{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T15:27:31Z","timestamp":1751383651495,"version":"3.40.5"},"reference-count":23,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2023,1,24]],"date-time":"2023-01-24T00:00:00Z","timestamp":1674518400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Ophthalmology"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine the morphology and volume of Meibomian glands (MG) of dogs with microCT before and after partial tarsal plate excision (PTPE), cryotherapy, and laser therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Procedure<\/jats:title><jats:p>MicroCT scans were made of 12 upper lids (ULs) and lower lids (LLs) of 12 dogs. After undergoing PTPE, 10 ULs and LLs were scanned again, and one UL and one LL was scanned after laser therapy and one UL and one LL after cryotherapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The length of the area containing MGs did not change pre\u2010 and post\u2010PTPE, and cryo\u2010 or laser therapy. The mean number of MGs in the ULs and LLs was 30.50 and 29.42, respectively, and did not change during the procedures. The average length of one individual MG was 2.60\u2009mm. The mean volume of MGs in the 10 ULs and LLs pre\u2010PTPE was 21.45 and 17.2\u2009mm<jats:sup>3<\/jats:sup>, respectively, and 12.84 and 11.25\u2009mm<jats:sup>3<\/jats:sup> in the UL and LL after PTPE, respectively. The mean volume of MGs decreased from 29.78\u2009mm<jats:sup>3<\/jats:sup> precryotherapy to 28.91\u2009mm<jats:sup>3<\/jats:sup> post\u2010treatment and in the lower eyelid from 22.87 to 22.4\u2009mm<jats:sup>3<\/jats:sup> after cryotherapy. The mean volume of MGs in the UL and LL before laser therapy was 8.95 and 6.78\u2009mm<jats:sup>3<\/jats:sup>, respectively, and after 9.25 and 6.38\u2009mm<jats:sup>3<\/jats:sup>, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>MicroCT is a valuable tool to determine the morphology and the volume of MGs and to demonstrate changes that occur after PTPE, laser\u2010, and cryotherapy. There is no need for additional preparation, such as staining, of the specimen prior to scanning.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vop.13057","type":"journal-article","created":{"date-parts":[[2023,1,24]],"date-time":"2023-01-24T11:29:04Z","timestamp":1674559744000},"page":"98-108","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Morphology and volume of Meibomian glands ex vivo pre and post partial tarsal plate excision, cryotherapy and laser therapy in the dog using <scp>microCT<\/scp>"],"prefix":"10.1111","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3503-998X","authenticated-orcid":false,"given":"Victoria","family":"Zwiauer\u2010Wolfbeisser","sequence":"first","affiliation":[{"name":"Department of Companion Animals and Horses University of Veterinary Medicine  Vienna 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