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The latter expresses five small rubber particle protein (<jats:styled-content style=\"fixed-case\">SRPP<\/jats:styled-content>) isoforms named Tb<jats:styled-content style=\"fixed-case\">SRPP<\/jats:styled-content>1\u20135, the most abundant proteins in rubber particles. These proteins maintain particle stability and are therefore necessary for rubber biosynthesis. <jats:italic>Tb<jats:styled-content style=\"fixed-case\">SRPP<\/jats:styled-content>1\u20135<\/jats:italic> were transiently expressed in <jats:italic>Nicotiana benthamiana<\/jats:italic> protoplasts and the proteins were found to be localized on lipid droplets and in the endoplasmic reticulum, with Tb<jats:styled-content style=\"fixed-case\">SRPP<\/jats:styled-content>1 and Tb<jats:styled-content style=\"fixed-case\">SRPP<\/jats:styled-content>3 also present in the cytosol. Bimolecular fluorescence complementation confirmed pairwise interactions between all proteins except Tb<jats:styled-content style=\"fixed-case\">SRPP<\/jats:styled-content>2. The corresponding genes showed diverse expression profiles in young <jats:italic>T<\/jats:italic>. <jats:italic>brevicorniculatum<\/jats:italic> plants exposed to abiotic stress, and all except <jats:italic>Tb<jats:styled-content style=\"fixed-case\">SRPP<\/jats:styled-content>4<\/jats:italic> and <jats:italic>Tb<jats:styled-content style=\"fixed-case\">SRPP<\/jats:styled-content>5<\/jats:italic> were upregulated. Young <jats:italic>Arabidopsis thaliana<\/jats:italic> plants that overexpressed <jats:italic>Tb<jats:styled-content style=\"fixed-case\">SRPP<\/jats:styled-content>2<\/jats:italic> and <jats:italic>Tb<jats:styled-content style=\"fixed-case\">SRPP<\/jats:styled-content>3<\/jats:italic> tolerated drought stress better than wild\u2010type plants. Furthermore, we used rubber particle extracts and standards to investigate the affinity of the Tb<jats:styled-content style=\"fixed-case\">SRPP<\/jats:styled-content>s for different phospholipids, revealing a preference for negatively charged head groups and 18:2\/16:0 fatty acid chains. This finding may explain the effect of Tb<jats:styled-content style=\"fixed-case\">SRPP<\/jats:styled-content>3\u20135 on the dispersity of artificial poly(<jats:italic>cis<\/jats:italic>\u20101,4\u2010isoprene) bodies and on the lipid droplet distribution we observed in <jats:italic>N<\/jats:italic>. <jats:italic>benthamiana<\/jats:italic> leaves. Our data provide insight into the assembly of Tb<jats:styled-content style=\"fixed-case\">SRPP<\/jats:styled-content>s on rubber particles, their role in rubber particle structure, and the link between rubber biosynthesis and lipid droplet\u2010associated stress responses, suggesting that <jats:styled-content style=\"fixed-case\">SRPP<\/jats:styled-content>s form the basis of evolutionarily conserved intracellular complexes in plants.<\/jats:p>","DOI":"10.1111\/tpj.13829","type":"journal-article","created":{"date-parts":[[2018,1,29]],"date-time":"2018-01-29T19:56:41Z","timestamp":1517255801000},"page":"1045-1061","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Small rubber particle proteins from <i>Taraxacum brevicorniculatum<\/i> promote stress tolerance and influence the size and distribution of lipid droplets and artificial poly(<i>cis<\/i>\u20101,4\u2010isoprene) bodies"],"prefix":"10.1111","volume":"93","author":[{"given":"Natalie","family":"Laibach","sequence":"first","affiliation":[{"name":"Fraunhofer Institute for Molecular Biology and Applied Ecology (IME) Schlossplatz 8 48143 M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sina","family":"Schmidl","sequence":"additional","affiliation":[{"name":"University of Muenster Institute of Plant Biology and Biotechnology Schlossplatz 8 48143 M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boje","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Molecular Biology and Applied Ecology (IME) Schlossplatz 8 48143 M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maike","family":"Bergmann","sequence":"additional","affiliation":[{"name":"University of Muenster Institute of Plant Biology and Biotechnology Schlossplatz 8 48143 M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Pr\u00fcfer","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Molecular Biology and Applied Ecology (IME) Schlossplatz 8 48143 M\u00fcnster Germany"},{"name":"University of Muenster Institute of Plant Biology and Biotechnology Schlossplatz 8 48143 M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Schulze Gronover","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Molecular Biology and Applied Ecology (IME) Schlossplatz 8 48143 M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00018060"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0048065"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2014.07.002"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2013.08.025"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/la301135z"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.12678"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/la104097n"},{"key":"e_1_2_6_10_1","unstructured":"2011 P. 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