{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T13:01:42Z","timestamp":1775912502235,"version":"3.50.1"},"reference-count":57,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T00:00:00Z","timestamp":1726012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["5R01AI116895"],"award-info":[{"award-number":["5R01AI116895"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["5R01AI172043"],"award-info":[{"award-number":["5R01AI172043"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>YabG is a sporulation\u2010specific protease that is conserved among sporulating bacteria. <jats:italic>Clostridioides difficile<\/jats:italic> YabG processes the cortex destined proteins preproSleC into proSleC and CspBA to CspB and CspA. YabG also affects synthesis of spore coat\/exosporium proteins CotA and CdeM. In prior work that identified CspA as the co\u2010germinant receptor, mutations in <jats:italic>yabG<\/jats:italic> were found which altered the co\u2010germinants required to initiate spore germination. To understand how these mutations in the <jats:italic>yabG<\/jats:italic> locus contribute to <jats:italic>C<\/jats:italic>. <jats:italic>difficile<\/jats:italic> spore germination, we introduced these mutations into an isogenic background. Spores derived from <jats:italic>C<\/jats:italic>. <jats:italic>difficile yabG<\/jats:italic><jats:sub>C207A<\/jats:sub> (a catalytically inactive allele), <jats:italic>C<\/jats:italic>. <jats:italic>difficile yabG<\/jats:italic><jats:sub>A46D<\/jats:sub>, <jats:italic>C<\/jats:italic>. <jats:italic>difficile yabG<\/jats:italic><jats:sub>G37E<\/jats:sub>, and <jats:italic>C<\/jats:italic>. <jats:italic>difficile yabG<\/jats:italic><jats:sub>P153L<\/jats:sub> strains germinated in response to taurocholic acid alone. Recombinantly expressed and purified preproSleC incubated with <jats:italic>E<\/jats:italic>. <jats:italic>coli<\/jats:italic> lysate expressing wild type YabG resulted in the removal of the presequence from preproSleC. Interestingly, only YabG<jats:sub>A46D<\/jats:sub> showed any activity toward purified preproSleC. Mutation of the YabG processing site in preproSleC (R119A) led to YabG shifting its processing to R115 or R112. Finally, changes in <jats:italic>yabG<\/jats:italic> expression under the mutant promoters were analyzed using a SNAP\u2010tag and revealed expression differences at early and late stages of sporulation. Overall, our results support and expand upon the hypothesis that YabG is important for germination and spore assembly and, upon mutation of the processing site, can shift where it cleaves substrates.<\/jats:p>","DOI":"10.1111\/mmi.15316","type":"journal-article","created":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T09:45:55Z","timestamp":1726047955000},"page":"534-548","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The Impact of <scp>YabG<\/scp> Mutations on <i>Clostridioides difficile<\/i> Spore Germination and Processing of Spore Substrates"],"prefix":"10.1111","volume":"122","author":[{"given":"Morgan\u00a0S.","family":"Osborne","sequence":"first","affiliation":[{"name":"Department of Biology Texas A&amp;M University  College Station Texas USA"}]},{"given":"Joshua\u00a0N.","family":"Brehm","sequence":"additional","affiliation":[{"name":"Department of Biology Texas A&amp;M University  College Station 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