{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T02:34:11Z","timestamp":1777084451662,"version":"3.51.4"},"reference-count":56,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,3,31]],"date-time":"2015-03-31T00:00:00Z","timestamp":1427760000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004611","name":"Secret\u00e1rio de Ci\u00eancia, Tecnologia e Ensino Superior, Governo do Estado de Parana","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004611","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)"},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (Capes)"},{"name":"Fundacao Araucaria"},{"name":"Minist\u00e9rio da Ci\u00eancia, Tecnologia e Inova\u00e7\u00e3o (MCTI)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:sec><jats:title>New Findings<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item>\n<jats:p>What is the central question of this study?<\/jats:p>\n<jats:p>This study investigated the role of the endogenous anti\u2010inflammatory cytokine interleukin\u201010 in intense acute swimming\u2010induced muscle mechanical hyperalgesia in mice.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p>What is the main finding and its importance?<\/jats:p>\n<jats:p>Endogenous interleukin\u201010 has a key role in limiting exercise\u2010induced muscle pain in a model presenting similarities to delayed\u2010onset muscle soreness in mice. Interleukin\u201010 reduced muscle pain by diminishing leucocyte recruitment, hyperalgesic cytokine production, oxidative stress and myocyte damage.<\/jats:p>\n<\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>Interleukin\u201010 (IL\u201010) is an antihyperalgesic cytokine. In this study, IL\u201010\u2010deficient (IL\u201010<jats:sup>\u2212\/\u2212<\/jats:sup>) mice were used to investigate the role of endogenous IL\u201010 in intense acute swimming\u2010induced muscle mechanical hyperalgesia, which presents similarities with delayed\u2010onset muscle soreness. An intense acute swimming session of 1 or 2\u00a0h induced significant muscle mechanical hyperalgesia in a time\u2010dependent manner in wild\u2010type mice compared with the sham group 24\u00a0h after the session, which was further increased in IL\u201010<jats:sup>\u2212\/\u2212<\/jats:sup> mice (<jats:italic>P<\/jats:italic>\u00a0\u02c2\u00a00.05). Intraperitoneal treatment of wild\u2010type mice with IL\u201010 (1\u201310\u00a0ng) reduced muscle mechanical hyperalgesia in a dose\u2010dependent manner and reversed the enhanced muscle hyperalgesia in IL\u201010<jats:sup>\u2212\/\u2212<\/jats:sup> mice (<jats:italic>P<\/jats:italic>\u00a0\u02c2\u00a00.05). The 2\u00a0h swimming session induced increases in tumour necrosis factor\u2010\u03b1, interleukin\u20101\u03b2 and IL\u201010 production in the soleus muscle. However, tumour necrosis factor\u2010\u03b1 and interleukin\u20101\u03b2 production in the soleus muscle were even higher in IL\u201010<jats:sup>\u2212\/\u2212<\/jats:sup> mice between 2 and 6\u00a0h after the stimulus (<jats:italic>P<\/jats:italic>\u00a0\u02c2\u00a00.05). There was no statistical difference in the levels of the antihyperalgesic cytokines interleukin\u20104, interleukin\u20105, interleukin\u201013 and transforming growth factor\u2010\u03b2 between wild\u2010type and IL\u201010<jats:sup>\u2212\/\u2212<\/jats:sup> mice (<jats:italic>P<\/jats:italic>\u00a0\u02c3\u00a00.05). Interleukin\u201010 deficiency also resulted in increased myeloperoxidase activity, greater depletion of reduced glutathione levels, increased superoxide anion production and the maintenance of high plasma concentrations of creatine kinase (until 24\u00a0h after the swimming session) in soleus muscle (<jats:italic>P<\/jats:italic>\u00a0\u02c2\u00a00.05). These results demonstrate that endogenous IL\u201010 controls intense acute swimming\u2010induced muscle mechanical hyperalgesia by limiting oxidative stress and cytokine production.<\/jats:p><\/jats:sec>","DOI":"10.1113\/ep085026","type":"journal-article","created":{"date-parts":[[2015,2,25]],"date-time":"2015-02-25T08:10:49Z","timestamp":1424851849000},"page":"531-544","source":"Crossref","is-referenced-by-count":27,"title":["Interleukin\u201010 limits intense acute swimming\u2010induced muscle mechanical hyperalgesia in mice"],"prefix":"10.1113","volume":"100","author":[{"given":"Sergio M.","family":"Borghi","sequence":"first","affiliation":[{"name":"Departamento de Ci\u00eancias Patol\u00f3gicas Centro de Ci\u00eancias Biol\u00f3gicas Universidade Estadual de Londrina  Londrina Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felipe A.","family":"Pinho\u2010Ribeiro","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Patol\u00f3gicas Centro de Ci\u00eancias Biol\u00f3gicas Universidade Estadual de Londrina  Londrina Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana C.","family":"Zarpelon","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Patol\u00f3gicas Centro de Ci\u00eancias Biol\u00f3gicas Universidade Estadual de Londrina  Londrina Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thiago M.","family":"Cunha","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo  Ribeir\u00e3o Preto, S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose C.","family":"Alves\u2010Filho","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo  Ribeir\u00e3o Preto, S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio H.