{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T23:13:21Z","timestamp":1762298001010},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,7,16]],"date-time":"2013-07-16T00:00:00Z","timestamp":1373932800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["EPPO Bulletin"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:p><jats:italic><jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>ibberella circinata<\/jats:italic> (anamorph <jats:italic><jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>usarium circinatum<\/jats:italic>) is a harmful fungus infecting pine trees that has been subject to <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>uropean Union emergency phytosanitary measures since 2007. This fungus is spread over long distances primarily via pine seeds, consequently reliable detection tools and methods are needed to test pine seed lots imported and marketed in the EU. Several detection methods are available in the scientific literature but very limited validation data is available. For that purpose, one <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>uropean <jats:styled-content style=\"fixed-case\">EUPHRESCO<\/jats:styled-content> project called \u2018<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>ibcir\u2010<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>iagseed\u2019 was launched, involving 10 countries. This project aimed to select and validate one or more diagnostic procedures targeting this pest via inter\u2010laboratory tests, as well as a suitable sampling method. Performance criteria were calculated for three procedures chosen by participants: isolation followed by morphological characterization, isolation followed by conventional <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and biological enrichment followed by real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. The results showed that all three procedures provided acceptable performance criteria but also demonstrated that none of them could generate 100% of correct results. Regarding the sampling issue, the <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>ational <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>lant <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>rotection <jats:styled-content style=\"fixed-case\">O<\/jats:styled-content>rganizations consultation enabled an agreement to be reached on the number of seeds to be tested: 400 per sample, for a 95% confidence level that an infection of at least 1% will be detected.<\/jats:p>","DOI":"10.1111\/epp.12037","type":"journal-article","created":{"date-parts":[[2013,7,16]],"date-time":"2013-07-16T17:58:59Z","timestamp":1373997539000},"page":"267-275","source":"Crossref","is-referenced-by-count":4,"title":["Test performance study of diagnostic procedures for identification and detection of <i><scp>G<\/scp>ibberella circinata<\/i> in pine seeds in the framework of a <scp>EUPHRESCO<\/scp> project"],"prefix":"10.1111","volume":"43","author":[{"given":"R.","family":"Ioos","sequence":"first","affiliation":[{"name":"Anses Laboratoire de la Sant\u00e9 des V\u00e9g\u00e9taux Unit\u00e9 de mycologie Domaine de Pix\u00e9r\u00e9court 54220 Malz\u00e9ville France"}]},{"given":"T.","family":"Annesi","sequence":"additional","affiliation":[{"name":"Consiglio per la Ricerca e la Sperimentazione in Agricoltura Centro di Ricerca per la Patologia Vegetale Via C.G. Bertero 22 I\u201000156 Rome Italy"}]},{"given":"C.","family":"Fourrier","sequence":"additional","affiliation":[{"name":"Anses Laboratoire de la Sant\u00e9 des V\u00e9g\u00e9taux Unit\u00e9 de mycologie Domaine de Pix\u00e9r\u00e9court 54220 Malz\u00e9ville France"}]},{"given":"C.","family":"Saurat","sequence":"additional","affiliation":[{"name":"Anses Laboratoire de la Sant\u00e9 des V\u00e9g\u00e9taux Unit\u00e9 de mycologie Domaine de Pix\u00e9r\u00e9court 54220 Malz\u00e9ville France"}]},{"given":"A.","family":"Chandelier","sequence":"additional","affiliation":[{"name":"Centre Wallon de Recherches Agronomiques Unit\u00e9 Biologie des Nuisibles et Biovigilance Rue de Liroux 4, 5030 Gembloux Belgium"}]},{"given":"S.","family":"Inghelbrecht","sequence":"additional","affiliation":[{"name":"Plant Sciences Unit Crop Protection Institute for Agricultural and Fisheries Research (ILVO) Burgemeester Van Gansberghelaan 96 bus 2 9820 Merelbeke Belgium"}]},{"given":"E. L. F.","family":"Diogo","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Investiga\u00e7\u00e3o Agr\u00e1ria e Veterin\u00e1ria, I.P. Unidade Estrat\u00e9gica de Investiga\u00e7\u00e3o e Servi\u00e7os de Sistemas Agr\u00e1rios e Florestais e Sanidade Vegetal Av. da Rep\u00fablica Quinta do Marqu\u00eas 2780\u2010159 Oeiras Portugal"}]},{"given":"A.\u2010M.","family":"P\u00e9rez\u2010Sierra","sequence":"additional","affiliation":[{"name":"Grupo de Investigaci\u00f3n en Hongos Fitopat\u00f3genos Instituto Agroforestal Mediterr\u00e1neo Universidad Polit\u00e9cnica de Valencia Camino de Vera s\/n 46022 Valencia Spain"}]},{"given":"A. 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