{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T06:59:50Z","timestamp":1773817190941,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,3]],"date-time":"2026-01-03T00:00:00Z","timestamp":1767398400000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100008986","name":"University of Guelph","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008986","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Reprod Domestic Animals"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The main objective of this study was to assess the effects of donor age on the development of in\u00a0vitro\u2010derived sheep embryos. Ovaries were obtained after slaughter from cycling Polish Longwool ewes aged 1.5\u20133\u2009years (Group Y\u2014\u2018young\u2019;\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200914) or 8\u20139\u2009years (Group O\u2014\u2018old\u2019;\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200916). Cumulus\u2010oocyte complexes were collected and subjected to in\u00a0vitro maturation (IVM), followed by in\u00a0vitro fertilisation (IVF) with fresh capacitated ram semen. The resultant embryos were then cultured and monitored with a time\u2010lapse (TL) imaging system for up to 8\u2009days (Group Y,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200964 and Group O,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200948). The timing of key developmental stages relative to the moment when the oocytes and sperm were combined and including cleavage divisions as well as morula and blastocyst formation was recorded. Both the cleavage (68.75%) and blastocyst formation (26.6%) rates were significantly higher in younger ewes compared with their older counterparts (50.0% and 10.4%, respectively), with Group Y zygotes showing fewer (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) incidences of abnormal cleavage and morphology (fragmentation, direct cleavage or asymmetrical cleavage) compared with Group O (10.9% vs. 33.3%, respectively). The first cleavage division occurred earlier (25:42\u2009\u00b1\u20093:43 vs. 29:20\u2009\u00b1\u20096:59 [hours: minutes post\u2010insemination]; mean\u2009\u00b1\u2009SD;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) and the duration of the second cell cycle (time between the first and second mitotic division) was greater for Group Y compared with Group O (11:20\u2009\u00b1\u20099:51 vs. 4:14\u2009\u00b1\u20096:40;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). No significant differences were observed between the times of the following mitotic divisions or formation of morulae and blastocysts. This study documents the specific differences in embryo morphokinetics between donor ewes varying in age and highlights the usefulness of TL imaging for assessing the influence of maternal ageing on embryogenesis in sheep as a model for different mammalian species including humans.\n                  <\/jats:p>","DOI":"10.1111\/rda.70160","type":"journal-article","created":{"date-parts":[[2026,1,3]],"date-time":"2026-01-03T14:50:12Z","timestamp":1767451812000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Ewe Age on Embryo Viability and Morphokinetics: A Potential Ovine Model of Human Reproductive Ageing"],"prefix":"10.1111","volume":"61","author":[{"given":"Karolina","family":"Fryc","sequence":"first","affiliation":[{"name":"Department of Animal Biotechnology University of Agriculture in Krak\u00f3w  Cracow Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gee\u2010Zou","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Ontario Veterinary College University of Guelph  Guelph Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maciej","family":"Murawski","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology University of Agriculture in Krak\u00f3w  Cracow Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2731-9137","authenticated-orcid":false,"given":"Pawel M.","family":"Bartlewski","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Ontario Veterinary College University of Guelph  Guelph Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/af\/vfz017"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.14715\/cmb\/2017.63.8.18"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13048\u2010024\u201001427\u2010y"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioaf135"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2011.02.024"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1472\u20106483(10)61841\u2010x"},{"key":"e_1_2_9_8_1","volume-title":"Experimental Designs","author":"Cochran W. 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(Polish Pog\u00f3rze Sheep\u2014A Disappearing Part of the Landscape?)","volume":"4","author":"Kaczor U.","year":"2010","journal-title":"Wiadomo\u015bci Zootechniczne"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/00288233.2013.857698"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1642\u2010431x(12)60002\u20103"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2020.111377"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439\u20100531.2003.00437.x"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.14336\/AD.2021.1209"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/03009734.2020.1728444"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.104.038745"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439\u20100531.2008.01190.x"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2010.08.030"},{"key":"e_1_2_9_25_1","volume-title":"Age\u2010Related Fertility Decline","author":"Owen A.","year":"2025"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrd.1153"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10815\u2010011\u20109634\u20105"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13048\u2010023\u201001151\u2010z"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00686"}],"container-title":["Reproduction in Domestic Animals"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70160","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/rda.70160","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/rda.70160","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T05:59:09Z","timestamp":1773813549000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/rda.70160"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1111\/rda.70160"],"URL":"https:\/\/doi.org\/10.1111\/rda.70160","archive":["Portico"],"relation":{},"ISSN":["0936-6768","1439-0531"],"issn-type":[{"value":"0936-6768","type":"print"},{"value":"1439-0531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-08-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70160"}}