{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T02:09:57Z","timestamp":1775873397736,"version":"3.50.1"},"reference-count":72,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,9,22]],"date-time":"2009-09-22T00:00:00Z","timestamp":1253577600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2009,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Endospores formed by <jats:italic>Bacillus subtilis<\/jats:italic> are encased in a tough protein shell known as the coat, which consists of at least 70 different proteins. We investigated the process of spore coat morphogenesis using a library of 40 coat proteins fused to green fluorescent protein and demonstrate that two successive steps can be distinguished in coat assembly. The first step, initial localization of proteins to the spore surface, is dependent on the coat morphogenetic proteins SpoIVA and SpoVM. The second step, spore encasement, requires a third protein, SpoVID. We show that in <jats:italic>spoVID<\/jats:italic> mutant cells, most coat proteins assembled into a cap at one side of the developing spore but failed to migrate around and encase it. We also found that SpoIVA directly interacts with SpoVID. A domain analysis revealed that the N\u2010terminus of SpoVID is required for encasement and is a structural homologue of a virion protein, whereas the C\u2010terminus is necessary for the interaction with SpoIVA. Thus, SpoVM, SpoIVA and SpoVID are recruited to the spore surface in a concerted manner and form a tripartite machine that drives coat formation and spore encasement.<\/jats:p>","DOI":"10.1111\/j.1365-2958.2009.06886.x","type":"journal-article","created":{"date-parts":[[2009,9,23]],"date-time":"2009-09-23T02:32:45Z","timestamp":1253673165000},"page":"634-649","source":"Crossref","is-referenced-by-count":74,"title":["The coat morphogenetic protein SpoVID is necessary for spore encasement in <i>Bacillus subtilis<\/i>"],"prefix":"10.1111","volume":"74","author":[{"given":"Katherine H.","family":"Wang","sequence":"first","affiliation":[]},{"given":"Anabela L.","family":"Isidro","sequence":"additional","affiliation":[]},{"given":"Lia","family":"Domingues","sequence":"additional","affiliation":[]},{"given":"Haig A.","family":"Eskandarian","sequence":"additional","affiliation":[]},{"given":"Peter 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