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Antioxidant capacity was assessed using FRAP and DPPH assays, while bioactivity was further evaluated through elastase and hyaluronidase inhibition assays.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Total phenolic content ranged from 35.2 to 89.6\u2009mg GAE\/g DW, with Sous\u00e3o exhibiting the highest values. Ortho\u2010diphenols and flavonoids showed marked cultivar\u2010dependent variability, with Verdelho and Cercial presenting values above 20\u2009mg CAE\/g DW and 15\u2009mg QE\/g DW, respectively. Antioxidant activity reached up to 920\u2009\u03bcmol TE\/g DW (FRAP) and 85% DPPH radical scavenging. Enzyme inhibition assays revealed strong cultivar\u2010dependent bioactivity, with Sous\u00e3o, Verdelho, and Gouveio extracts achieving over 60% inhibition of elastase and hyaluronidase.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The results highlight the significant influence of grape cultivar on the bioactive potential of grape stems and support their valorization as promising raw materials for nutraceutical and cosmeceutical applications.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jocd.70762","type":"journal-article","created":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T06:41:47Z","timestamp":1773988907000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Inhibitory Effects of Grape Stem Extracts From Douro Varieties on Skin\u2010Aging Enzymes: A Sustainable Source of Cosmeceutical Agents"],"prefix":"10.1111","volume":"25","author":[{"given":"Maria","family":"Garcia\u2010Marti","sequence":"first","affiliation":[{"name":"Departamento de Qu\u00edmica Anal\u00edtica e Alimentaria, Facultade de Ciencias Universidade de Vigo  Ourense Spain"}]},{"given":"Oumaima","family":"Boutaub","sequence":"additional","affiliation":[{"name":"Centre for Research and Technology of Agro\u2010Environmental and Biological Sciences (CITAB) University of Tr\u00e1s\u2010os\u2010Montes and Alto Douro (UTAD)  Vila Real Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3445-5479","authenticated-orcid":false,"given":"Amanda Priscila Silva","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Academic Unit of Food Engineering Federal University of Campina Grande  Campina Grande PB Brazil"}]},{"given":"Jesus","family":"Simal\u2010Gandara","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica Anal\u00edtica e Alimentaria, Facultade de 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