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Besides cytochrome<jats:italic>c<\/jats:italic>oxidase, plant mitochondria have an alternative oxidase (AOX) that accepts electrons directly from ubiquinol, dissipating energy as heat. AOX upregulation occurs under a variety of stresses and its induction by salicylic acid (SA) has been observed in different plant species. AOX was also suggested to be used as a functional marker for cell reprogramming under stress. In the present study, we have used etiolated soybean (<jats:italic>Glycine max<\/jats:italic>(L.) Merr. cv Cresir) seedlings to study the effects of SA treatment on the lipid composition and the respiratory properties of hypocotyl mitochondria. AOX expression was studied in detail, as a reporter protein, to evaluate whether modifications in mitochondrial energy metabolism were occurring. In mitochondria extracted from SA\u2010treated seedlings, AOX capacity and protein contents increased. Both<jats:italic>AOX1<\/jats:italic>and<jats:italic>AOX2b<\/jats:italic>transcripts accumulated in response to SA, but with different kinetics. A reduction in external NADH oxidation capacity was observed, whereas succinate respiration remained unchanged. The phospholipid composition of mitochondria remained similar in control and SA\u2010treated plants, but a reduction in the relative amount of linolenic acid was observed in phosphatidylcholine, phosphatidylethanolamine and cardiolipin. The possible causes of the fatty acid modifications observed, and the implications for mitochondrial metabolism are discussed.<\/jats:p>","DOI":"10.1111\/j.1399-3054.2009.01250.x","type":"journal-article","created":{"date-parts":[[2009,5,5]],"date-time":"2009-05-05T13:58:05Z","timestamp":1241531885000},"page":"485-497","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Study of the effects of salicylic acid on soybean mitochondrial lipids and respiratory properties using the alternative oxidase as a stress\u2010reporter protein"],"prefix":"10.1111","volume":"137","author":[{"given":"Ana Rita","family":"Matos","sequence":"first","affiliation":[]},{"given":"Ana Teresa","family":"Mendes","sequence":"additional","affiliation":[]},{"given":"Paula","family":"Scotti\u2010Campos","sequence":"additional","affiliation":[]},{"given":"Jo\u00e3o 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