{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T14:24:07Z","timestamp":1760711047608,"version":"3.37.3"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,12,26]],"date-time":"2016-12-26T00:00:00Z","timestamp":1482710400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:sec><jats:title>New Findings<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p><jats:bold>What is the central question of this study?<\/jats:bold><\/jats:p>\n<jats:p>What potential biochemical changes are associated with renal parenchyma 6\u201312\u00a0months after renal transplantation and delayed graft failure?<\/jats:p>\n<\/jats:list-item>\n<jats:list-item><jats:p><jats:bold>What is the main finding and its importance?<\/jats:bold><\/jats:p>\n<jats:p>Tissue fibrosis, mediated by tissue ischaemia\u2010induced induction of hypoxia\u2010inducible factor\u20101\u03b1 and fibronectin and consequent activation of lysyl oxidase, is a major underlying pathophysiological mechanism that contributes to delayed graft failure several months after renal transplantation.<\/jats:p>\n<\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>The present investigation was undertaken to evaluate the potential biochemical changes associated with renal parenchyma 6\u201312\u00a0months after renal transplantation and delayed graft failure. Serum concentrations of transforming growth factor\u2010\u03b2 in these subjects always remained elevated. In addition, examination of tissue from needle biopsies confirmed that there were consistent changes in the enzyme lysyl oxidase, which functions as an amine oxidase, modifies lysine residues on collagen and cross\u2010links in a process of modulation of the extracellular matrix. Parenchymal levels of hypoxia\u2010inducible factor\u20101\u03b1 and fibronectin were elevated, as detected by Western blotting. These findings indicate an ongoing ischaemic insult, which might result from increased tissue fibrosis or, in some cases, might be additive with pre\u2010existing pathophysiological factors that constrain proper renal haemodynamics. Thus, increased lysyl oxidase activity, which we assayed, is a potential unfavourable mechanism occurring in these kidneys that are undergoing failure and probably causes increased fibrosis within the organ and causes ischaemia, renovascular hypertension and a cascade leading to renal dysfunction and failure.<\/jats:p><\/jats:sec>","DOI":"10.1113\/ep085444","type":"journal-article","created":{"date-parts":[[2022,9,23]],"date-time":"2022-09-23T22:13:58Z","timestamp":1663971238000},"page":"282-287","source":"Crossref","is-referenced-by-count":4,"title":["Elevated ischaemia\u2010associated lysyl oxidase activity in delayed graft failure 6\u201312\u00a0months after renal transplantation"],"prefix":"10.1113","volume":"102","author":[{"given":"Zhi","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Nephrology The First Hospital of the Jilin University  Changchun 130021 Jilin 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130021 Jilin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology and Biophysics, Holden Comprehensive Cancer Center University of Iowa Carver College of Medicine  Iowa city IA 52242 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4521-6318","authenticated-orcid":false,"given":"Ying","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Nephrology The First Hospital of the Jilin University  Changchun 130021 Jilin 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