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo  Ribeir\u00e3o Preto, S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando Q.","family":"Cunha","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Ribeir\u00e3o Preto Medical School University of S\u00e3o Paulo  Ribeir\u00e3o Preto, S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rubia","family":"Casagrande","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Farmac\u00eauticas Centro de Ci\u00eancias da Sa\u00fade Universidade Estadual de Londrina  Londrina Paran\u00e1 Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"Waldiceu A.","family":"Verri","sequence":"additional","affiliation":[{"name":"Departamento de Ci\u00eancias Patol\u00f3gicas Centro de Ci\u00eancias Biol\u00f3gicas Universidade Estadual de Londrina  Londrina Paran\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,3,31]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.11.050"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3795-08.2008"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/np400222v"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.physbeh.2014.01.023"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jphp.12226"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1519\/JSC.0b013e3181d1d318"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2011007500050"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702266"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0100-879X2004000300018"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-014-8790-x"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00858.2006"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1089\/107999099313695"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/aet128"},{"key":"e_1_2_5_15_1","doi-asserted-by":"crossref","first-page":"3815","DOI":"10.4049\/jimmunol.147.11.3815","article-title":"IL\u201010 inhibits cytokine production by activated macrophages","volume":"147","author":"Fiorentino DF","year":"1990","journal-title":"J Immunol"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11916-003-0060-y"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0079445"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-9-278"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1321427111"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00421-013-2717-5"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3858-05.2006"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-009-0119-6"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(98)00027-7"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.111.233262"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neures.2012.02.005"},{"key":"e_1_2_5_26_1","first-page":"151","article-title":"Suppression of superoxide anion production by interleukin\u201010 is accompanied by a downregulation of the genes for subunit proteins of NADPH oxidase","volume":"24","author":"Kuga S","year":"1996","journal-title":"Exp Hematol"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.csm.2011.09.009"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpain.2006.06.010"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.2165\/00007256-199520010-00003"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-511X-7-24"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/1744-8069-1-9"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1532-2149.2013.00411.x"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2013.02.018"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2005.03.008"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2006.02.001"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1995.tb14987.x"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00031.2007"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.106.106351"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpain.2013.07.010"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11481-012-9428-2"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2011.01.026"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3959(03)00115-5"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apmr.2007.10.018"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2013.01.018"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1186\/1744-8069-5-75"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.2165\/00007256-200838110-00005"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.145.5.1435"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00470.2005"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00735.2009"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2007.07.031"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.22026"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.102.038703"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10974-012-9303-z"},{"key":"e_1_2_5_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2006.04.001"},{"key":"e_1_2_5_55_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2009.122655"},{"key":"e_1_2_5_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-012-0795-0"},{"key":"e_1_2_5_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2013.01.001"}],"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2FEP085026","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/EP085026","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T17:59:16Z","timestamp":1696183156000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/EP085026"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,3,31]]},"references-count":56,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,5]]}},"alternative-id":["10.1113\/EP085026"],"URL":"https:\/\/doi.org\/10.1113\/ep085026","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3,31]]}}}