{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T21:46:57Z","timestamp":1771796817087,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"s1","license":[{"start":{"date-parts":[[2013,12,23]],"date-time":"2013-12-23T00:00:00Z","timestamp":1387756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biol J"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Plant roots are among most intensively studied biological systems in gravity research. Altered gravity induces asymmetric cell growth leading to root bending. Differential distribution of the phytohormone auxin underlies root responses to gravity, being coordinated by auxin efflux transporters from the <jats:styled-content style=\"fixed-case\">PIN<\/jats:styled-content> family. The objective of this study was to compare early transcriptomic changes in roots of <jats:italic>Arabidopsis thaliana<\/jats:italic> wild type, and <jats:italic>pin2<\/jats:italic> and <jats:italic>pin3<\/jats:italic> mutants under parabolic flight conditions and to correlate these changes to auxin distribution. Parabolic flights allow comparison of transient 1\u2212<jats:italic>g<\/jats:italic>, hypergravity and microgravity effects in living organisms in parallel. We found common and mutation\u2010related genes differentially expressed in response to transient microgravity phases. Gene ontology analysis of common genes revealed lipid metabolism, response to stress factors and light categories as primarily involved in response to transient microgravity phases, suggesting that fundamental reorganisation of metabolic pathways functions upstream of a further signal mediating hormonal network. Gene expression changes in roots lacking the columella\u2010located <jats:styled-content style=\"fixed-case\">PIN<\/jats:styled-content>3 were stronger than in those deprived of the epidermis and cortex cell\u2010specific <jats:styled-content style=\"fixed-case\">PIN<\/jats:styled-content>2. Moreover, repetitive exposure to microgravity\/hypergravity and gravity\/hypergravity flight phases induced an up\u2010regulation of auxin responsive genes in wild type and <jats:italic>pin2<\/jats:italic> roots, but not in <jats:italic>pin3<\/jats:italic> roots, suggesting a critical function of <jats:styled-content style=\"fixed-case\">PIN<\/jats:styled-content>3 in mediating auxin fluxes in response to transient microgravity phases. Our study provides important insights towards understanding signal transduction processes in transient microgravity conditions by combining for the first time the parabolic flight platform with the transcriptome analysis of different genetic mutants in the model plant, <jats:italic>Arabidopsis<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/plb.12130","type":"journal-article","created":{"date-parts":[[2013,12,24]],"date-time":"2013-12-24T03:37:52Z","timestamp":1387856272000},"page":"129-141","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Analysis of gene expression during parabolic flights reveals distinct early gravity responses in <i><scp>A<\/scp>rabidopsis<\/i> roots"],"prefix":"10.1111","volume":"16","author":[{"given":"D.","family":"Aubry\u2010Hivet","sequence":"first","affiliation":[{"name":"Institute of Biology II\/Botany Faculty of Biology Albert\u2010Ludwigs University of Freiburg Freiburg Germany"}]},{"given":"H.","family":"Nziengui","sequence":"additional","affiliation":[{"name":"Institute of Biology II\/Botany Faculty of Biology Albert\u2010Ludwigs University of Freiburg Freiburg Germany"}]},{"given":"K.","family":"Rapp","sequence":"additional","affiliation":[{"name":"Institute of Biology II\/Botany Faculty of Biology Albert\u2010Ludwigs University of Freiburg Freiburg Germany"}]},{"given":"O.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Institute of Biology II\/Botany Faculty of Biology Albert\u2010Ludwigs University of Freiburg Freiburg Germany"}]},{"given":"I. A.","family":"Paponov","sequence":"additional","affiliation":[{"name":"Institute of Biology II\/Botany Faculty of Biology Albert\u2010Ludwigs University of Freiburg Freiburg Germany"}]},{"given":"Y.","family":"Li","sequence":"additional","affiliation":[{"name":"Centre for Biological Systems Analysis (ZBSA) Albert\u2010Ludwigs University of Freiburg Freiburg Germany"}]},{"given":"J.","family":"Hauslage","sequence":"additional","affiliation":[{"name":"German Aerospace Center Institute of Aerospace Medicine Biomedical Science Support Center Cologne Germany"}]},{"given":"N.","family":"Vagt","sequence":"additional","affiliation":[{"name":"ESA\u2010ESTEC ISS Mission Science and Utilisation Planning Office Noordwijk The Netherlands"}]},{"given":"M.","family":"Braun","sequence":"additional","affiliation":[{"name":"Gravitational Biology Group Institute of Molecular Physiology and Biotechnology of Plants University of Bonn Bonn Germany"},{"name":"German Aerospace Center Space Administration Microgravity Research and Life Sciences Bonn Germany"}]},{"given":"F. 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