyme systems <jats:italic>in vitro.<\/jats:italic> The use of inhibitors and the careful choice of culture conditions can reduce lipid degradation to a negligible level.<\/jats:p>","DOI":"10.1111\/j.1550-7408.1969.tb02287.x","type":"journal-article","created":{"date-parts":[[2007,5,14]],"date-time":"2007-05-14T00:34:19Z","timestamp":1179102859000},"page":"397-400","source":"Crossref","is-referenced-by-count":12,"title":["The Properties of an Enzyme System Degrading Endogenous Phospholipids of <i>Tetrahymena pyriformis<\/i>"],"prefix":"10.1111","volume":"16","author":[{"suffix":"JR.","given":"GUY A.","family":"THOMPSON","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,4,30]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"3537","DOI":"10.1016\/S0021-9258(18)97757-4","article-title":"Oxidation of reduced diphosphopyridine nucleotide by Tetrahymena pyriformis preparations.","volume":"239","author":"Rahat M.","year":"1964","journal-title":"J. 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