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Notably, high levels of estradiol (E2) are associated with low sperm production and quality. Sertoli cells (SCs) are responsible for spermatogenesis maintenance and are major targets for the hormonal signalling that regulates this complex process.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In this study, we used primary cultures of human SCs and studied the localisation, expression and functionality of the Na<jats:sup>+<\/jats:sup>\u2010dependent HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>transporters by confocal microscopy, immunoblot, epifluorescence and voltage clamp after 24 h of exposure to E2 (100 nM). All studied transporters were identified in human SCs. In E2\u2010treated human SCs, there was an increase in NBCn1, NBCe1 and NDCBE protein levels, as well as an increase in intracellular pH and a decrease in transcellular transport.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We report an association between increased levels of E2 and the expression\/function of Na<jats:sup>+<\/jats:sup>\u2010dependent HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup>transporters in human SCs. Our results provide new evidence on the mechanisms by which E2 can regulate SCs physiology and consequently spermatogenesis. These mechanisms may have an influence on male reproductive potential and help to explain male infertility conditions associated with estrogen deregulation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Significance<\/jats:title><jats:p>Exposure to E2 increased human SCs intracellular pH. E2 is a modulator of ionic transcellular transport in human SCs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/boc.201500094","type":"journal-article","created":{"date-parts":[[2016,2,18]],"date-time":"2016-02-18T12:19:36Z","timestamp":1455797976000},"page":"179-188","source":"Crossref","is-referenced-by-count":29,"title":["Estradiol modulates Na<sup>+<\/sup>\u2010dependent HCO<sub>3<\/sub><sup>\u2212<\/sup>transporters altering intracellular pH and ion transport in human Sertoli cells: A role on male fertility?"],"prefix":"10.1111","volume":"108","author":[{"given":"Raquel L.","family":"Bernardino","sequence":"first","affiliation":[{"name":"Department of Microscopy Laboratory of Cell Biology Institute of Biomedical Sciences Abel Salazar (ICBAS) and Unit for Multidisciplinary Research in Biomedicine (UMIB) University of Porto Porto Portugal"}]},{"given":"Ana R.","family":"Costa","sequence":"additional","affiliation":[{"name":"CICS\u2013UBI\u2013Health Sciences Research Centre University of Beira Interior Covilh\u00e3 Portugal"}]},{"given":"Ana D.","family":"Martins","sequence":"additional","affiliation":[{"name":"Department of Microscopy Laboratory of Cell Biology Institute of Biomedical Sciences Abel Salazar (ICBAS) and Unit for Multidisciplinary Research in Biomedicine (UMIB) University of Porto Porto Portugal"},{"name":"CICS\u2013UBI\u2013Health Sciences Research Centre University of Beira Interior Covilh\u00e3 Portugal"}]},{"given":"Joaquina","family":"Silva","sequence":"additional","affiliation":[{"name":"Centre for Reproductive Genetics Prof. Alberto Barros Porto Portugal"}]},{"given":"Alberto","family":"Barros","sequence":"additional","affiliation":[{"name":"Centre for Reproductive Genetics Prof. Alberto Barros Porto Portugal"},{"name":"Department of Genetics Faculty of Medicine University of Porto Porto Portugal"},{"name":"I3S, Institute for Innovation and Health Research University of Porto Porto Portugal"}]},{"given":"M\u00e1rio","family":"Sousa","sequence":"additional","affiliation":[{"name":"Department of Microscopy Laboratory of Cell Biology Institute of Biomedical Sciences Abel Salazar (ICBAS) and Unit for Multidisciplinary Research in Biomedicine (UMIB) University of Porto Porto Portugal"},{"name":"Centre for Reproductive Genetics Prof. Alberto Barros Porto Portugal"}]},{"given":"Ros\u00e1lia","family":"S\u00e1","sequence":"additional","affiliation":[{"name":"Department of Microscopy Laboratory of Cell Biology Institute of Biomedical Sciences Abel Salazar (ICBAS) and Unit for Multidisciplinary Research in Biomedicine (UMIB) University of Porto Porto Portugal"}]},{"given":"Marco G.","family":"Alves","sequence":"additional","affiliation":[{"name":"CICS\u2013UBI\u2013Health Sciences Research Centre University of Beira Interior Covilh\u00e3 Portugal"}]},{"given":"Pedro F.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Microscopy Laboratory 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