{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T16:11:22Z","timestamp":1762445482648,"version":"3.41.2"},"reference-count":9,"publisher":"Wiley","issue":"S3","license":[{"start":{"date-parts":[[2024,10,13]],"date-time":"2024-10-13T00:00:00Z","timestamp":1728777600000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014181","name":"Junta de Extremadura","doi-asserted-by":"publisher","award":["IB20078, IB20005 and PI\u20100152\u201022"],"award-info":[{"award-number":["IB20078, IB20005 and PI\u20100152\u201022"]}],"id":[{"id":"10.13039\/501100014181","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100015720","name":"Universidad de Extremadura","doi-asserted-by":"publisher","award":["RC1"],"award-info":[{"award-number":["RC1"]}],"id":[{"id":"10.13039\/501100015720","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["IB20078 and IB20005"],"award-info":[{"award-number":["IB20078 and IB20005"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00f3n","doi-asserted-by":"publisher","award":["PID2020\u2010112723RB\u2010I00"],"award-info":[{"award-number":["PID2020\u2010112723RB\u2010I00"]}],"id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A protocol for conventional in\u00a0vitro fertilization (IVF) in horses using fresh semen has been described, using a prolonged incubation in FERT\u2010TALP medium (22\u2009h) at 38.2\u00b0C in the presence of penicillamine, hypotaurine and epinephrine (PHE). Our work aimed to develop a protocol that maintains quality parameters in frozen\u2013thawed equine spermatozoa incubated for 22\u2009h in the presence of PHE using different media (FERT\u2010TALP and INRA96) and incubation temperatures (30 and 38.2\u00b0C). Twelve frozen ejaculates from four stallions were thawed and then incubated in either FERT\u2010TALP or INRA96 with PHE at 30 or 38.2\u00b0C for 22\u2009h. Following incubation, total motility (TM), progressive motility (PM), viability and acrosome integrity were evaluated. The results showed that TM was significantly higher (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001) at 30\u00b0C in both media, while PM was higher for INRA96 at 30\u00b0C compared to 38\u00b0C (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). Moreover, INRA96 at 30\u00b0C exhibited higher sperm viability and acrosome integrity (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001) compared to the other experimental groups. These preliminary results suggest that incubating thawed equine spermatozoa at 30\u00b0C with PHE in INRA96 successfully maintains motility, viability and acrosome integrity in equine spermatozoa, indicating its potential use for conventional equine IVF.<\/jats:p>","DOI":"10.1111\/rda.14593","type":"journal-article","created":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T01:19:46Z","timestamp":1728868786000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Prolonged incubation of frozen\u2013thawed equine spermatozoa for in\u00a0vitro fertilization: A preliminary study using low temperature and <scp>INRA<\/scp>96 medium"],"prefix":"10.1111","volume":"59","author":[{"given":"Marcos","family":"Luis\u2010Calero","sequence":"first","affiliation":[{"name":"Departamento de Medicina Animal, Grupo de Investigaci\u00f3n Medicina Interna Veterinaria (MINVET), Instituto de Investigaci\u00f3n INBIO G+C, Facultad de Veterinaria Universidad de Extremadura  C\u00e1ceres Spain"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6733-4834","authenticated-orcid":false,"given":"Carmen C.","family":"Mu\u00f1oz\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Animal, Grupo de Investigaci\u00f3n Medicina Interna Veterinaria (MINVET), Instituto de Investigaci\u00f3n INBIO G+C, Facultad de Veterinaria Universidad de Extremadura  C\u00e1ceres Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5445-1630","authenticated-orcid":false,"given":"Pablo","family":"Fern\u00e1ndez\u2010Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Animal, Grupo de Investigaci\u00f3n Medicina Interna Veterinaria (MINVET), Instituto de Investigaci\u00f3n INBIO G+C, Facultad de Veterinaria Universidad de Extremadura  C\u00e1ceres Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5331-9793","authenticated-orcid":false,"given":"Beatriz","family":"Mac\u00edas\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Animal, Grupo de Investigaci\u00f3n Medicina Interna Veterinaria (MINVET), Instituto de Investigaci\u00f3n INBIO G+C, Facultad de Veterinaria Universidad de Extremadura  C\u00e1ceres Spain"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5568-548X","authenticated-orcid":false,"given":"Lauro","family":"Gonz\u00e1lez\u2010Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular y Gen\u00e9tica, Grupo de Investigaci\u00f3n Se\u00f1alizaci\u00f3n Intracelular y Tecnolog\u00eda de la Reproducci\u00f3n (SINTREP), Instituto de Investigaci\u00f3n INBIO G+C, Facultad de Veterinaria Universidad de Extremadura  C\u00e1ceres Spain"}]}],"member":"311","published-online":{"date-parts":[[2024,10,13]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_10_2_1","DOI":"10.1016\/j.jevs.2023.104499"},{"doi-asserted-by":"publisher","key":"e_1_2_10_3_1","DOI":"10.1530\/REP\u201011\u20100099"},{"doi-asserted-by":"publisher","key":"e_1_2_10_4_1","DOI":"10.1016\/j.jevs.2021.103801"},{"doi-asserted-by":"publisher","key":"e_1_2_10_5_1","DOI":"10.1093\/biolre\/ioac172"},{"doi-asserted-by":"publisher","key":"e_1_2_10_6_1","DOI":"10.1016\/j.jevs.2022.103875"},{"doi-asserted-by":"publisher","key":"e_1_2_10_7_1","DOI":"10.1155\/2016\/9380609"},{"doi-asserted-by":"publisher","key":"e_1_2_10_8_1","DOI":"10.1016\/j.theriogenology.2019.11.035"},{"doi-asserted-by":"publisher","key":"e_1_2_10_9_1","DOI":"10.1530\/REP\u201016\u20100420"},{"doi-asserted-by":"publisher","key":"e_1_2_10_10_1","DOI":"10.1016\/j.cryobiol.2017.10.005"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.14593","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T05:38:14Z","timestamp":1744868294000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.14593"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10]]},"references-count":9,"journal-issue":{"issue":"S3","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10.1111\/rda.14593"],"URL":"https:\/\/doi.org\/10.1111\/rda.14593","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"type":"print","value":"0936-6768"},{"type":"electronic","value":"1439-0531"}],"subject":[],"published":{"date-parts":[[2024,10]]},"assertion":[{"value":"2024-03-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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