{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T18:16:36Z","timestamp":1778177796304,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,2,7]],"date-time":"2005-02-07T00:00:00Z","timestamp":1107734400000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[2005,2]]},"abstract":"<jats:p>Glucagon\u2010like peptide\u20101 (GLP\u20101) is released from intestinal <jats:sc>L<\/jats:sc>\u2010cells in response to nutrient ingestion. It is currently under therapeutic evaluation because it enhances insulin secretion in type 2 diabetes. Previous studies using the GLP\u20101 secreting cell line GLUTag have shown that the cells are electrically active, and that the action potential frequency is regulated by nutrients. In this study we characterize voltage gated currents underlying this electrical activity and correlate the electrophysiological findings with gene expression determined by microarrays. Whole cell voltage clamp experiments designed to separate different ionic components revealed rapidly inactivating sodium currents sensitive to tetrodotoxin, calcium currents sensitive to nifedipine and \u03c9\u2010conotoxin GVIA, and sustained as well as rapidly inactivating potassium currents, which were sensitive to TEA and 4\u2010AP, respectively. In perforated patch experiments we also observed hyperpolarization\u2010activated currents which were inhibited by ZD7288. The amplitude of the sodium current was \u223c10 times that of the other depolarizing currents and tetrodotoxin abolished action potential firing. In secretion experiments, however, nifedipine, but not tetrodotoxin, \u03c9\u2010conotoxin GVIA or ZD7288, inhibited glucose\u2010induced GLP\u20101 release. Consistent with this finding, the intracellular Ca<jats:sup>2+<\/jats:sup> response to glucose was impaired by nifedipine but not by tetrodotoxin. Thus, in GLUTag cells, GLP\u20101 release is not dependent on the firing of Na<jats:sup>+<\/jats:sup>\u2010carrying action potentials but requires membrane depolarization and Ca<jats:sup>2+<\/jats:sup> entry through L\u2010type Ca<jats:sup>2+<\/jats:sup> channels. Understanding the characteristics of the currents and the molecular identification of the underlying channels in GLP\u20101 secreting cells might facilitate the development of agents to enhance GLP\u20101 secretion <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1113\/jphysiol.2004.076414","type":"journal-article","created":{"date-parts":[[2004,12,21]],"date-time":"2004-12-21T01:12:48Z","timestamp":1103591568000},"page":"161-175","source":"Crossref","is-referenced-by-count":53,"title":["Characterization and functional role of voltage gated cation conductances in the glucagon\u2010like peptide\u20101 secreting GLUTag cell line"],"prefix":"10.1113","volume":"563","author":[{"given":"F.","family":"Reimann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Maziarz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Flock","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. M.","family":"Habib","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. J.","family":"Drucker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. M.","family":"Gribble","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,7]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2003-0143"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.51.2007.S74"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature00887"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb06974.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.139.10.6228"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.16.1.521"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.139.4.2085"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.142.2.521"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1210\/me.8.12.1646"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.1992.tb01464.x"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.2001.281.3.G752"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.63.1.871"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.059675"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.52.5.1147"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.110.3.217"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83641-4"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.55.4.9"},{"key":"e_1_2_5_19_1","volume-title":"Ion Channels of Excitable Membranes","author":"Hille B","year":"2001"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1210\/er.20.6.876"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01296800"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.51.9.2757"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-004-1498-0"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.65.092101.142734"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.136.12.5593"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.140.4.1687"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001250050706"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1986.sp016096"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2000.00165.x"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00536.2003"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)07952-7"}],"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.2004.076414","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.2004.076414","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T12:52:13Z","timestamp":1697287933000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.2004.076414"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,2]]},"references-count":30,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2005,2]]}},"alternative-id":["10.1113\/jphysiol.2004.076414"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.2004.076414","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,2]]}}}