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In plants, <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>tdIns(3,5)<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content><jats:sub>2<\/jats:sub> is synthesized by the <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>ab1 family, four orthologs of which exist in <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>rabidopsis: <jats:styled-content style=\"fixed-case\">FAB<\/jats:styled-content>1<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">FAB<\/jats:styled-content>1<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>, both from the <jats:styled-content style=\"fixed-case\">PIK<\/jats:styled-content>fyve\/<jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>ab1 family; <jats:styled-content style=\"fixed-case\">FAB<\/jats:styled-content>1<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">FAB<\/jats:styled-content>1<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>, both without a <jats:styled-content style=\"fixed-case\">PIK<\/jats:styled-content>fyve domain and of unclear role.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Using a reverse genetics and cell biology approach, we investigated the function of the <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>rabidopsis genes encoding <jats:styled-content style=\"fixed-case\">FAB<\/jats:styled-content>1<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">FAB<\/jats:styled-content>1<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>, both highly expressed in pollen.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>Pollen viability, germination and tube morphology were not significantly affected in homozygous mutant plants. <jats:italic>In\u00a0vivo<\/jats:italic>, mutant pollen fertilized ovules leading to normal seeds and siliques. The same result was obtained when mutant ovules were fertilized with wild\u2010type pollen. Double mutant pollen for the two genes was able to fertilize and develop plants no different from the wild\u2010type.<\/jats:p><\/jats:list-item>\n\n<jats:list-item><jats:p>At the cellular level, <jats:italic>fab1b<\/jats:italic> and <jats:italic>fab1d<\/jats:italic> pollen tubes were found to exhibit perturbations in membrane recycling, vacuolar acidification and decreased production of reactive oxygen species (<jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>). Subcellular imaging of <jats:styled-content style=\"fixed-case\">FAB<\/jats:styled-content>1<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content> revealed that the protein localized to the endomembrane compartment, whereas <jats:styled-content style=\"fixed-case\">FAB<\/jats:styled-content>1<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content> localized mostly to the cytosol and sperm cells. These results were discussed considering possible complementary roles of <jats:styled-content style=\"fixed-case\">FAB<\/jats:styled-content>1<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">FAB<\/jats:styled-content>1<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>.<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1111\/nph.12836","type":"journal-article","created":{"date-parts":[[2014,5,8]],"date-time":"2014-05-08T09:11:15Z","timestamp":1399540275000},"page":"784-793","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Characterization of <scp>FAB<\/scp>1 phosphatidylinositol kinases in <scp>A<\/scp>rabidopsis pollen tube growth and fertilization"],"prefix":"10.1111","volume":"203","author":[{"given":"Susana","family":"Serrazina","sequence":"first","affiliation":[{"name":"Faculdade de Ci\u00eancias de Lisboa BioFIG 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