{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T18:49:29Z","timestamp":1761763769495},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:p>The transient rise of intracellular Ca<jats:sup>2+<\/jats:sup> in detrusor smooth muscle cells is due to the release of Ca<jats:sup>2+<\/jats:sup> from intracellular stores. However, it is not known how store refilling is maintained at a constant level to ensure constancy of the contractile response. The aim of these experiments was to characterise the role of L\u2010type Ca<jats:sup>2+<\/jats:sup> channels in refilling. Experiments used isolated guinea\u2010pig detrusor myocytes and store Ca<jats:sup>2+<\/jats:sup> content was estimated by measuring the magnitude of change to the intracellular [Ca<jats:sup>2+<\/jats:sup>] ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) after application of caffeine or carbachol using epifluorescence microscopy. Membrane potential was controlled when necessary by voltage clamp. After Ca<jats:sup>2+<\/jats:sup> stores were emptied they refilled with an exponential time course, with a time constant of 88 s. The value of the time constant was similar to that of the undershoot of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> following store Ca<jats:sup>2+<\/jats:sup> release. The degree of store filling was enhanced by maintained depolarisation, or by transient depolarising pulses, and attenuated by L\u2010type Ca<jats:sup>2+<\/jats:sup> channel antagonists. Inhibition of the sarcoplasmic reticular Ca<jats:sup>2+<\/jats:sup>\u2010ATPase prevented refilling. Reduction of the resting [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> was accompanied by membrane depolarisation; under voltage clamp reduction of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> decreased the number and magnitude of spontaneous transient outward currents. Ca<jats:sup>2+<\/jats:sup> release from intracellular stores, elicited by caffeine or carbachol, is independent of membrane potential under physiological conditions. However, store refilling occurs via Ca<jats:sup>2+<\/jats:sup> influx through L\u2010type Ca<jats:sup>2+<\/jats:sup> channels. Ca<jats:sup>2+<\/jats:sup> influx is regulated by a feedback mechanism whereby a fall of [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> reduces the activity of Ca<jats:sup>2+<\/jats:sup>\u2010activated K<jats:sup>+<\/jats:sup> channels, causing cell depolarisation and an enhancement of L\u2010type Ca<jats:sup>2+<\/jats:sup> channel conductance.<\/jats:p>","DOI":"10.1113\/jphysiol.2001.013191","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T12:45:57Z","timestamp":1027773957000},"page":"357-369","source":"Crossref","is-referenced-by-count":51,"title":["The role of the L\u2010type Ca<sup>2+<\/sup> channel in refilling functional intracellular Ca<sup>2+<\/sup> stores in guinea\u2010pig detrusor smooth muscle"],"prefix":"10.1113","volume":"538","author":[{"given":"C.","family":"Wu","sequence":"first","affiliation":[]},{"given":"G.","family":"Sui","sequence":"additional","affiliation":[]},{"given":"C. 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