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The effect on the extracellular matrix of <jats:italic>P. aeruginosa<\/jats:italic> was also assessed. Irradiation with white light up to an energy dose of 64.8\u00a0J\u00a0cm<jats:sup>\u22122<\/jats:sup> in the presence of 20\u00a0<jats:italic>\u03bc<\/jats:italic><jats:sc>m<\/jats:sc> of Tetra\u2010Py<jats:sup>+<\/jats:sup>\u2010Me caused significant inactivation in all single\u2010species biofilms (3\u20136 log reductions), although the susceptibility was attenuated in relation to planktonic cells. In mixed biofilms, the inactivation of <jats:italic>S. aureus<\/jats:italic> was as efficient as in single\u2010species biofilms but the susceptibility of <jats:italic>C. albicans<\/jats:italic> decreased. In <jats:italic>P. aeruginosa<\/jats:italic> biofilms, a reduction of 81% in the polysaccharide content of the matrix was observed after treatment with a 20\u00a0<jats:italic>\u03bc<\/jats:italic><jats:sc>m <\/jats:sc><jats:styled-content style=\"fixed-case\">PS<\/jats:styled-content> concentration and a total light dose of 64.8\u00a0J\u00a0cm<jats:sup>\u22122<\/jats:sup>. The results show that the Tetra\u2010Py<jats:sup>+<\/jats:sup>\u2010Me causes significant inactivation of the microorganisms, either in biofilms or in the planktonic form, and demonstrate that polysaccharides of the biofilm matrix may be a primary target of photodynamic damage.<\/jats:p>","DOI":"10.1111\/php.12331","type":"journal-article","created":{"date-parts":[[2014,8,12]],"date-time":"2014-08-12T05:40:13Z","timestamp":1407822013000},"page":"1387-1396","source":"Crossref","is-referenced-by-count":116,"title":["Photodynamic Inactivation of Bacterial and Yeast Biofilms With a Cationic Porphyrin"],"prefix":"10.1111","volume":"90","author":[{"given":"Sandra","family":"Beir\u00e3o","sequence":"first","affiliation":[{"name":"Department of Biology and CESAM University of Aveiro Aveiro Portugal"}]},{"given":"Sara","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Department of Biology and CESAM University of Aveiro Aveiro Portugal"}]},{"given":"Joel","family":"Coelho","sequence":"additional","affiliation":[{"name":"Department of Chemistry and QOPNA University of Aveiro Aveiro Portugal"}]},{"given":"Maria A. 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