{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T14:06:43Z","timestamp":1765807603173},"reference-count":14,"publisher":"Wiley","issue":"S2","license":[{"start":{"date-parts":[[2018,9,21]],"date-time":"2018-09-21T00:00:00Z","timestamp":1537488000000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006301","name":"Center for International Business Education and Research, University of Illinois at Urbana-Champaign","doi-asserted-by":"publisher","award":["CB16\/11\/00494"],"award-info":[{"award-number":["CB16\/11\/00494"]}],"id":[{"id":"10.13039\/100006301","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010198","name":"Ministerio de Econom\u00eda, Industria y Competitividad, Gobierno de Espa\u00f1a","doi-asserted-by":"publisher","award":["AGL2015\u201073249\u2010JIN","AGL2015\u201066145","AGL2017\u201084681\u2010R"],"award-info":[{"award-number":["AGL2015\u201073249\u2010JIN","AGL2015\u201066145","AGL2017\u201084681\u2010R"]}],"id":[{"id":"10.13039\/501100010198","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Advanced age reduces the success of in vitro fertilization (<jats:styled-content style=\"fixed-case\">IVF<\/jats:styled-content>) being this effect partly mediated by an overproduction of reactive oxygen species (<jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>) that trigger apoptosis. It has been demonstrated that extracellular vesicles derived from endometrial mesenchymal stem cells (<jats:styled-content style=\"fixed-case\">EV<\/jats:styled-content>\u2010end<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s) exert an antioxidant effect and can be used as <jats:styled-content style=\"fixed-case\">IVF<\/jats:styled-content> coadjutants. In this work, end<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s were isolated from human menstrual blood (<jats:italic>n<\/jats:italic>\u00a0=\u00a04) and characterized according to multipotentiality and surface marker expression prior <jats:styled-content style=\"fixed-case\">EV<\/jats:styled-content>\u2010end<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s isolation. Oocytes were obtained from 21 B6D2 mice (24\u00a0weeks) and coincubated with sperm from young males (8\u201312\u00a0weeks). Presumptive zygotes were incubated in the presence of 0, 10, 20, 40 or 80\u00a0\u03bcg\/ml of <jats:styled-content style=\"fixed-case\">EV<\/jats:styled-content>\u2010end<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s in <jats:styled-content style=\"fixed-case\">KSOM<\/jats:styled-content> medium. Blastocyst yield was evaluated, and 25 blastocysts per group were used for <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>. Blastocyst rate was 29.4% in control; 45.2% for 10\u00a0\u03bcg\/ml, 62.9% for 20\u00a0\u03bcg\/ml, 55.5% for 40\u00a0\u03bcg\/ml and 53.8% in the 80\u00a0\u03bcg\/ml (<jats:italic>n<\/jats:italic>\u00a0=\u00a0124\u2013130 oocytes) being all the increases significantly different when compared against control (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). The 20\u201380\u00a0\u03bcg\/ml treatments decreased the expression of glutathione peroxidase (<jats:italic>Gpx1<\/jats:italic>), and the 10\u201340\u00a0\u03bcg\/ml treatments reduced the expression of superoxide dismutase (<jats:italic>Sod1<\/jats:italic>;<jats:italic> p<\/jats:italic>\u00a0&lt;\u00a00.05) compared to control; <jats:italic>Bax <\/jats:italic><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression did not vary. Our results suggest that the increased developmental competence of the embryos could be partly mediated by the <jats:styled-content style=\"fixed-case\">EV<\/jats:styled-content>\u2010end<jats:styled-content style=\"fixed-case\">MSC<\/jats:styled-content>s\u2019 <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content> scavenger activity.<\/jats:p>","DOI":"10.1111\/rda.13314","type":"journal-article","created":{"date-parts":[[2018,9,21]],"date-time":"2018-09-21T22:33:05Z","timestamp":1537569185000},"page":"46-49","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Extracellular vesicles derived from endometrial human mesenchymal stem cells improve IVF outcome in an aged murine model"],"prefix":"10.1111","volume":"53","author":[{"given":"Federica","family":"Marinaro","sequence":"first","affiliation":[{"name":"Jes\u00fas Us\u00f3n Minimally Invasive Surgery Centre (CCMIJU)  C\u00e1ceres Spain"}]},{"given":"Eva","family":"Pericuesta","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction INIA  Madrid Spain"}]},{"given":"Francisco Miguel","family":"S\u00e1nchez\u2010Margallo","sequence":"additional","affiliation":[{"name":"Jes\u00fas Us\u00f3n Minimally Invasive Surgery Centre (CCMIJU)  C\u00e1ceres Spain"},{"name":"CIBER de Enfermedades Cardiovasculares  Madrid Spain"}]},{"given":"Javier G.","family":"Casado","sequence":"additional","affiliation":[{"name":"Jes\u00fas Us\u00f3n Minimally Invasive Surgery Centre (CCMIJU)  C\u00e1ceres Spain"},{"name":"CIBER de Enfermedades Cardiovasculares  Madrid Spain"}]},{"given":"Ver\u00f3nica","family":"\u00c1lvarez","sequence":"additional","affiliation":[{"name":"Jes\u00fas Us\u00f3n Minimally Invasive Surgery Centre (CCMIJU)  C\u00e1ceres Spain"}]},{"given":"Elvira","family":"Matilla","sequence":"additional","affiliation":[{"name":"Jes\u00fas Us\u00f3n Minimally Invasive Surgery Centre (CCMIJU)  C\u00e1ceres Spain"}]},{"given":"Nuria","family":"Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Jes\u00fas Us\u00f3n Minimally Invasive Surgery Centre (CCMIJU)  C\u00e1ceres Spain"}]},{"given":"Rebeca","family":"Bl\u00e1zquez","sequence":"additional","affiliation":[{"name":"Jes\u00fas Us\u00f3n Minimally Invasive Surgery Centre (CCMIJU)  C\u00e1ceres Spain"},{"name":"CIBER de Enfermedades Cardiovasculares  Madrid Spain"}]},{"given":"Lauro","family":"Gonz\u00e1lez\u2010Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"SINTREP Group University of Extremadura  C\u00e1ceres Spain"}]},{"given":"Alfonso","family":"Guti\u00e9rrez\u2010Ad\u00e1n","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction INIA  Madrid Spain"}]},{"given":"Beatriz","family":"Mac\u00edas\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"Jes\u00fas Us\u00f3n Minimally Invasive Surgery Centre (CCMIJU)  C\u00e1ceres 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