{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T14:57:06Z","timestamp":1769525826317,"version":"3.49.0"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T00:00:00Z","timestamp":1760832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100016330","name":"Henan Provincial People's Hospital","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100016330","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Oral Microbiology"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Microbial infections and lipopolysaccharide (LPS)\u2010induced senescence in human dental pulp cells (hDPCs) play a significant role in gingivitis etiology. However, the role of Apelin\u201012 in oral diseases, particularly its modulation of cellular senescence, remains poorly understood. This study investigated the protective effects of Apelin\u201012 against LPS\u2010induced cellular senescence in hDPCs and its underlying mechanisms using cell isolation, culture, treatment, and transduction techniques, combined with reverse transcription\u2010polymerase chain reaction (RT\u2010PCR), Western blotting, telomerase activity assays, senescence\u2010associated \u03b2\u2010galactosidase (SA\u2010\u03b2\u2010Gal) staining, and gene silencing. We first confirmed apelin receptor (APJ) expression in hDPCs and found that LPS significantly downregulated APJ at both mRNA and protein levels. Apelin\u201012 treatment restored telomerase activity and upregulated human telomerase reverse transcriptase (hTERT), while reducing senescence markers, including \u03b3H2AX and SA\u2010\u03b2\u2010Gal. Additionally, Apelin\u201012 suppressed the expression of senescence regulators p21 and acetylated p53 (ac\u2010p53). Mechanistically, Apelin\u201012 restored SIRT6 (but not SIRT1) expression, and silencing SIRT6 abolished its anti\u2010senescence effects, as evidenced by elevated p21, ac\u2010p53, and SA\u2010\u03b2\u2010Gal, along with reduced hTERT and telomerase activity. These findings demonstrate that Apelin\u201012 attenuates LPS\u2010induced cellular senescence in hDPCs via SIRT6\u2010mediated pathways, suggesting its therapeutic potential for gingivitis management.<\/jats:p>","DOI":"10.1111\/omi.70012","type":"journal-article","created":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T14:42:13Z","timestamp":1760884933000},"page":"48-56","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Apelin\u201012 Attenuates LPS\u2010Induced Cellular Senescence in Human Dental Pulp Cells via SIRT6\u2010Mediated Pathways: Implications for Gingivitis Management"],"prefix":"10.1111","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-2604-064X","authenticated-orcid":false,"given":"Lin","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Stomatology Henan Provincial People's Hospital  Zhengzhou Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry Pingdingshan Stomatological Hospital  Pingdingshan Henan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Bai","sequence":"additional","affiliation":[{"name":"Department of Stomatology The First Affiliated Hospital of Henan University of Science and Technology  Luoyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345231154567"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2019.115220"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/ecosalplus.esp-0001-2018"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.203396"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.12.028"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12366"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reth.2019.12.002"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/biology11101419"},{"key":"e_1_2_10_10_1","first-page":"417","article-title":"Apelin\u2014A New Kid on the Block in Periodontology","volume":"22","author":"Cores Ziskoven 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