{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T05:53:02Z","timestamp":1790920382834,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP23H00394"],"award-info":[{"award-number":["JP23H00394"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP23H04696"],"award-info":[{"award-number":["JP23H04696"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP26K23190"],"award-info":[{"award-number":["JP26K23190"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"publisher","award":["JPMJFR233E"],"award-info":[{"award-number":["JPMJFR233E"]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007449","name":"Takeda Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007449","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007451","name":"Cell Science Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007451","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Aging Cell"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Regenerative capacity varies widely across animals, yet aging is often accompanied by declining tissue homeostasis and regenerative potential. In many species, regeneration of complex structures relies on epimorphic programs that form a blastema, coordinating cell proliferation and pattern reorganization after injury. Although aging\u2010associated regeneration defects have been documented in several bilaterian models, how aging shapes regeneration in early\u2010branching metazoans with robust regenerative abilities remains unclear. Here, we investigate aging and regeneration in the medusa stage of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Cladonema pacificum<\/jats:italic>\n                    <\/jats:styled-content>\n                    , a cnidarian that retains high regenerative capacity within a finite lifespan. We show that aging in\n                    <jats:italic>Cladonema<\/jats:italic>\n                    medusae is accompanied by progressive morphological and functional deterioration, including shrinkage of the umbrella and manubrium, tentacle shortening, and reduced reproductive output, along with increased DNA damage. At the cellular level, aging is associated with depletion of differentiated cell populations, including nematocytes and neurons, as well as reductions in stem cell\u2010associated populations and proliferative activity in the tentacle bulb. Consistent with these changes, tentacle regeneration is markedly impaired in aged medusae and is characterized by delayed wound closure, defective blastema formation and incomplete functional recovery. Together, our findings indicate that aging disrupts both tissue homeostasis and blastema\u2010mediated regeneration in\n                    <jats:italic>Cladonema<\/jats:italic>\n                    medusae, establishing a tractable model for studying aging\u2013regeneration interactions and supporting the view that aging imposes broadly shared constraints on regenerative systems across metazoans.\n                  <\/jats:p>","DOI":"10.1111\/acel.70727","type":"journal-article","created":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T05:00:18Z","timestamp":1790917218000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Aging Disrupts Tissue Homeostasis and Constrains Blastema\u2010Mediated Regeneration in the\n                    <i>Cladonema<\/i>\n                    Medusa"],"prefix":"10.1111","volume":"25","author":[{"given":"Ren","family":"Kanehisa","sequence":"first","affiliation":[{"name":"Laboratory of Genetics, Graduate School of Pharmaceutical Sciences The University of Tokyo  Bunky\u014d Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroko","family":"Nakatani","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, Graduate School of Pharmaceutical Sciences The University of Tokyo  Bunky\u014d Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3925-4011","authenticated-orcid":false,"given":"Sho","family":"Takatori","sequence":"additional","affiliation":[{"name":"Laboratory of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences The University of Tokyo  Bunky\u014d Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0075-5943","authenticated-orcid":false,"given":"Taisuke","family":"Tomita","sequence":"additional","affiliation":[{"name":"Laboratory of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences The University of Tokyo  Bunky\u014d Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayuki","family":"Miura","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, Graduate School of Pharmaceutical Sciences The University of Tokyo  Bunky\u014d Tokyo Japan"},{"name":"Laboratory for Cell Vigor Regulation National Institute for Basic Biology  Okazaki Aichi Japan"},{"name":"Basic Biology Program The Graduate University for Advanced Studies, SOKENDAI  Okazaki Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1823-5989","authenticated-orcid":false,"given":"Yu\u2010ichiro","family":"Nakajima","sequence":"additional","affiliation":[{"name":"Laboratory of Genetics, Graduate School of Pharmaceutical Sciences The University of Tokyo  Bunky\u014d Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,1]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.203083"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.210126"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.200800183"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.05506"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-022-00510-w"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abh1683"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43587-025-00847-9"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.12.023"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.689463"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.240111yl"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2017.01.019"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40851-019-0124-4"},{"key":"e_1_2_11_14_1","doi-asserted-by":"crossref","unstructured":"Fujita S. R.Kanehisa R.Nakajima et\u00a0al.2026.\u201cDivision of Labor Between ERK and Notch Signaling Coordinates Distinct Stem Cell Populations During Jellyfish Tentacle Regeneration.\u201dhttps:\/\/doi.org\/10.64898\/2026.03.08.710341.","DOI":"10.64898\/2026.03.08.710341"},{"key":"e_1_2_11_15_1","article-title":"Fluorescent In\u00a0Situ Hybridization and 5\u2010Ethynyl\u20102\u2032\u2010Deoxyuridine Labeling for Stem\u2010Like Cells in the Hydrozoan Jellyfish \n                     Cladonema pacificum","volume":"186","author":"Fujita S.","year":"2022","journal-title":"Journal of Visualized 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